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Staphylococcus aureus autoinducer-2 quorum sensing decreases biofilm formation in an icaR-dependent manner

BACKGROUND: Staphylococcus aureus is an important pathogen that causes biofilm-associated infection in humans. Autoinducer 2 (AI-2), a quorum-sensing (QS) signal for interspecies communication, has a wide range of regulatory functions in both Gram-positive and Gram-negative bacteria, but its exact r...

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Autores principales: Yu, Dan, Zhao, Liping, Xue, Ting, Sun, Baolin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539994/
https://www.ncbi.nlm.nih.gov/pubmed/23216979
http://dx.doi.org/10.1186/1471-2180-12-288
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author Yu, Dan
Zhao, Liping
Xue, Ting
Sun, Baolin
author_facet Yu, Dan
Zhao, Liping
Xue, Ting
Sun, Baolin
author_sort Yu, Dan
collection PubMed
description BACKGROUND: Staphylococcus aureus is an important pathogen that causes biofilm-associated infection in humans. Autoinducer 2 (AI-2), a quorum-sensing (QS) signal for interspecies communication, has a wide range of regulatory functions in both Gram-positive and Gram-negative bacteria, but its exact role in biofilm formation in S. aureus remains unclear. RESULTS: Here we demonstrate that mutation of the AI-2 synthase gene luxS in S. aureus RN6390B results in increased biofilm formation compared with the wild-type (WT) strain under static, flowing and anaerobic conditions and in a mouse model. Addition of the chemically synthesized AI-2 precursor in the luxS mutation strain (ΔluxS) restored the WT phenotype. Real-time RT-PCR analysis showed that AI-2 activated the transcription of icaR, a repressor of the ica operon, and subsequently a decreased level of icaA transcription, which was presumably the main reason why luxS mutation influences biofilm formation. Furthermore, we compared the roles of the agr-mediated QS system and the LuxS/AI-2 QS system in the regulation of biofilm formation using the ΔluxS strain, RN6911 and the Δagr ΔluxS strain. Our data indicate a cumulative effect of the two QS systems on the regulation of biofilm formation in S. aureus. CONCLUSION: These findings demonstrate that AI-2 can decrease biofilm formation in S. aureus via an icaR-activation pathway. This study may provide clues for therapy in S. aureus biofilm-associated infection.
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spelling pubmed-35399942013-01-10 Staphylococcus aureus autoinducer-2 quorum sensing decreases biofilm formation in an icaR-dependent manner Yu, Dan Zhao, Liping Xue, Ting Sun, Baolin BMC Microbiol Research Article BACKGROUND: Staphylococcus aureus is an important pathogen that causes biofilm-associated infection in humans. Autoinducer 2 (AI-2), a quorum-sensing (QS) signal for interspecies communication, has a wide range of regulatory functions in both Gram-positive and Gram-negative bacteria, but its exact role in biofilm formation in S. aureus remains unclear. RESULTS: Here we demonstrate that mutation of the AI-2 synthase gene luxS in S. aureus RN6390B results in increased biofilm formation compared with the wild-type (WT) strain under static, flowing and anaerobic conditions and in a mouse model. Addition of the chemically synthesized AI-2 precursor in the luxS mutation strain (ΔluxS) restored the WT phenotype. Real-time RT-PCR analysis showed that AI-2 activated the transcription of icaR, a repressor of the ica operon, and subsequently a decreased level of icaA transcription, which was presumably the main reason why luxS mutation influences biofilm formation. Furthermore, we compared the roles of the agr-mediated QS system and the LuxS/AI-2 QS system in the regulation of biofilm formation using the ΔluxS strain, RN6911 and the Δagr ΔluxS strain. Our data indicate a cumulative effect of the two QS systems on the regulation of biofilm formation in S. aureus. CONCLUSION: These findings demonstrate that AI-2 can decrease biofilm formation in S. aureus via an icaR-activation pathway. This study may provide clues for therapy in S. aureus biofilm-associated infection. BioMed Central 2012-12-05 /pmc/articles/PMC3539994/ /pubmed/23216979 http://dx.doi.org/10.1186/1471-2180-12-288 Text en Copyright ©2012 Yu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yu, Dan
Zhao, Liping
Xue, Ting
Sun, Baolin
Staphylococcus aureus autoinducer-2 quorum sensing decreases biofilm formation in an icaR-dependent manner
title Staphylococcus aureus autoinducer-2 quorum sensing decreases biofilm formation in an icaR-dependent manner
title_full Staphylococcus aureus autoinducer-2 quorum sensing decreases biofilm formation in an icaR-dependent manner
title_fullStr Staphylococcus aureus autoinducer-2 quorum sensing decreases biofilm formation in an icaR-dependent manner
title_full_unstemmed Staphylococcus aureus autoinducer-2 quorum sensing decreases biofilm formation in an icaR-dependent manner
title_short Staphylococcus aureus autoinducer-2 quorum sensing decreases biofilm formation in an icaR-dependent manner
title_sort staphylococcus aureus autoinducer-2 quorum sensing decreases biofilm formation in an icar-dependent manner
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539994/
https://www.ncbi.nlm.nih.gov/pubmed/23216979
http://dx.doi.org/10.1186/1471-2180-12-288
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