Cargando…
Axonal trafficking of an antisense RNA transcribed from a pseudogene is regulated by classical conditioning
Natural antisense transcripts (NATs) are endogenous RNA molecules that are complementary to known RNA transcripts. The functional significance of NATs is poorly understood, but their prevalence in the CNS suggests a role in brain function. Here we investigated a long NAT (antiNOS-2 RNA) associated w...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3537157/ https://www.ncbi.nlm.nih.gov/pubmed/23293742 http://dx.doi.org/10.1038/srep01027 |
_version_ | 1782254842679394304 |
---|---|
author | Korneev, Sergei A. Kemenes, Ildiko Bettini, Natalia L. Kemenes, George Staras, Kevin Benjamin, Paul R. O'Shea, Michael |
author_facet | Korneev, Sergei A. Kemenes, Ildiko Bettini, Natalia L. Kemenes, George Staras, Kevin Benjamin, Paul R. O'Shea, Michael |
author_sort | Korneev, Sergei A. |
collection | PubMed |
description | Natural antisense transcripts (NATs) are endogenous RNA molecules that are complementary to known RNA transcripts. The functional significance of NATs is poorly understood, but their prevalence in the CNS suggests a role in brain function. Here we investigated a long NAT (antiNOS-2 RNA) associated with the regulation of nitric oxide (NO) production in the CNS of Lymnaea, an established model for molecular analysis of learning and memory. We show the antiNOS-2 RNA is axonally trafficked and demonstrate that this is regulated by classical conditioning. Critically, a single conditioning trial changes the amount of antiNOS-2 RNA transported along the axon. This occurs within the critical time window when neurotransmitter NO is required for memory formation. Our data suggest a role for the antiNOS-2 RNA in establishing memories through the regulation of NO signaling at the synapse. |
format | Online Article Text |
id | pubmed-3537157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-35371572013-01-04 Axonal trafficking of an antisense RNA transcribed from a pseudogene is regulated by classical conditioning Korneev, Sergei A. Kemenes, Ildiko Bettini, Natalia L. Kemenes, George Staras, Kevin Benjamin, Paul R. O'Shea, Michael Sci Rep Article Natural antisense transcripts (NATs) are endogenous RNA molecules that are complementary to known RNA transcripts. The functional significance of NATs is poorly understood, but their prevalence in the CNS suggests a role in brain function. Here we investigated a long NAT (antiNOS-2 RNA) associated with the regulation of nitric oxide (NO) production in the CNS of Lymnaea, an established model for molecular analysis of learning and memory. We show the antiNOS-2 RNA is axonally trafficked and demonstrate that this is regulated by classical conditioning. Critically, a single conditioning trial changes the amount of antiNOS-2 RNA transported along the axon. This occurs within the critical time window when neurotransmitter NO is required for memory formation. Our data suggest a role for the antiNOS-2 RNA in establishing memories through the regulation of NO signaling at the synapse. Nature Publishing Group 2013-01-04 /pmc/articles/PMC3537157/ /pubmed/23293742 http://dx.doi.org/10.1038/srep01027 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Korneev, Sergei A. Kemenes, Ildiko Bettini, Natalia L. Kemenes, George Staras, Kevin Benjamin, Paul R. O'Shea, Michael Axonal trafficking of an antisense RNA transcribed from a pseudogene is regulated by classical conditioning |
title | Axonal trafficking of an antisense RNA transcribed from a pseudogene is regulated by classical conditioning |
title_full | Axonal trafficking of an antisense RNA transcribed from a pseudogene is regulated by classical conditioning |
title_fullStr | Axonal trafficking of an antisense RNA transcribed from a pseudogene is regulated by classical conditioning |
title_full_unstemmed | Axonal trafficking of an antisense RNA transcribed from a pseudogene is regulated by classical conditioning |
title_short | Axonal trafficking of an antisense RNA transcribed from a pseudogene is regulated by classical conditioning |
title_sort | axonal trafficking of an antisense rna transcribed from a pseudogene is regulated by classical conditioning |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3537157/ https://www.ncbi.nlm.nih.gov/pubmed/23293742 http://dx.doi.org/10.1038/srep01027 |
work_keys_str_mv | AT korneevsergeia axonaltraffickingofanantisensernatranscribedfromapseudogeneisregulatedbyclassicalconditioning AT kemenesildiko axonaltraffickingofanantisensernatranscribedfromapseudogeneisregulatedbyclassicalconditioning AT bettininatalial axonaltraffickingofanantisensernatranscribedfromapseudogeneisregulatedbyclassicalconditioning AT kemenesgeorge axonaltraffickingofanantisensernatranscribedfromapseudogeneisregulatedbyclassicalconditioning AT staraskevin axonaltraffickingofanantisensernatranscribedfromapseudogeneisregulatedbyclassicalconditioning AT benjaminpaulr axonaltraffickingofanantisensernatranscribedfromapseudogeneisregulatedbyclassicalconditioning AT osheamichael axonaltraffickingofanantisensernatranscribedfromapseudogeneisregulatedbyclassicalconditioning |