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Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces

Streptomyces are best known for producing antimicrobial secondary metabolites, but they are also recognized for their contributions to biomass utilization. Despite their importance to carbon cycling in terrestrial ecosystems, our understanding of the cellulolytic ability of Streptomyces is currently...

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Autores principales: Takasuka, Taichi E., Book, Adam J., Lewin, Gina R., Currie, Cameron R., Fox, Brian G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538285/
https://www.ncbi.nlm.nih.gov/pubmed/23301151
http://dx.doi.org/10.1038/srep01030
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author Takasuka, Taichi E.
Book, Adam J.
Lewin, Gina R.
Currie, Cameron R.
Fox, Brian G.
author_facet Takasuka, Taichi E.
Book, Adam J.
Lewin, Gina R.
Currie, Cameron R.
Fox, Brian G.
author_sort Takasuka, Taichi E.
collection PubMed
description Streptomyces are best known for producing antimicrobial secondary metabolites, but they are also recognized for their contributions to biomass utilization. Despite their importance to carbon cycling in terrestrial ecosystems, our understanding of the cellulolytic ability of Streptomyces is currently limited to a few soil-isolates. Here, we demonstrate the biomass-deconstructing capability of Streptomyces sp. SirexAA-E (ActE), an aerobic bacterium associated with the invasive pine-boring woodwasp Sirex noctilio. When grown on plant biomass, ActE secretes a suite of enzymes including endo- and exo-cellulases, CBM33 polysaccharide-monooxygenases, and hemicellulases. Genome-wide transcriptomic and proteomic analyses, and biochemical assays have revealed the key enzymes used to deconstruct crystalline cellulose, other pure polysaccharides, and biomass. The mixture of enzymes obtained from growth on biomass has biomass-degrading activity comparable to a cellulolytic enzyme cocktail from the fungus Trichoderma reesei, and thus provides a compelling example of high cellulolytic capacity in an aerobic bacterium.
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spelling pubmed-35382852013-01-08 Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces Takasuka, Taichi E. Book, Adam J. Lewin, Gina R. Currie, Cameron R. Fox, Brian G. Sci Rep Article Streptomyces are best known for producing antimicrobial secondary metabolites, but they are also recognized for their contributions to biomass utilization. Despite their importance to carbon cycling in terrestrial ecosystems, our understanding of the cellulolytic ability of Streptomyces is currently limited to a few soil-isolates. Here, we demonstrate the biomass-deconstructing capability of Streptomyces sp. SirexAA-E (ActE), an aerobic bacterium associated with the invasive pine-boring woodwasp Sirex noctilio. When grown on plant biomass, ActE secretes a suite of enzymes including endo- and exo-cellulases, CBM33 polysaccharide-monooxygenases, and hemicellulases. Genome-wide transcriptomic and proteomic analyses, and biochemical assays have revealed the key enzymes used to deconstruct crystalline cellulose, other pure polysaccharides, and biomass. The mixture of enzymes obtained from growth on biomass has biomass-degrading activity comparable to a cellulolytic enzyme cocktail from the fungus Trichoderma reesei, and thus provides a compelling example of high cellulolytic capacity in an aerobic bacterium. Nature Publishing Group 2013-01-07 /pmc/articles/PMC3538285/ /pubmed/23301151 http://dx.doi.org/10.1038/srep01030 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Takasuka, Taichi E.
Book, Adam J.
Lewin, Gina R.
Currie, Cameron R.
Fox, Brian G.
Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces
title Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces
title_full Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces
title_fullStr Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces
title_full_unstemmed Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces
title_short Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces
title_sort aerobic deconstruction of cellulosic biomass by an insect-associated streptomyces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538285/
https://www.ncbi.nlm.nih.gov/pubmed/23301151
http://dx.doi.org/10.1038/srep01030
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