Cargando…
Secreted Factors from Bone Marrow Stromal Cells Upregulate IL-10 and Reverse Acute Kidney Injury
Acute kidney injury is a devastating syndrome that afflicts over 2,000,000 people in the US per year, with an associated mortality of greater than 70% in severe cases. Unfortunately, standard-of-care treatments are not sufficient for modifying the course of disease. Many groups have explored the use...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539665/ https://www.ncbi.nlm.nih.gov/pubmed/23319959 http://dx.doi.org/10.1155/2012/392050 |
_version_ | 1782255132184936448 |
---|---|
author | Milwid, Jack M. Ichimura, Takaharu Li, Matthew Jiao, Yunxin Lee, Jungwoo Yarmush, Joshua S. Parekkadan, Biju Tilles, Arno W. Bonventre, Joseph V. Yarmush, Martin L. |
author_facet | Milwid, Jack M. Ichimura, Takaharu Li, Matthew Jiao, Yunxin Lee, Jungwoo Yarmush, Joshua S. Parekkadan, Biju Tilles, Arno W. Bonventre, Joseph V. Yarmush, Martin L. |
author_sort | Milwid, Jack M. |
collection | PubMed |
description | Acute kidney injury is a devastating syndrome that afflicts over 2,000,000 people in the US per year, with an associated mortality of greater than 70% in severe cases. Unfortunately, standard-of-care treatments are not sufficient for modifying the course of disease. Many groups have explored the use of bone marrow stromal cells (BMSCs) for the treatment of AKI because BMSCs have been shown to possess unique anti-inflammatory, cytoprotective, and regenerative properties in vitro and in vivo. It is yet unresolved whether the primary mechanisms controlling BMSC therapy in AKI depend on direct cell infusion, or whether BMSC-secreted factors alone are sufficient for mitigating the injury. Here we show that BMSC-secreted factors are capable of providing a survival benefit to rats subjected to cisplatin-induced AKI. We observed that when BMSC-conditioned medium (BMSC-CM) is administered intravenously, it prevents tubular apoptosis and necrosis and ameliorates AKI. In addition, we observed that BMSC-CM causes IL-10 upregulation in treated animals, which is important to animal survival and protection of the kidney. In all, these results demonstrate that BMSC-secreted factors are capable of providing support without cell transplantation, and the IL-10 increase seen in BMSC-CM-treated animals correlates with attenuation of severe AKI. |
format | Online Article Text |
id | pubmed-3539665 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-35396652013-01-14 Secreted Factors from Bone Marrow Stromal Cells Upregulate IL-10 and Reverse Acute Kidney Injury Milwid, Jack M. Ichimura, Takaharu Li, Matthew Jiao, Yunxin Lee, Jungwoo Yarmush, Joshua S. Parekkadan, Biju Tilles, Arno W. Bonventre, Joseph V. Yarmush, Martin L. Stem Cells Int Research Article Acute kidney injury is a devastating syndrome that afflicts over 2,000,000 people in the US per year, with an associated mortality of greater than 70% in severe cases. Unfortunately, standard-of-care treatments are not sufficient for modifying the course of disease. Many groups have explored the use of bone marrow stromal cells (BMSCs) for the treatment of AKI because BMSCs have been shown to possess unique anti-inflammatory, cytoprotective, and regenerative properties in vitro and in vivo. It is yet unresolved whether the primary mechanisms controlling BMSC therapy in AKI depend on direct cell infusion, or whether BMSC-secreted factors alone are sufficient for mitigating the injury. Here we show that BMSC-secreted factors are capable of providing a survival benefit to rats subjected to cisplatin-induced AKI. We observed that when BMSC-conditioned medium (BMSC-CM) is administered intravenously, it prevents tubular apoptosis and necrosis and ameliorates AKI. In addition, we observed that BMSC-CM causes IL-10 upregulation in treated animals, which is important to animal survival and protection of the kidney. In all, these results demonstrate that BMSC-secreted factors are capable of providing support without cell transplantation, and the IL-10 increase seen in BMSC-CM-treated animals correlates with attenuation of severe AKI. Hindawi Publishing Corporation 2012 2012-12-19 /pmc/articles/PMC3539665/ /pubmed/23319959 http://dx.doi.org/10.1155/2012/392050 Text en Copyright © 2012 Jack M. Milwid et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Milwid, Jack M. Ichimura, Takaharu Li, Matthew Jiao, Yunxin Lee, Jungwoo Yarmush, Joshua S. Parekkadan, Biju Tilles, Arno W. Bonventre, Joseph V. Yarmush, Martin L. Secreted Factors from Bone Marrow Stromal Cells Upregulate IL-10 and Reverse Acute Kidney Injury |
title | Secreted Factors from Bone Marrow Stromal Cells Upregulate IL-10 and Reverse Acute Kidney Injury |
title_full | Secreted Factors from Bone Marrow Stromal Cells Upregulate IL-10 and Reverse Acute Kidney Injury |
title_fullStr | Secreted Factors from Bone Marrow Stromal Cells Upregulate IL-10 and Reverse Acute Kidney Injury |
title_full_unstemmed | Secreted Factors from Bone Marrow Stromal Cells Upregulate IL-10 and Reverse Acute Kidney Injury |
title_short | Secreted Factors from Bone Marrow Stromal Cells Upregulate IL-10 and Reverse Acute Kidney Injury |
title_sort | secreted factors from bone marrow stromal cells upregulate il-10 and reverse acute kidney injury |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539665/ https://www.ncbi.nlm.nih.gov/pubmed/23319959 http://dx.doi.org/10.1155/2012/392050 |
work_keys_str_mv | AT milwidjackm secretedfactorsfrombonemarrowstromalcellsupregulateil10andreverseacutekidneyinjury AT ichimuratakaharu secretedfactorsfrombonemarrowstromalcellsupregulateil10andreverseacutekidneyinjury AT limatthew secretedfactorsfrombonemarrowstromalcellsupregulateil10andreverseacutekidneyinjury AT jiaoyunxin secretedfactorsfrombonemarrowstromalcellsupregulateil10andreverseacutekidneyinjury AT leejungwoo secretedfactorsfrombonemarrowstromalcellsupregulateil10andreverseacutekidneyinjury AT yarmushjoshuas secretedfactorsfrombonemarrowstromalcellsupregulateil10andreverseacutekidneyinjury AT parekkadanbiju secretedfactorsfrombonemarrowstromalcellsupregulateil10andreverseacutekidneyinjury AT tillesarnow secretedfactorsfrombonemarrowstromalcellsupregulateil10andreverseacutekidneyinjury AT bonventrejosephv secretedfactorsfrombonemarrowstromalcellsupregulateil10andreverseacutekidneyinjury AT yarmushmartinl secretedfactorsfrombonemarrowstromalcellsupregulateil10andreverseacutekidneyinjury |