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Effect of signal intensity and camera quantization on laser speckle contrast analysis
Laser speckle contrast analysis (LASCA) is limited to being a qualitative method for the measurement of blood flow and tissue perfusion as it is sensitive to the measurement configuration. The signal intensity is one of the parameters that can affect the contrast values due to the quantization of th...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539185/ https://www.ncbi.nlm.nih.gov/pubmed/23304650 http://dx.doi.org/10.1364/BOE.4.000089 |
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author | Song, Lipei Elson, Daniel S. |
author_facet | Song, Lipei Elson, Daniel S. |
author_sort | Song, Lipei |
collection | PubMed |
description | Laser speckle contrast analysis (LASCA) is limited to being a qualitative method for the measurement of blood flow and tissue perfusion as it is sensitive to the measurement configuration. The signal intensity is one of the parameters that can affect the contrast values due to the quantization of the signals by the camera and analog-to-digital converter (ADC). In this paper we deduce the theoretical relationship between signal intensity and contrast values based on the probability density function (PDF) of the speckle pattern and simplify it to a rational function. A simple method to correct this contrast error is suggested. The experimental results demonstrate that this relationship can effectively compensate the bias in contrast values induced by the quantized signal intensity and correct for bias induced by signal intensity variations across the field of view. |
format | Online Article Text |
id | pubmed-3539185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-35391852013-01-09 Effect of signal intensity and camera quantization on laser speckle contrast analysis Song, Lipei Elson, Daniel S. Biomed Opt Express Speckle Imaging and Diagnostics Laser speckle contrast analysis (LASCA) is limited to being a qualitative method for the measurement of blood flow and tissue perfusion as it is sensitive to the measurement configuration. The signal intensity is one of the parameters that can affect the contrast values due to the quantization of the signals by the camera and analog-to-digital converter (ADC). In this paper we deduce the theoretical relationship between signal intensity and contrast values based on the probability density function (PDF) of the speckle pattern and simplify it to a rational function. A simple method to correct this contrast error is suggested. The experimental results demonstrate that this relationship can effectively compensate the bias in contrast values induced by the quantized signal intensity and correct for bias induced by signal intensity variations across the field of view. Optical Society of America 2012-12-13 /pmc/articles/PMC3539185/ /pubmed/23304650 http://dx.doi.org/10.1364/BOE.4.000089 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Speckle Imaging and Diagnostics Song, Lipei Elson, Daniel S. Effect of signal intensity and camera quantization on laser speckle contrast analysis |
title | Effect of signal intensity and camera quantization on laser speckle contrast analysis |
title_full | Effect of signal intensity and camera quantization on laser speckle contrast analysis |
title_fullStr | Effect of signal intensity and camera quantization on laser speckle contrast analysis |
title_full_unstemmed | Effect of signal intensity and camera quantization on laser speckle contrast analysis |
title_short | Effect of signal intensity and camera quantization on laser speckle contrast analysis |
title_sort | effect of signal intensity and camera quantization on laser speckle contrast analysis |
topic | Speckle Imaging and Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539185/ https://www.ncbi.nlm.nih.gov/pubmed/23304650 http://dx.doi.org/10.1364/BOE.4.000089 |
work_keys_str_mv | AT songlipei effectofsignalintensityandcameraquantizationonlaserspecklecontrastanalysis AT elsondaniels effectofsignalintensityandcameraquantizationonlaserspecklecontrastanalysis |