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Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer

BACKGROUND: The broad spectrum of oxidative damage DNA biomarkers: urinary excretion of 8-oxodG (8-oxo-7,8-dihydro-2′-deoxyguanosine), 8-oxoGua (8-oxo-7,8-dihydroguanine) as well as the level of oxidative damage DNA in leukocytes, was analyzed in cancer patients and healthy subjects. MATERIAL/METHOD...

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Autores principales: Roszkowski, Krzysztof, Jozwicki, Wojciech, Blaszczyk, Piotr, Mucha-Malecka, Anna, Siomek, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539537/
https://www.ncbi.nlm.nih.gov/pubmed/21629187
http://dx.doi.org/10.12659/MSM.881805
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author Roszkowski, Krzysztof
Jozwicki, Wojciech
Blaszczyk, Piotr
Mucha-Malecka, Anna
Siomek, Agnieszka
author_facet Roszkowski, Krzysztof
Jozwicki, Wojciech
Blaszczyk, Piotr
Mucha-Malecka, Anna
Siomek, Agnieszka
author_sort Roszkowski, Krzysztof
collection PubMed
description BACKGROUND: The broad spectrum of oxidative damage DNA biomarkers: urinary excretion of 8-oxodG (8-oxo-7,8-dihydro-2′-deoxyguanosine), 8-oxoGua (8-oxo-7,8-dihydroguanine) as well as the level of oxidative damage DNA in leukocytes, was analyzed in cancer patients and healthy subjects. MATERIAL/METHODS: 222 cancer patients and 134 healthy volunteers were included in the analysis, using methodologies which involve HPLC (high-performance liquid chromatography) prepurification followed by gas chromatography with isotope dilution mass spectrometry detection and HPLC/EC. RESULTS: For the whole patient population (n=222) the median values of 8-oxoGua and 8-oxodG in urine samples were 12.44 (interquartile range: 8.14–20.33) [nmol/24 hr] and 6.05 (3.12–15.38) [nmol/24 hr], respectively. The median values of 8-oxoGua and 8-oxodG in urine samples of the control group (n=85) were 7.7 (4.65–10.15) [nmol/24 hr] and 2.2 (1.7–2.8) [nmol/24 hr], respectively. The level of 8-oxodG in DNA isolated from leukocytes of the patient population (n=179) and of the control group (n=134) was 4.93 (3.46–9.27) per 10’6 dG and 4.46 (3.82–5.31) per 10’6 dG, respectively. CONCLUSIONS: The results suggest that oxidative stress in cancer patients, demonstrated by augmented amounts of these modifications in urine, could be typical not only for affected tissue but also for other tissues and even the whole organism. An assay that enables the determination of levels of basic markers of oxidative stress might be applied in clinical practice as an additional, helpful marker to diagnose cancer.
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spelling pubmed-35395372013-04-24 Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer Roszkowski, Krzysztof Jozwicki, Wojciech Blaszczyk, Piotr Mucha-Malecka, Anna Siomek, Agnieszka Med Sci Monit Clinical Research BACKGROUND: The broad spectrum of oxidative damage DNA biomarkers: urinary excretion of 8-oxodG (8-oxo-7,8-dihydro-2′-deoxyguanosine), 8-oxoGua (8-oxo-7,8-dihydroguanine) as well as the level of oxidative damage DNA in leukocytes, was analyzed in cancer patients and healthy subjects. MATERIAL/METHODS: 222 cancer patients and 134 healthy volunteers were included in the analysis, using methodologies which involve HPLC (high-performance liquid chromatography) prepurification followed by gas chromatography with isotope dilution mass spectrometry detection and HPLC/EC. RESULTS: For the whole patient population (n=222) the median values of 8-oxoGua and 8-oxodG in urine samples were 12.44 (interquartile range: 8.14–20.33) [nmol/24 hr] and 6.05 (3.12–15.38) [nmol/24 hr], respectively. The median values of 8-oxoGua and 8-oxodG in urine samples of the control group (n=85) were 7.7 (4.65–10.15) [nmol/24 hr] and 2.2 (1.7–2.8) [nmol/24 hr], respectively. The level of 8-oxodG in DNA isolated from leukocytes of the patient population (n=179) and of the control group (n=134) was 4.93 (3.46–9.27) per 10’6 dG and 4.46 (3.82–5.31) per 10’6 dG, respectively. CONCLUSIONS: The results suggest that oxidative stress in cancer patients, demonstrated by augmented amounts of these modifications in urine, could be typical not only for affected tissue but also for other tissues and even the whole organism. An assay that enables the determination of levels of basic markers of oxidative stress might be applied in clinical practice as an additional, helpful marker to diagnose cancer. International Scientific Literature, Inc. 2011-06-01 /pmc/articles/PMC3539537/ /pubmed/21629187 http://dx.doi.org/10.12659/MSM.881805 Text en © Med Sci Monit, 2011 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
spellingShingle Clinical Research
Roszkowski, Krzysztof
Jozwicki, Wojciech
Blaszczyk, Piotr
Mucha-Malecka, Anna
Siomek, Agnieszka
Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer
title Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer
title_full Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer
title_fullStr Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer
title_full_unstemmed Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer
title_short Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer
title_sort oxidative damage dna: 8-oxogua and 8-oxodg as molecular markers of cancer
topic Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539537/
https://www.ncbi.nlm.nih.gov/pubmed/21629187
http://dx.doi.org/10.12659/MSM.881805
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