Cargando…
The DNA Copy Number of Human Endogenous Retrovirus-W (MSRV-Type) Is Increased in Multiple Sclerosis Patients and Is Influenced by Gender and Disease Severity
BACKGROUND: Multiple Sclerosis is an autoimmune disease more prevalent in women than in men. Multiple Sclerosis Associated Retrovirus element (MSRV) is a member of type-W endogenous retrovirus family (HERV-W), known to be associated to MS. Most HERVs are unable to replicate but MSRV expression assoc...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538585/ https://www.ncbi.nlm.nih.gov/pubmed/23308264 http://dx.doi.org/10.1371/journal.pone.0053623 |
_version_ | 1782254969235177472 |
---|---|
author | Garcia-Montojo, Marta Dominguez-Mozo, María Arias-Leal, Ana Garcia-Martinez, Ángel De las Heras, Virginia Casanova, Ignacio Faucard, Raphaël Gehin, Nadège Madeira, Alexandra Arroyo, Rafael Curtin, François Alvarez-Lafuente, Roberto Perron, Hervé |
author_facet | Garcia-Montojo, Marta Dominguez-Mozo, María Arias-Leal, Ana Garcia-Martinez, Ángel De las Heras, Virginia Casanova, Ignacio Faucard, Raphaël Gehin, Nadège Madeira, Alexandra Arroyo, Rafael Curtin, François Alvarez-Lafuente, Roberto Perron, Hervé |
author_sort | Garcia-Montojo, Marta |
collection | PubMed |
description | BACKGROUND: Multiple Sclerosis is an autoimmune disease more prevalent in women than in men. Multiple Sclerosis Associated Retrovirus element (MSRV) is a member of type-W endogenous retrovirus family (HERV-W), known to be associated to MS. Most HERVs are unable to replicate but MSRV expression associated with reverse-transcriptase activity in MS would explain reported DNA copy number increase in MS patients. A potential link between HERV-W copies on chromosome X and gender differential prevalence has been suggested. The present study addresses MSRV-type DNA load in relation with the gender differences and clinical status in MS and healthy controls. RESULTS: 178 MS patients (62.9% women) and 124 controls (56.5% women) were included. MSRV env load (copies/pg of DNA) was analyzed by real time qPCR with specific primers and probe for its env gene, in DNA from peripheral blood mononuclear cells (PBMCs). MSRV load was more elevated in MS patients than in controls (p = 4.15e-7). MS women presented higher MSRV load than control women (p = 0.009) and MS men also had higher load than control men (p = 2.77e-6). Besides, women had higher levels than men, both among patients (p = 0.007) and controls (p = 1.24e-6). Concordantly, EDSS and MSSS scores were higher among female patients with an elevated MSRV load (p = 0.03 and p = 0.04, respectively). CONCLUSIONS: MSRV increases its copy number in PBMC of MS patients and particularly in women with high clinical scores. This may explain causes underlying the higher prevalence of MS in women. The association with the clinical severity calls for further investigations on MSRV load in PBMCs as a biomarker for MS. |
format | Online Article Text |
id | pubmed-3538585 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35385852013-01-10 The DNA Copy Number of Human Endogenous Retrovirus-W (MSRV-Type) Is Increased in Multiple Sclerosis Patients and Is Influenced by Gender and Disease Severity Garcia-Montojo, Marta Dominguez-Mozo, María Arias-Leal, Ana Garcia-Martinez, Ángel De las Heras, Virginia Casanova, Ignacio Faucard, Raphaël Gehin, Nadège Madeira, Alexandra Arroyo, Rafael Curtin, François Alvarez-Lafuente, Roberto Perron, Hervé PLoS One Research Article BACKGROUND: Multiple Sclerosis is an autoimmune disease more prevalent in women than in men. Multiple Sclerosis Associated Retrovirus element (MSRV) is a member of type-W endogenous retrovirus family (HERV-W), known to be associated to MS. Most HERVs are unable to replicate but MSRV expression associated with reverse-transcriptase activity in MS would explain reported DNA copy number increase in MS patients. A potential link between HERV-W copies on chromosome X and gender differential prevalence has been suggested. The present study addresses MSRV-type DNA load in relation with the gender differences and clinical status in MS and healthy controls. RESULTS: 178 MS patients (62.9% women) and 124 controls (56.5% women) were included. MSRV env load (copies/pg of DNA) was analyzed by real time qPCR with specific primers and probe for its env gene, in DNA from peripheral blood mononuclear cells (PBMCs). MSRV load was more elevated in MS patients than in controls (p = 4.15e-7). MS women presented higher MSRV load than control women (p = 0.009) and MS men also had higher load than control men (p = 2.77e-6). Besides, women had higher levels than men, both among patients (p = 0.007) and controls (p = 1.24e-6). Concordantly, EDSS and MSSS scores were higher among female patients with an elevated MSRV load (p = 0.03 and p = 0.04, respectively). CONCLUSIONS: MSRV increases its copy number in PBMC of MS patients and particularly in women with high clinical scores. This may explain causes underlying the higher prevalence of MS in women. The association with the clinical severity calls for further investigations on MSRV load in PBMCs as a biomarker for MS. Public Library of Science 2013-01-07 /pmc/articles/PMC3538585/ /pubmed/23308264 http://dx.doi.org/10.1371/journal.pone.0053623 Text en © 2013 Garcia-Montojo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Garcia-Montojo, Marta Dominguez-Mozo, María Arias-Leal, Ana Garcia-Martinez, Ángel De las Heras, Virginia Casanova, Ignacio Faucard, Raphaël Gehin, Nadège Madeira, Alexandra Arroyo, Rafael Curtin, François Alvarez-Lafuente, Roberto Perron, Hervé The DNA Copy Number of Human Endogenous Retrovirus-W (MSRV-Type) Is Increased in Multiple Sclerosis Patients and Is Influenced by Gender and Disease Severity |
title | The DNA Copy Number of Human Endogenous Retrovirus-W (MSRV-Type) Is Increased in Multiple Sclerosis Patients and Is Influenced by Gender and Disease Severity |
title_full | The DNA Copy Number of Human Endogenous Retrovirus-W (MSRV-Type) Is Increased in Multiple Sclerosis Patients and Is Influenced by Gender and Disease Severity |
title_fullStr | The DNA Copy Number of Human Endogenous Retrovirus-W (MSRV-Type) Is Increased in Multiple Sclerosis Patients and Is Influenced by Gender and Disease Severity |
title_full_unstemmed | The DNA Copy Number of Human Endogenous Retrovirus-W (MSRV-Type) Is Increased in Multiple Sclerosis Patients and Is Influenced by Gender and Disease Severity |
title_short | The DNA Copy Number of Human Endogenous Retrovirus-W (MSRV-Type) Is Increased in Multiple Sclerosis Patients and Is Influenced by Gender and Disease Severity |
title_sort | dna copy number of human endogenous retrovirus-w (msrv-type) is increased in multiple sclerosis patients and is influenced by gender and disease severity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538585/ https://www.ncbi.nlm.nih.gov/pubmed/23308264 http://dx.doi.org/10.1371/journal.pone.0053623 |
work_keys_str_mv | AT garciamontojomarta thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT dominguezmozomaria thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT ariaslealana thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT garciamartinezangel thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT delasherasvirginia thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT casanovaignacio thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT faucardraphael thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT gehinnadege thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT madeiraalexandra thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT arroyorafael thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT curtinfrancois thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT alvarezlafuenteroberto thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT perronherve thednacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT garciamontojomarta dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT dominguezmozomaria dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT ariaslealana dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT garciamartinezangel dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT delasherasvirginia dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT casanovaignacio dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT faucardraphael dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT gehinnadege dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT madeiraalexandra dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT arroyorafael dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT curtinfrancois dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT alvarezlafuenteroberto dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity AT perronherve dnacopynumberofhumanendogenousretroviruswmsrvtypeisincreasedinmultiplesclerosispatientsandisinfluencedbygenderanddiseaseseverity |