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Adipose tissue biglycan as a potential anti-inflammatory target of sodium salicylate in mice fed a high fat diet
BACKGROUND: Inflammation in adipose tissue (AT) during obesity causes impaired AT function. Although multiple extracellular matrix (ECM) proteins are expressed in AT their potential role in adipose tissue inflammation is unclear. Biglycan, a pro-inflammatory ECM gene, is highly enriched in adipose t...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536632/ https://www.ncbi.nlm.nih.gov/pubmed/22533381 http://dx.doi.org/10.1186/1476-9255-9-15 |
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author | Adapala, Venkata J Ward, Meliza Ajuwon, Kolapo M |
author_facet | Adapala, Venkata J Ward, Meliza Ajuwon, Kolapo M |
author_sort | Adapala, Venkata J |
collection | PubMed |
description | BACKGROUND: Inflammation in adipose tissue (AT) during obesity causes impaired AT function. Although multiple extracellular matrix (ECM) proteins are expressed in AT their potential role in adipose tissue inflammation is unclear. Biglycan, a pro-inflammatory ECM gene, is highly enriched in adipose tissue. However, whether it is correlated with adipose tissue inflammation is unknown. We provide evidence in support of a strong association between biglycan expression and inflammatory status of adipose tissue. METHODS: C57BL6 mice were fed either a control (10% fat calories) or a high fat diet (HFD) (60% fat calories) for 8 weeks. Adipose tissue was analyzed for the expression of biglycan, IL-6 and TNFα. Biglycan knockout or wild type were also fed a high fat diet for 8 weeks and the expression of inflammatory genes in the mesenteric adipose tissue was examined. To test anti-inflammatory treatment on biglycan expression, a group of mice were fed either the low fat or high fat diet for eight weeks supplemented with either saline or sodium salicylate @ 25mg/100ml in their drinking water. RESULTS: Mice on HFD had an increase in ECM genes (BGN and COL1A1), inflammatory genes (IL-6 and TNFα) in both the subcutaneous and epididymal depots. However, correlation analysis only shows a positive correlation between biglycan, IL-6 and TNFα expression. In addition, lower expression of IL-6 and CD68 was found in the mesenteric adipose tissue of biglycan knockout mice compared to the wild type. Sodium salicylate treatment reduced subcutaneous adipose tissue expression of BGN, COL1A1, and COL6A1 and a concurrent downregulation of TNFα and IL-6 and TLR4 expression. Salicylate also lowered the serum TGFβ1 levels. CONCLUSION: Biglycan expression correlates with adipose tissue inflammation, especially in the subcutaneous depot compared to the epididymal depot. This is supported by the greater effect of sodium salicylate in attenuating both inflammatory and ECM gene expression the subcutaneous adipose depot compared to the epididymal depot. These results show that inflammatory state may explain the induction of biglycan, and perhaps, other ECM genes in adipose tissue. |
format | Online Article Text |
id | pubmed-3536632 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35366322013-01-08 Adipose tissue biglycan as a potential anti-inflammatory target of sodium salicylate in mice fed a high fat diet Adapala, Venkata J Ward, Meliza Ajuwon, Kolapo M J Inflamm (Lond) Research BACKGROUND: Inflammation in adipose tissue (AT) during obesity causes impaired AT function. Although multiple extracellular matrix (ECM) proteins are expressed in AT their potential role in adipose tissue inflammation is unclear. Biglycan, a pro-inflammatory ECM gene, is highly enriched in adipose tissue. However, whether it is correlated with adipose tissue inflammation is unknown. We provide evidence in support of a strong association between biglycan expression and inflammatory status of adipose tissue. METHODS: C57BL6 mice were fed either a control (10% fat calories) or a high fat diet (HFD) (60% fat calories) for 8 weeks. Adipose tissue was analyzed for the expression of biglycan, IL-6 and TNFα. Biglycan knockout or wild type were also fed a high fat diet for 8 weeks and the expression of inflammatory genes in the mesenteric adipose tissue was examined. To test anti-inflammatory treatment on biglycan expression, a group of mice were fed either the low fat or high fat diet for eight weeks supplemented with either saline or sodium salicylate @ 25mg/100ml in their drinking water. RESULTS: Mice on HFD had an increase in ECM genes (BGN and COL1A1), inflammatory genes (IL-6 and TNFα) in both the subcutaneous and epididymal depots. However, correlation analysis only shows a positive correlation between biglycan, IL-6 and TNFα expression. In addition, lower expression of IL-6 and CD68 was found in the mesenteric adipose tissue of biglycan knockout mice compared to the wild type. Sodium salicylate treatment reduced subcutaneous adipose tissue expression of BGN, COL1A1, and COL6A1 and a concurrent downregulation of TNFα and IL-6 and TLR4 expression. Salicylate also lowered the serum TGFβ1 levels. CONCLUSION: Biglycan expression correlates with adipose tissue inflammation, especially in the subcutaneous depot compared to the epididymal depot. This is supported by the greater effect of sodium salicylate in attenuating both inflammatory and ECM gene expression the subcutaneous adipose depot compared to the epididymal depot. These results show that inflammatory state may explain the induction of biglycan, and perhaps, other ECM genes in adipose tissue. BioMed Central 2012-04-25 /pmc/articles/PMC3536632/ /pubmed/22533381 http://dx.doi.org/10.1186/1476-9255-9-15 Text en Copyright ©2012 Adapala et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Adapala, Venkata J Ward, Meliza Ajuwon, Kolapo M Adipose tissue biglycan as a potential anti-inflammatory target of sodium salicylate in mice fed a high fat diet |
title | Adipose tissue biglycan as a potential anti-inflammatory target of sodium salicylate in mice fed a high fat diet |
title_full | Adipose tissue biglycan as a potential anti-inflammatory target of sodium salicylate in mice fed a high fat diet |
title_fullStr | Adipose tissue biglycan as a potential anti-inflammatory target of sodium salicylate in mice fed a high fat diet |
title_full_unstemmed | Adipose tissue biglycan as a potential anti-inflammatory target of sodium salicylate in mice fed a high fat diet |
title_short | Adipose tissue biglycan as a potential anti-inflammatory target of sodium salicylate in mice fed a high fat diet |
title_sort | adipose tissue biglycan as a potential anti-inflammatory target of sodium salicylate in mice fed a high fat diet |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536632/ https://www.ncbi.nlm.nih.gov/pubmed/22533381 http://dx.doi.org/10.1186/1476-9255-9-15 |
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