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Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells

We show that the spectral phasor approach of the fluorescent dye Pyronin Y (PY) can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells. We applied spectral phasors to isolate intracellular RNA species with similar spectral properties. We identified at least 4...

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Detalles Bibliográficos
Autores principales:	Andrews, Laura M., Jones, Mark R., Digman, Michelle A., Gratton, Enrico
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Optical Society of America 2012
Materias:	Spectroscopic Diagnostics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539199/ https://www.ncbi.nlm.nih.gov/pubmed/23304656 http://dx.doi.org/10.1364/BOE.4.000171

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