Use of anionic denaturing detergents to purify insoluble proteins after overexpression

BACKGROUND: Many proteins form insoluble protein aggregates, called “inclusion bodies”, when overexpressed in E. coli. This is the biggest obstacle in biotechnology. Ever since the reversible denaturation of proteins by chaotropic agents such as urea or guanidinium hydrochloride had been shown, thes...

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Detalles Bibliográficos
Autores principales: Schlager, Benjamin, Straessle, Anna, Hafen, Ernst
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536628/
https://www.ncbi.nlm.nih.gov/pubmed/23231964
http://dx.doi.org/10.1186/1472-6750-12-95

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536628/
https://www.ncbi.nlm.nih.gov/pubmed/23231964
http://dx.doi.org/10.1186/1472-6750-12-95

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