Development of monoclonal antibodies against the abnormal prion protein isoform (PrP(res)) associated with chronic wasting disease (CWD)

Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP(res)) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of...

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Detalles Bibliográficos
Autores principales: Jeong, Hyun-Jeong, Lee, Nak-Hyung, Lee, Joong-Bok, Park, Seung-Yong, Song, Chang-Seon, Seo, Kun-Ho, Kim, Dong-Woon, Kim, Yong-Sun, Choi, In-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539130/
https://www.ncbi.nlm.nih.gov/pubmed/23271186
http://dx.doi.org/10.4142/jvs.2012.13.4.429
Descripción
Sumario:Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP(res)) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.

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