Cargando…

Administration of obestatin accelerates the healing of chronic gastric ulcers in rats

BACKGROUND: Previous studies have shown that administration of obestatin exhibits a protective effect in the pancreas, attenuating the development of acute pancreatitis. The aim of the present study was to investigate the influence of obestatin administration on the healing of chronic gastric ulcers...

Descripción completa

Detalles Bibliográficos
Autores principales: Dembiński, Artur, Warzecha, Zygmunt, Ceranowicz, Piotr, Cieszkowski, Jakub, Dembiński, Marcin, Ptak-Belowska, Agata, Kuwahara, Atsukasu, Kato, Ikuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539620/
https://www.ncbi.nlm.nih.gov/pubmed/21804455
http://dx.doi.org/10.12659/MSM.881897
_version_ 1782255127034331136
author Dembiński, Artur
Warzecha, Zygmunt
Ceranowicz, Piotr
Cieszkowski, Jakub
Dembiński, Marcin
Ptak-Belowska, Agata
Kuwahara, Atsukasu
Kato, Ikuo
author_facet Dembiński, Artur
Warzecha, Zygmunt
Ceranowicz, Piotr
Cieszkowski, Jakub
Dembiński, Marcin
Ptak-Belowska, Agata
Kuwahara, Atsukasu
Kato, Ikuo
author_sort Dembiński, Artur
collection PubMed
description BACKGROUND: Previous studies have shown that administration of obestatin exhibits a protective effect in the pancreas, attenuating the development of acute pancreatitis. The aim of the present study was to investigate the influence of obestatin administration on the healing of chronic gastric ulcers. MATERIAL/METHODS: Chronic gastric ulcers were induced in rats by 100% acetic acid applied to the serosal surface of the gastric wall. Obestatin was given twice a day intraperitoneally at the dose of 4, 8 or 16 nmol/kg/dose for 6 days. Six days after induction of ulcers, rats were anesthetized and the stomach was exposed for measurement of gastric blood flow and ulcer area. Biopsy samples from the gastric mucosa were taken for determination of mucosal DNA synthesis and for measurement of gastric expression of mRNA for interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). RESULTS: Induction of gastric ulcers alone increased mucosal blood flow and tissue expression of mRNA for TNF-α and IL-1β, whereas gastric mucosal DNA synthesis was reduced. In rats with gastric ulcers, administration of obestatin increased gastric mucosal blood flow, accelerated the healing rate of these ulcers and partly reversed the gastric ulcer-induced reduction in gastric mucosal DNA synthesis. These results were associated with a reduction in gastric mucosal expression of pro-inflammatory IL-1β and TNF-α. CONCLUSIONS: Treatment with obestatin increases gastric mucosal blood flow and cell proliferation, leading to acceleration of healing of gastric ulcers. These effects are associated with a reduction in mucosal expression of pro-inflammatory IL-1β and TNF-α.
format Online
Article
Text
id pubmed-3539620
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher International Scientific Literature, Inc.
record_format MEDLINE/PubMed
spelling pubmed-35396202013-04-24 Administration of obestatin accelerates the healing of chronic gastric ulcers in rats Dembiński, Artur Warzecha, Zygmunt Ceranowicz, Piotr Cieszkowski, Jakub Dembiński, Marcin Ptak-Belowska, Agata Kuwahara, Atsukasu Kato, Ikuo Med Sci Monit Basic Research BACKGROUND: Previous studies have shown that administration of obestatin exhibits a protective effect in the pancreas, attenuating the development of acute pancreatitis. The aim of the present study was to investigate the influence of obestatin administration on the healing of chronic gastric ulcers. MATERIAL/METHODS: Chronic gastric ulcers were induced in rats by 100% acetic acid applied to the serosal surface of the gastric wall. Obestatin was given twice a day intraperitoneally at the dose of 4, 8 or 16 nmol/kg/dose for 6 days. Six days after induction of ulcers, rats were anesthetized and the stomach was exposed for measurement of gastric blood flow and ulcer area. Biopsy samples from the gastric mucosa were taken for determination of mucosal DNA synthesis and for measurement of gastric expression of mRNA for interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). RESULTS: Induction of gastric ulcers alone increased mucosal blood flow and tissue expression of mRNA for TNF-α and IL-1β, whereas gastric mucosal DNA synthesis was reduced. In rats with gastric ulcers, administration of obestatin increased gastric mucosal blood flow, accelerated the healing rate of these ulcers and partly reversed the gastric ulcer-induced reduction in gastric mucosal DNA synthesis. These results were associated with a reduction in gastric mucosal expression of pro-inflammatory IL-1β and TNF-α. CONCLUSIONS: Treatment with obestatin increases gastric mucosal blood flow and cell proliferation, leading to acceleration of healing of gastric ulcers. These effects are associated with a reduction in mucosal expression of pro-inflammatory IL-1β and TNF-α. International Scientific Literature, Inc. 2011-08-01 /pmc/articles/PMC3539620/ /pubmed/21804455 http://dx.doi.org/10.12659/MSM.881897 Text en © Med Sci Monit, 2011 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
spellingShingle Basic Research
Dembiński, Artur
Warzecha, Zygmunt
Ceranowicz, Piotr
Cieszkowski, Jakub
Dembiński, Marcin
Ptak-Belowska, Agata
Kuwahara, Atsukasu
Kato, Ikuo
Administration of obestatin accelerates the healing of chronic gastric ulcers in rats
title Administration of obestatin accelerates the healing of chronic gastric ulcers in rats
title_full Administration of obestatin accelerates the healing of chronic gastric ulcers in rats
title_fullStr Administration of obestatin accelerates the healing of chronic gastric ulcers in rats
title_full_unstemmed Administration of obestatin accelerates the healing of chronic gastric ulcers in rats
title_short Administration of obestatin accelerates the healing of chronic gastric ulcers in rats
title_sort administration of obestatin accelerates the healing of chronic gastric ulcers in rats
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539620/
https://www.ncbi.nlm.nih.gov/pubmed/21804455
http://dx.doi.org/10.12659/MSM.881897
work_keys_str_mv AT dembinskiartur administrationofobestatinacceleratesthehealingofchronicgastriculcersinrats
AT warzechazygmunt administrationofobestatinacceleratesthehealingofchronicgastriculcersinrats
AT ceranowiczpiotr administrationofobestatinacceleratesthehealingofchronicgastriculcersinrats
AT cieszkowskijakub administrationofobestatinacceleratesthehealingofchronicgastriculcersinrats
AT dembinskimarcin administrationofobestatinacceleratesthehealingofchronicgastriculcersinrats
AT ptakbelowskaagata administrationofobestatinacceleratesthehealingofchronicgastriculcersinrats
AT kuwaharaatsukasu administrationofobestatinacceleratesthehealingofchronicgastriculcersinrats
AT katoikuo administrationofobestatinacceleratesthehealingofchronicgastriculcersinrats