Interfering growth of malignant melanoma with Ang2-siRNA

To investigate the intervention therapy effect on the growth of malignant melanoma, we have made an observation of expression levels of Ang2 in malignant melanoma cells, which was transduced by small interfering RNA (Ang2-siRNA) of Ang2 in vitro and in vivo. We successfully constructed Ang2-siRNA le...

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Detalles Bibliográficos
Autores principales: Wang, Biao, Liu, Zhaoliang, Zhang, Mingfeng, San, Xiuying, Zhang, Yanding, Zhang, Weiqiang, Wang, Meishui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538013/
https://www.ncbi.nlm.nih.gov/pubmed/23160899
http://dx.doi.org/10.1007/s11033-012-2189-4
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author Wang, Biao
Liu, Zhaoliang
Zhang, Mingfeng
San, Xiuying
Zhang, Yanding
Zhang, Weiqiang
Wang, Meishui
author_facet Wang, Biao
Liu, Zhaoliang
Zhang, Mingfeng
San, Xiuying
Zhang, Yanding
Zhang, Weiqiang
Wang, Meishui
author_sort Wang, Biao
collection PubMed
description To investigate the intervention therapy effect on the growth of malignant melanoma, we have made an observation of expression levels of Ang2 in malignant melanoma cells, which was transduced by small interfering RNA (Ang2-siRNA) of Ang2 in vitro and in vivo. We successfully constructed Ang2-siRNA lent virus, and constructed nude mice model by transplanting malignant melanoma. Ang2-siRNA lent virus inhibited Ang2 mRNA of malignant melanoma in vitro and in vivo, and inhibited malignant melanoma tumor growth at the same time. Ang2-siRNA lent virus can interfere expression levels of Ang2 in malignant melanoma cells, inhibit tumor growth, and silent Ang2 gene expression, which may pave a new way for clinical gene therapy of malignant melanoma.
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spelling pubmed-35380132013-01-09 Interfering growth of malignant melanoma with Ang2-siRNA Wang, Biao Liu, Zhaoliang Zhang, Mingfeng San, Xiuying Zhang, Yanding Zhang, Weiqiang Wang, Meishui Mol Biol Rep Article To investigate the intervention therapy effect on the growth of malignant melanoma, we have made an observation of expression levels of Ang2 in malignant melanoma cells, which was transduced by small interfering RNA (Ang2-siRNA) of Ang2 in vitro and in vivo. We successfully constructed Ang2-siRNA lent virus, and constructed nude mice model by transplanting malignant melanoma. Ang2-siRNA lent virus inhibited Ang2 mRNA of malignant melanoma in vitro and in vivo, and inhibited malignant melanoma tumor growth at the same time. Ang2-siRNA lent virus can interfere expression levels of Ang2 in malignant melanoma cells, inhibit tumor growth, and silent Ang2 gene expression, which may pave a new way for clinical gene therapy of malignant melanoma. Springer Netherlands 2012-11-16 2013 /pmc/articles/PMC3538013/ /pubmed/23160899 http://dx.doi.org/10.1007/s11033-012-2189-4 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Wang, Biao
Liu, Zhaoliang
Zhang, Mingfeng
San, Xiuying
Zhang, Yanding
Zhang, Weiqiang
Wang, Meishui
Interfering growth of malignant melanoma with Ang2-siRNA
title Interfering growth of malignant melanoma with Ang2-siRNA
title_full Interfering growth of malignant melanoma with Ang2-siRNA
title_fullStr Interfering growth of malignant melanoma with Ang2-siRNA
title_full_unstemmed Interfering growth of malignant melanoma with Ang2-siRNA
title_short Interfering growth of malignant melanoma with Ang2-siRNA
title_sort interfering growth of malignant melanoma with ang2-sirna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538013/
https://www.ncbi.nlm.nih.gov/pubmed/23160899
http://dx.doi.org/10.1007/s11033-012-2189-4
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