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Use of anionic denaturing detergents to purify insoluble proteins after overexpression
BACKGROUND: Many proteins form insoluble protein aggregates, called “inclusion bodies”, when overexpressed in E. coli. This is the biggest obstacle in biotechnology. Ever since the reversible denaturation of proteins by chaotropic agents such as urea or guanidinium hydrochloride had been shown, thes...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2012
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536628/ https://www.ncbi.nlm.nih.gov/pubmed/23231964 http://dx.doi.org/10.1186/1472-6750-12-95 |