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Protective effect of chitosan oligosaccharide lactate against DNA double-strand breaks induced by a model methacrylate dental adhesive

BACKGROUND: Monomers of methacrylates used in restorative dentistry have been recently reported to induce DNA double-strand breaks (DSBs) in human gingival fibroblasts (HGFs) in vitro. Because such monomers may penetrate the pulp and oral cavity due to the incompleteness of polymerization and polyme...

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Autores principales: Szczepanska, Joanna, Pawlowska, Elzbieta, Synowiec, Ewelina, Czarny, Piotr, Rekas, Marek, Blasiak, Janusz, Szaflik, Jacek Pawel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539618/
https://www.ncbi.nlm.nih.gov/pubmed/21804456
http://dx.doi.org/10.12659/MSM.881898
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author Szczepanska, Joanna
Pawlowska, Elzbieta
Synowiec, Ewelina
Czarny, Piotr
Rekas, Marek
Blasiak, Janusz
Szaflik, Jacek Pawel
author_facet Szczepanska, Joanna
Pawlowska, Elzbieta
Synowiec, Ewelina
Czarny, Piotr
Rekas, Marek
Blasiak, Janusz
Szaflik, Jacek Pawel
author_sort Szczepanska, Joanna
collection PubMed
description BACKGROUND: Monomers of methacrylates used in restorative dentistry have been recently reported to induce DNA double-strand breaks (DSBs) in human gingival fibroblasts (HGFs) in vitro. Because such monomers may penetrate the pulp and oral cavity due to the incompleteness of polymerization and polymer degradation, they may induce a similar effect in vivo. DSBs are the most serious type of DNA damage and if misrepaired or not repaired may lead to mutation, cancer transformation and cell death. Therefore, the protection against DSBs induced by methacrylate monomers released from dental restorations is imperative. MATERIAL/METHODS: We examined the protective action of chitosan oligosaccharide lactate (ChOL) against cytotoxic and genotoxic effects induced by monomers of the model adhesive consisting of 55% bisphenol A-diglycidyl dimethacrylate (Bis-GMA) and 45% 2-hydroxyethyl methacrylate (HEMA). We evaluated the extent of DSBs by the neutral comet assay and the phosphorylation of the H2AX histone test. RESULTS: ChOL increased the viability of HGFs exposed to Bis-GMA/HEMA as assessed by flow cytometry. ChOL decreased the extent of DSBs induced by Bis-GMA/HEMA as evaluated by neutral comet assay and phosphorylation of the H2AX histone. ChOL did not change mechanical properties of the model adhesive, as checked by the shear bond test. Scanning electron microscopy revealed a better sealing of the dentinal microtubules in the presence of ChOL, which may protect pulp cells against the harmful action of the monomers. CONCLUSIONS: ChOL can be considered as an additive to methacrylate-based dental materials to prevent DSBs induction, but further studies are needed on its formulation with the methacrylates.
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spelling pubmed-35396182013-04-24 Protective effect of chitosan oligosaccharide lactate against DNA double-strand breaks induced by a model methacrylate dental adhesive Szczepanska, Joanna Pawlowska, Elzbieta Synowiec, Ewelina Czarny, Piotr Rekas, Marek Blasiak, Janusz Szaflik, Jacek Pawel Med Sci Monit Basic Research BACKGROUND: Monomers of methacrylates used in restorative dentistry have been recently reported to induce DNA double-strand breaks (DSBs) in human gingival fibroblasts (HGFs) in vitro. Because such monomers may penetrate the pulp and oral cavity due to the incompleteness of polymerization and polymer degradation, they may induce a similar effect in vivo. DSBs are the most serious type of DNA damage and if misrepaired or not repaired may lead to mutation, cancer transformation and cell death. Therefore, the protection against DSBs induced by methacrylate monomers released from dental restorations is imperative. MATERIAL/METHODS: We examined the protective action of chitosan oligosaccharide lactate (ChOL) against cytotoxic and genotoxic effects induced by monomers of the model adhesive consisting of 55% bisphenol A-diglycidyl dimethacrylate (Bis-GMA) and 45% 2-hydroxyethyl methacrylate (HEMA). We evaluated the extent of DSBs by the neutral comet assay and the phosphorylation of the H2AX histone test. RESULTS: ChOL increased the viability of HGFs exposed to Bis-GMA/HEMA as assessed by flow cytometry. ChOL decreased the extent of DSBs induced by Bis-GMA/HEMA as evaluated by neutral comet assay and phosphorylation of the H2AX histone. ChOL did not change mechanical properties of the model adhesive, as checked by the shear bond test. Scanning electron microscopy revealed a better sealing of the dentinal microtubules in the presence of ChOL, which may protect pulp cells against the harmful action of the monomers. CONCLUSIONS: ChOL can be considered as an additive to methacrylate-based dental materials to prevent DSBs induction, but further studies are needed on its formulation with the methacrylates. International Scientific Literature, Inc. 2011-08-01 /pmc/articles/PMC3539618/ /pubmed/21804456 http://dx.doi.org/10.12659/MSM.881898 Text en © Med Sci Monit, 2011 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
spellingShingle Basic Research
Szczepanska, Joanna
Pawlowska, Elzbieta
Synowiec, Ewelina
Czarny, Piotr
Rekas, Marek
Blasiak, Janusz
Szaflik, Jacek Pawel
Protective effect of chitosan oligosaccharide lactate against DNA double-strand breaks induced by a model methacrylate dental adhesive
title Protective effect of chitosan oligosaccharide lactate against DNA double-strand breaks induced by a model methacrylate dental adhesive
title_full Protective effect of chitosan oligosaccharide lactate against DNA double-strand breaks induced by a model methacrylate dental adhesive
title_fullStr Protective effect of chitosan oligosaccharide lactate against DNA double-strand breaks induced by a model methacrylate dental adhesive
title_full_unstemmed Protective effect of chitosan oligosaccharide lactate against DNA double-strand breaks induced by a model methacrylate dental adhesive
title_short Protective effect of chitosan oligosaccharide lactate against DNA double-strand breaks induced by a model methacrylate dental adhesive
title_sort protective effect of chitosan oligosaccharide lactate against dna double-strand breaks induced by a model methacrylate dental adhesive
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539618/
https://www.ncbi.nlm.nih.gov/pubmed/21804456
http://dx.doi.org/10.12659/MSM.881898
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