In vitro substrate phosphorylation by Ca(2+)/calmodulin-dependent protein kinase kinase using guanosine-5(′)-triphosphate as a phosphate donor

BACKGROUND: Ca(2+)/calmodulin-dependent protein kinase kinase (CaMKK) phosphorylates and activates particular downstream protein kinases — including CaMKI, CaMKIV, and AMPK— to stimulate multiple Ca(2+)-signal transduction pathways. To identify previously unidentified CaMKK substrates, we used vario...

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Detalles Bibliográficos
Autores principales: Yurimoto, Saki, Fujimoto, Tomohito, Magari, Masaki, Kanayama, Naoki, Kobayashi, Ryoji, Tokumitsu, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3537517/
https://www.ncbi.nlm.nih.gov/pubmed/23216827
http://dx.doi.org/10.1186/1471-2091-13-27

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