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Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice
Reactive α,β-unsaturated aldehydes such as acrolein (ACR) are major components of environmental pollutants and have been implicated in the neurodegenerative and cardiac diseases. In this study, the protective effect of silymarin (SN) against cardiotoxicity induced by ACR in mice was evaluated. Studi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3535759/ https://www.ncbi.nlm.nih.gov/pubmed/23320028 http://dx.doi.org/10.1155/2012/352091 |
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author | Taghiabadi, Elahe Imenshahidi, Mohsen Abnous, Khalil Mosafa, Fatemeh Sankian, Mojtaba Memar, Bahram Karimi, Gholamreza |
author_facet | Taghiabadi, Elahe Imenshahidi, Mohsen Abnous, Khalil Mosafa, Fatemeh Sankian, Mojtaba Memar, Bahram Karimi, Gholamreza |
author_sort | Taghiabadi, Elahe |
collection | PubMed |
description | Reactive α,β-unsaturated aldehydes such as acrolein (ACR) are major components of environmental pollutants and have been implicated in the neurodegenerative and cardiac diseases. In this study, the protective effect of silymarin (SN) against cardiotoxicity induced by ACR in mice was evaluated. Studies were performed on seven groups of six animals each, including vehicle-control (normal saline + 0.5% w/v methylcellulose), ACR (7.5 mg/kg/day, gavage) for 3 weeks, SN (25, 50 and 100 mg/kg/day, i.p.) plus ACR, vitamin E (Vit E, 100 IU/kg, i.p.) plus ACR, and SN (100 mg/kg, i.p.) groups. Mice received SN 7 days before ACR and daily thereafter throughout the study. Pretreatment with SN attenuated ACR-induced increased levels of malondialdehyde (MDA), serum cardiac troponin I (cTnI), and creatine kinase-MB (CK-MB), as well as histopathological changes in cardiac tissues. Moreover, SN improved glutathione (GSH) content, superoxide dismutase (SOD), and catalase (CAT) activities in heart of ACR-treated mice. Western blot analysis showed that SN pretreatment inhibited apoptosis provoked by ACR through decreasing Bax/Bcl-2 ratio, cytosolic cytochrome c content, and cleaved caspase-3 level in heart. In conclusion, SN may have protective effects against cardiotoxicity of ACR by reducing lipid peroxidation, renewing the activities of antioxidant enzymes, and preventing apoptosis. |
format | Online Article Text |
id | pubmed-3535759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-35357592013-01-14 Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice Taghiabadi, Elahe Imenshahidi, Mohsen Abnous, Khalil Mosafa, Fatemeh Sankian, Mojtaba Memar, Bahram Karimi, Gholamreza Evid Based Complement Alternat Med Research Article Reactive α,β-unsaturated aldehydes such as acrolein (ACR) are major components of environmental pollutants and have been implicated in the neurodegenerative and cardiac diseases. In this study, the protective effect of silymarin (SN) against cardiotoxicity induced by ACR in mice was evaluated. Studies were performed on seven groups of six animals each, including vehicle-control (normal saline + 0.5% w/v methylcellulose), ACR (7.5 mg/kg/day, gavage) for 3 weeks, SN (25, 50 and 100 mg/kg/day, i.p.) plus ACR, vitamin E (Vit E, 100 IU/kg, i.p.) plus ACR, and SN (100 mg/kg, i.p.) groups. Mice received SN 7 days before ACR and daily thereafter throughout the study. Pretreatment with SN attenuated ACR-induced increased levels of malondialdehyde (MDA), serum cardiac troponin I (cTnI), and creatine kinase-MB (CK-MB), as well as histopathological changes in cardiac tissues. Moreover, SN improved glutathione (GSH) content, superoxide dismutase (SOD), and catalase (CAT) activities in heart of ACR-treated mice. Western blot analysis showed that SN pretreatment inhibited apoptosis provoked by ACR through decreasing Bax/Bcl-2 ratio, cytosolic cytochrome c content, and cleaved caspase-3 level in heart. In conclusion, SN may have protective effects against cardiotoxicity of ACR by reducing lipid peroxidation, renewing the activities of antioxidant enzymes, and preventing apoptosis. Hindawi Publishing Corporation 2012 2012-12-18 /pmc/articles/PMC3535759/ /pubmed/23320028 http://dx.doi.org/10.1155/2012/352091 Text en Copyright © 2012 Elahe Taghiabadi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Taghiabadi, Elahe Imenshahidi, Mohsen Abnous, Khalil Mosafa, Fatemeh Sankian, Mojtaba Memar, Bahram Karimi, Gholamreza Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice |
title | Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice |
title_full | Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice |
title_fullStr | Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice |
title_full_unstemmed | Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice |
title_short | Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice |
title_sort | protective effect of silymarin against acrolein-induced cardiotoxicity in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3535759/ https://www.ncbi.nlm.nih.gov/pubmed/23320028 http://dx.doi.org/10.1155/2012/352091 |
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