Cargando…

Assessing Breast Cancer Margins Ex Vivo Using Aqueous Quantum-Dot-Molecular Probes

Positive margins have been a critical issue that hinders the success of breast- conserving surgery. The incidence of positive margins is estimated to range from 20% to as high as 60%. Currently, there is no effective intraoperative method for margin assessment. It would be desirable if there is a ra...

Descripción completa

Detalles Bibliográficos
Autores principales: Au, Giang H. T., Shih, Wan Y., Shih, Wei-Heng, Mejias, Linette, Swami, Vanlila K., Wasko, Kimberly, Brooks, Ari D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3540809/
https://www.ncbi.nlm.nih.gov/pubmed/23320158
http://dx.doi.org/10.1155/2012/861257
_version_ 1782255261729161216
author Au, Giang H. T.
Shih, Wan Y.
Shih, Wei-Heng
Mejias, Linette
Swami, Vanlila K.
Wasko, Kimberly
Brooks, Ari D.
author_facet Au, Giang H. T.
Shih, Wan Y.
Shih, Wei-Heng
Mejias, Linette
Swami, Vanlila K.
Wasko, Kimberly
Brooks, Ari D.
author_sort Au, Giang H. T.
collection PubMed
description Positive margins have been a critical issue that hinders the success of breast- conserving surgery. The incidence of positive margins is estimated to range from 20% to as high as 60%. Currently, there is no effective intraoperative method for margin assessment. It would be desirable if there is a rapid and reliable breast cancer margin assessment tool in the operating room so that further surgery can be continued if necessary to reduce re-excision rate. In this study, we seek to develop a sensitive and specific molecular probe to help surgeons assess if the surgical margin is clean. The molecular probe consists of the unique aqueous quantum dots developed in our laboratory conjugated with antibodies specific to breast cancer markers such as Tn-antigen. Excised tumors from tumor-bearing nude mice were used to demonstrate the method. AQD-Tn mAb probe proved to be sensitive and specific to identify cancer area quantitatively without being affected by the heterogeneity of the tissue. The integrity of the surgical specimen was not affected by the AQD treatment. Furthermore, AQD-Tn mAb method could determine margin status within 30 minutes of tumor excision, indicating its potential as an accurate intraoperative margin assessment method.
format Online
Article
Text
id pubmed-3540809
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-35408092013-01-14 Assessing Breast Cancer Margins Ex Vivo Using Aqueous Quantum-Dot-Molecular Probes Au, Giang H. T. Shih, Wan Y. Shih, Wei-Heng Mejias, Linette Swami, Vanlila K. Wasko, Kimberly Brooks, Ari D. Int J Surg Oncol Research Article Positive margins have been a critical issue that hinders the success of breast- conserving surgery. The incidence of positive margins is estimated to range from 20% to as high as 60%. Currently, there is no effective intraoperative method for margin assessment. It would be desirable if there is a rapid and reliable breast cancer margin assessment tool in the operating room so that further surgery can be continued if necessary to reduce re-excision rate. In this study, we seek to develop a sensitive and specific molecular probe to help surgeons assess if the surgical margin is clean. The molecular probe consists of the unique aqueous quantum dots developed in our laboratory conjugated with antibodies specific to breast cancer markers such as Tn-antigen. Excised tumors from tumor-bearing nude mice were used to demonstrate the method. AQD-Tn mAb probe proved to be sensitive and specific to identify cancer area quantitatively without being affected by the heterogeneity of the tissue. The integrity of the surgical specimen was not affected by the AQD treatment. Furthermore, AQD-Tn mAb method could determine margin status within 30 minutes of tumor excision, indicating its potential as an accurate intraoperative margin assessment method. Hindawi Publishing Corporation 2012 2012-12-24 /pmc/articles/PMC3540809/ /pubmed/23320158 http://dx.doi.org/10.1155/2012/861257 Text en Copyright © 2012 Giang H. T. Au et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Au, Giang H. T.
Shih, Wan Y.
Shih, Wei-Heng
Mejias, Linette
Swami, Vanlila K.
Wasko, Kimberly
Brooks, Ari D.
Assessing Breast Cancer Margins Ex Vivo Using Aqueous Quantum-Dot-Molecular Probes
title Assessing Breast Cancer Margins Ex Vivo Using Aqueous Quantum-Dot-Molecular Probes
title_full Assessing Breast Cancer Margins Ex Vivo Using Aqueous Quantum-Dot-Molecular Probes
title_fullStr Assessing Breast Cancer Margins Ex Vivo Using Aqueous Quantum-Dot-Molecular Probes
title_full_unstemmed Assessing Breast Cancer Margins Ex Vivo Using Aqueous Quantum-Dot-Molecular Probes
title_short Assessing Breast Cancer Margins Ex Vivo Using Aqueous Quantum-Dot-Molecular Probes
title_sort assessing breast cancer margins ex vivo using aqueous quantum-dot-molecular probes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3540809/
https://www.ncbi.nlm.nih.gov/pubmed/23320158
http://dx.doi.org/10.1155/2012/861257
work_keys_str_mv AT augianght assessingbreastcancermarginsexvivousingaqueousquantumdotmolecularprobes
AT shihwany assessingbreastcancermarginsexvivousingaqueousquantumdotmolecularprobes
AT shihweiheng assessingbreastcancermarginsexvivousingaqueousquantumdotmolecularprobes
AT mejiaslinette assessingbreastcancermarginsexvivousingaqueousquantumdotmolecularprobes
AT swamivanlilak assessingbreastcancermarginsexvivousingaqueousquantumdotmolecularprobes
AT waskokimberly assessingbreastcancermarginsexvivousingaqueousquantumdotmolecularprobes
AT brooksarid assessingbreastcancermarginsexvivousingaqueousquantumdotmolecularprobes