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The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps
BACKGROUND: To investigate the expression levels of importin13 (IPO13), c-kit, CD146, telomerase, caspase-3, bcl-2 and bax in endometrial polyps (EPs). MATERIAL/METHODS: We detected the mRNA expression levels of IPO13, c-kit, bcl-2 and bax in endometrial polyps (EPs) using real-time PCR. We detected...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539613/ https://www.ncbi.nlm.nih.gov/pubmed/21804459 http://dx.doi.org/10.12659/MSM.881901 |
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author | Hu, JianGuo Yuan, Rui |
author_facet | Hu, JianGuo Yuan, Rui |
author_sort | Hu, JianGuo |
collection | PubMed |
description | BACKGROUND: To investigate the expression levels of importin13 (IPO13), c-kit, CD146, telomerase, caspase-3, bcl-2 and bax in endometrial polyps (EPs). MATERIAL/METHODS: We detected the mRNA expression levels of IPO13, c-kit, bcl-2 and bax in endometrial polyps (EPs) using real-time PCR. We detected the protein expression levels of IPO13, telomerase, CD146, caspase-3, bcl-2 and bax in EPs using S-P (Streptavidin-Peroxidase) immunohistochemistry. Western blotting was performed to determine the levels of importin13 and bcl-2 proteins in EPs. RESULTS: The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were lower in the EP tissue compared to normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05). The expression of CD146 was decreased in the EP tissue compared to the normal endometrial tissue during the proliferation phase of the menstrual cycle (p<0.05). The expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05). CONCLUSIONS: The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were decreased; however, the expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue. These findings suggest that the development of EPs is associated with the deregulated activities of the endometrial stem/progenitor cells and the decreased apoptosis of endometrial cells, with the latter being the major factor involved in the development of EPs. |
format | Online Article Text |
id | pubmed-3539613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-35396132013-04-24 The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps Hu, JianGuo Yuan, Rui Med Sci Monit Basic Research BACKGROUND: To investigate the expression levels of importin13 (IPO13), c-kit, CD146, telomerase, caspase-3, bcl-2 and bax in endometrial polyps (EPs). MATERIAL/METHODS: We detected the mRNA expression levels of IPO13, c-kit, bcl-2 and bax in endometrial polyps (EPs) using real-time PCR. We detected the protein expression levels of IPO13, telomerase, CD146, caspase-3, bcl-2 and bax in EPs using S-P (Streptavidin-Peroxidase) immunohistochemistry. Western blotting was performed to determine the levels of importin13 and bcl-2 proteins in EPs. RESULTS: The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were lower in the EP tissue compared to normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05). The expression of CD146 was decreased in the EP tissue compared to the normal endometrial tissue during the proliferation phase of the menstrual cycle (p<0.05). The expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05). CONCLUSIONS: The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were decreased; however, the expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue. These findings suggest that the development of EPs is associated with the deregulated activities of the endometrial stem/progenitor cells and the decreased apoptosis of endometrial cells, with the latter being the major factor involved in the development of EPs. International Scientific Literature, Inc. 2011-08-01 /pmc/articles/PMC3539613/ /pubmed/21804459 http://dx.doi.org/10.12659/MSM.881901 Text en © Med Sci Monit, 2011 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. |
spellingShingle | Basic Research Hu, JianGuo Yuan, Rui The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps |
title | The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps |
title_full | The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps |
title_fullStr | The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps |
title_full_unstemmed | The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps |
title_short | The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps |
title_sort | expression levels of stem cell markers importin13, c-kit, cd146, and telomerase are decreased in endometrial polyps |
topic | Basic Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539613/ https://www.ncbi.nlm.nih.gov/pubmed/21804459 http://dx.doi.org/10.12659/MSM.881901 |
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