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Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces
Streptomyces are best known for producing antimicrobial secondary metabolites, but they are also recognized for their contributions to biomass utilization. Despite their importance to carbon cycling in terrestrial ecosystems, our understanding of the cellulolytic ability of Streptomyces is currently...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538285/ https://www.ncbi.nlm.nih.gov/pubmed/23301151 http://dx.doi.org/10.1038/srep01030 |
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author | Takasuka, Taichi E. Book, Adam J. Lewin, Gina R. Currie, Cameron R. Fox, Brian G. |
author_facet | Takasuka, Taichi E. Book, Adam J. Lewin, Gina R. Currie, Cameron R. Fox, Brian G. |
author_sort | Takasuka, Taichi E. |
collection | PubMed |
description | Streptomyces are best known for producing antimicrobial secondary metabolites, but they are also recognized for their contributions to biomass utilization. Despite their importance to carbon cycling in terrestrial ecosystems, our understanding of the cellulolytic ability of Streptomyces is currently limited to a few soil-isolates. Here, we demonstrate the biomass-deconstructing capability of Streptomyces sp. SirexAA-E (ActE), an aerobic bacterium associated with the invasive pine-boring woodwasp Sirex noctilio. When grown on plant biomass, ActE secretes a suite of enzymes including endo- and exo-cellulases, CBM33 polysaccharide-monooxygenases, and hemicellulases. Genome-wide transcriptomic and proteomic analyses, and biochemical assays have revealed the key enzymes used to deconstruct crystalline cellulose, other pure polysaccharides, and biomass. The mixture of enzymes obtained from growth on biomass has biomass-degrading activity comparable to a cellulolytic enzyme cocktail from the fungus Trichoderma reesei, and thus provides a compelling example of high cellulolytic capacity in an aerobic bacterium. |
format | Online Article Text |
id | pubmed-3538285 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-35382852013-01-08 Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces Takasuka, Taichi E. Book, Adam J. Lewin, Gina R. Currie, Cameron R. Fox, Brian G. Sci Rep Article Streptomyces are best known for producing antimicrobial secondary metabolites, but they are also recognized for their contributions to biomass utilization. Despite their importance to carbon cycling in terrestrial ecosystems, our understanding of the cellulolytic ability of Streptomyces is currently limited to a few soil-isolates. Here, we demonstrate the biomass-deconstructing capability of Streptomyces sp. SirexAA-E (ActE), an aerobic bacterium associated with the invasive pine-boring woodwasp Sirex noctilio. When grown on plant biomass, ActE secretes a suite of enzymes including endo- and exo-cellulases, CBM33 polysaccharide-monooxygenases, and hemicellulases. Genome-wide transcriptomic and proteomic analyses, and biochemical assays have revealed the key enzymes used to deconstruct crystalline cellulose, other pure polysaccharides, and biomass. The mixture of enzymes obtained from growth on biomass has biomass-degrading activity comparable to a cellulolytic enzyme cocktail from the fungus Trichoderma reesei, and thus provides a compelling example of high cellulolytic capacity in an aerobic bacterium. Nature Publishing Group 2013-01-07 /pmc/articles/PMC3538285/ /pubmed/23301151 http://dx.doi.org/10.1038/srep01030 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Takasuka, Taichi E. Book, Adam J. Lewin, Gina R. Currie, Cameron R. Fox, Brian G. Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces |
title | Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces |
title_full | Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces |
title_fullStr | Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces |
title_full_unstemmed | Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces |
title_short | Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces |
title_sort | aerobic deconstruction of cellulosic biomass by an insect-associated streptomyces |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538285/ https://www.ncbi.nlm.nih.gov/pubmed/23301151 http://dx.doi.org/10.1038/srep01030 |
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