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A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments

BACKGROUND: Molecular cloning of DNA fragments >5 kbp is still a complex task. When no genomic DNA library is available for the species of interest, and direct PCR amplification of the desired DNA fragment is unsuccessful or results in an incorrect sequence, molecular cloning of a PCR-amplified r...

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Detalles Bibliográficos
Autores principales:	Orlova, Nadezhda A, Orlov, Alexandre V, Vorobiev, Ivan I
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Technical Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539906/ https://www.ncbi.nlm.nih.gov/pubmed/22709633 http://dx.doi.org/10.1186/1756-0500-5-303

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