Development of monoclonal antibodies against the abnormal prion protein isoform (PrP(res)) associated with chronic wasting disease (CWD)

Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP(res)) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of...

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Autores principales: Jeong, Hyun-Jeong, Lee, Nak-Hyung, Lee, Joong-Bok, Park, Seung-Yong, Song, Chang-Seon, Seo, Kun-Ho, Kim, Dong-Woon, Kim, Yong-Sun, Choi, In-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539130/
https://www.ncbi.nlm.nih.gov/pubmed/23271186
http://dx.doi.org/10.4142/jvs.2012.13.4.429
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author Jeong, Hyun-Jeong
Lee, Nak-Hyung
Lee, Joong-Bok
Park, Seung-Yong
Song, Chang-Seon
Seo, Kun-Ho
Kim, Dong-Woon
Kim, Yong-Sun
Choi, In-Soo
author_facet Jeong, Hyun-Jeong
Lee, Nak-Hyung
Lee, Joong-Bok
Park, Seung-Yong
Song, Chang-Seon
Seo, Kun-Ho
Kim, Dong-Woon
Kim, Yong-Sun
Choi, In-Soo
author_sort Jeong, Hyun-Jeong
collection PubMed
description Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP(res)) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.
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spelling pubmed-35391302013-01-15 Development of monoclonal antibodies against the abnormal prion protein isoform (PrP(res)) associated with chronic wasting disease (CWD) Jeong, Hyun-Jeong Lee, Nak-Hyung Lee, Joong-Bok Park, Seung-Yong Song, Chang-Seon Seo, Kun-Ho Kim, Dong-Woon Kim, Yong-Sun Choi, In-Soo J Vet Sci Short Communication Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP(res)) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD. The Korean Society of Veterinary Science 2012-12 2012-12-20 /pmc/articles/PMC3539130/ /pubmed/23271186 http://dx.doi.org/10.4142/jvs.2012.13.4.429 Text en Copyright © 2012 The Korean Society of Veterinary Science http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Jeong, Hyun-Jeong
Lee, Nak-Hyung
Lee, Joong-Bok
Park, Seung-Yong
Song, Chang-Seon
Seo, Kun-Ho
Kim, Dong-Woon
Kim, Yong-Sun
Choi, In-Soo
Development of monoclonal antibodies against the abnormal prion protein isoform (PrP(res)) associated with chronic wasting disease (CWD)
title Development of monoclonal antibodies against the abnormal prion protein isoform (PrP(res)) associated with chronic wasting disease (CWD)
title_full Development of monoclonal antibodies against the abnormal prion protein isoform (PrP(res)) associated with chronic wasting disease (CWD)
title_fullStr Development of monoclonal antibodies against the abnormal prion protein isoform (PrP(res)) associated with chronic wasting disease (CWD)
title_full_unstemmed Development of monoclonal antibodies against the abnormal prion protein isoform (PrP(res)) associated with chronic wasting disease (CWD)
title_short Development of monoclonal antibodies against the abnormal prion protein isoform (PrP(res)) associated with chronic wasting disease (CWD)
title_sort development of monoclonal antibodies against the abnormal prion protein isoform (prp(res)) associated with chronic wasting disease (cwd)
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539130/
https://www.ncbi.nlm.nih.gov/pubmed/23271186
http://dx.doi.org/10.4142/jvs.2012.13.4.429
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