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Standard Addition Quantitative Real-Time PCR (SAQPCR): A Novel Approach for Determination of Transgene Copy Number Avoiding PCR Efficiency Estimation

Quantitative real-time polymerase chain reaction (qPCR) has been previously applied to estimate transgene copy number in transgenic plants. However, the results can be erroneous owing to inaccurate estimation of PCR efficiency. Here, a novel qPCR approach, named standard addition qPCR (SAQPCR), was...

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Detalles Bibliográficos
Autores principales:	Huang, Yuji, Yin, Xueren, Zhu, Changqing, Wang, Weiwei, Grierson, Donald, Xu, Changjie, Chen, Kunsong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538589/ https://www.ncbi.nlm.nih.gov/pubmed/23308234 http://dx.doi.org/10.1371/journal.pone.0053489

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