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Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations
Adipose-derived stem cells (ASCs) are believed to have potential use for treating many illnesses. Most cells, including ASCs, are generally cultured in medium containing fetal bovine serum (FBS). However, FBS, which could induce an immune response or infection, is not recommended for clinical applic...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539127/ https://www.ncbi.nlm.nih.gov/pubmed/23271183 http://dx.doi.org/10.4142/jvs.2012.13.4.413 |
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author | Bahn, Jae-Jun Chung, Jin-Young Im, Wooseok Kim, Manho Kim, Sae Hoon |
author_facet | Bahn, Jae-Jun Chung, Jin-Young Im, Wooseok Kim, Manho Kim, Sae Hoon |
author_sort | Bahn, Jae-Jun |
collection | PubMed |
description | Adipose-derived stem cells (ASCs) are believed to have potential use for treating many illnesses. Most cells, including ASCs, are generally cultured in medium containing fetal bovine serum (FBS). However, FBS, which could induce an immune response or infection, is not recommended for clinical applications. In the present study, we evaluated the morphology, proliferation rate, and characterization of rabbit ASCs grown in medium containing autologous serum (AS) and compared these cells to ones cultured with FBS. Morphological changes were monitored by microscopy and flow cytometry. Proliferation rates were assessed with cell counting and ASC phenotypes were characterized by flow cytometry using representative surface markers (CD44 and CD45). Expression of epidermal growth factor, brain-derived neurotrophic factor, and vascular endothelial growth factor was measured by reverse transcription-polymerase chain reaction. Results of our study showed that ASCs had a greater expansion rate in AS without developing morphological heterogeneity than cells grown in FBS. AS-cultured ASCs expressed representative growth factors, CD44 but not CD45, similar to cells cultured in FBS. Expression levels of some growth factors were different between AS and FBS. In conclusion, our findings indicated that AS could potentially be used as a culture medium supplement for the expansion of autologous ASCs. |
format | Online Article Text |
id | pubmed-3539127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35391272013-01-15 Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations Bahn, Jae-Jun Chung, Jin-Young Im, Wooseok Kim, Manho Kim, Sae Hoon J Vet Sci Original Article Adipose-derived stem cells (ASCs) are believed to have potential use for treating many illnesses. Most cells, including ASCs, are generally cultured in medium containing fetal bovine serum (FBS). However, FBS, which could induce an immune response or infection, is not recommended for clinical applications. In the present study, we evaluated the morphology, proliferation rate, and characterization of rabbit ASCs grown in medium containing autologous serum (AS) and compared these cells to ones cultured with FBS. Morphological changes were monitored by microscopy and flow cytometry. Proliferation rates were assessed with cell counting and ASC phenotypes were characterized by flow cytometry using representative surface markers (CD44 and CD45). Expression of epidermal growth factor, brain-derived neurotrophic factor, and vascular endothelial growth factor was measured by reverse transcription-polymerase chain reaction. Results of our study showed that ASCs had a greater expansion rate in AS without developing morphological heterogeneity than cells grown in FBS. AS-cultured ASCs expressed representative growth factors, CD44 but not CD45, similar to cells cultured in FBS. Expression levels of some growth factors were different between AS and FBS. In conclusion, our findings indicated that AS could potentially be used as a culture medium supplement for the expansion of autologous ASCs. The Korean Society of Veterinary Science 2012-12 2012-12-20 /pmc/articles/PMC3539127/ /pubmed/23271183 http://dx.doi.org/10.4142/jvs.2012.13.4.413 Text en Copyright © 2012 The Korean Society of Veterinary Science http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Bahn, Jae-Jun Chung, Jin-Young Im, Wooseok Kim, Manho Kim, Sae Hoon Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations |
title | Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations |
title_full | Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations |
title_fullStr | Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations |
title_full_unstemmed | Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations |
title_short | Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations |
title_sort | suitability of autologous serum for expanding rabbit adipose-derived stem cell populations |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539127/ https://www.ncbi.nlm.nih.gov/pubmed/23271183 http://dx.doi.org/10.4142/jvs.2012.13.4.413 |
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