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The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps

BACKGROUND: To investigate the expression levels of importin13 (IPO13), c-kit, CD146, telomerase, caspase-3, bcl-2 and bax in endometrial polyps (EPs). MATERIAL/METHODS: We detected the mRNA expression levels of IPO13, c-kit, bcl-2 and bax in endometrial polyps (EPs) using real-time PCR. We detected...

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Autores principales: Hu, JianGuo, Yuan, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539613/
https://www.ncbi.nlm.nih.gov/pubmed/21804459
http://dx.doi.org/10.12659/MSM.881901
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author Hu, JianGuo
Yuan, Rui
author_facet Hu, JianGuo
Yuan, Rui
author_sort Hu, JianGuo
collection PubMed
description BACKGROUND: To investigate the expression levels of importin13 (IPO13), c-kit, CD146, telomerase, caspase-3, bcl-2 and bax in endometrial polyps (EPs). MATERIAL/METHODS: We detected the mRNA expression levels of IPO13, c-kit, bcl-2 and bax in endometrial polyps (EPs) using real-time PCR. We detected the protein expression levels of IPO13, telomerase, CD146, caspase-3, bcl-2 and bax in EPs using S-P (Streptavidin-Peroxidase) immunohistochemistry. Western blotting was performed to determine the levels of importin13 and bcl-2 proteins in EPs. RESULTS: The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were lower in the EP tissue compared to normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05). The expression of CD146 was decreased in the EP tissue compared to the normal endometrial tissue during the proliferation phase of the menstrual cycle (p<0.05). The expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05). CONCLUSIONS: The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were decreased; however, the expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue. These findings suggest that the development of EPs is associated with the deregulated activities of the endometrial stem/progenitor cells and the decreased apoptosis of endometrial cells, with the latter being the major factor involved in the development of EPs.
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spelling pubmed-35396132013-04-24 The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps Hu, JianGuo Yuan, Rui Med Sci Monit Basic Research BACKGROUND: To investigate the expression levels of importin13 (IPO13), c-kit, CD146, telomerase, caspase-3, bcl-2 and bax in endometrial polyps (EPs). MATERIAL/METHODS: We detected the mRNA expression levels of IPO13, c-kit, bcl-2 and bax in endometrial polyps (EPs) using real-time PCR. We detected the protein expression levels of IPO13, telomerase, CD146, caspase-3, bcl-2 and bax in EPs using S-P (Streptavidin-Peroxidase) immunohistochemistry. Western blotting was performed to determine the levels of importin13 and bcl-2 proteins in EPs. RESULTS: The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were lower in the EP tissue compared to normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05). The expression of CD146 was decreased in the EP tissue compared to the normal endometrial tissue during the proliferation phase of the menstrual cycle (p<0.05). The expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05). CONCLUSIONS: The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were decreased; however, the expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue. These findings suggest that the development of EPs is associated with the deregulated activities of the endometrial stem/progenitor cells and the decreased apoptosis of endometrial cells, with the latter being the major factor involved in the development of EPs. International Scientific Literature, Inc. 2011-08-01 /pmc/articles/PMC3539613/ /pubmed/21804459 http://dx.doi.org/10.12659/MSM.881901 Text en © Med Sci Monit, 2011 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
spellingShingle Basic Research
Hu, JianGuo
Yuan, Rui
The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps
title The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps
title_full The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps
title_fullStr The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps
title_full_unstemmed The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps
title_short The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps
title_sort expression levels of stem cell markers importin13, c-kit, cd146, and telomerase are decreased in endometrial polyps
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539613/
https://www.ncbi.nlm.nih.gov/pubmed/21804459
http://dx.doi.org/10.12659/MSM.881901
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