Comment on Kokshoorn, B, and Blankers, BJ ‘Response to Grisedale, KS and van Daal, A: comparison of STR profiling from low template DNA extracts with and without the consensus profiling method’

Kokshoorn and Blankers responded to our recent article by saying that replicate analysis and consensus profiling of low template samples was best in terms of reliability and objectivity. We agree that the consensus approach has benefits, particularly in eliminating non-repeating spurious alleles fro...

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Autores principales: Grisedale, Kelly, van Daal, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541072/
https://www.ncbi.nlm.nih.gov/pubmed/23286588
http://dx.doi.org/10.1186/2041-2223-4-2
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author Grisedale, Kelly
van Daal, Angela
author_facet Grisedale, Kelly
van Daal, Angela
author_sort Grisedale, Kelly
collection PubMed
description Kokshoorn and Blankers responded to our recent article by saying that replicate analysis and consensus profiling of low template samples was best in terms of reliability and objectivity. We agree that the consensus approach has benefits, particularly in eliminating non-repeating spurious alleles from the final profile. However, with the development of statistical models that can accommodate stochastic effects and allele drop in, it may be beneficial to perform a single amplification with three times the amount of template, since much information is lost from the profile using the consensus approach.
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spelling pubmed-35410722013-01-11 Comment on Kokshoorn, B, and Blankers, BJ ‘Response to Grisedale, KS and van Daal, A: comparison of STR profiling from low template DNA extracts with and without the consensus profiling method’ Grisedale, Kelly van Daal, Angela Investig Genet Letter to the Editor Kokshoorn and Blankers responded to our recent article by saying that replicate analysis and consensus profiling of low template samples was best in terms of reliability and objectivity. We agree that the consensus approach has benefits, particularly in eliminating non-repeating spurious alleles from the final profile. However, with the development of statistical models that can accommodate stochastic effects and allele drop in, it may be beneficial to perform a single amplification with three times the amount of template, since much information is lost from the profile using the consensus approach. BioMed Central 2013-01-03 /pmc/articles/PMC3541072/ /pubmed/23286588 http://dx.doi.org/10.1186/2041-2223-4-2 Text en Copyright ©2013 Grisedale and Daal; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Letter to the Editor
Grisedale, Kelly
van Daal, Angela
Comment on Kokshoorn, B, and Blankers, BJ ‘Response to Grisedale, KS and van Daal, A: comparison of STR profiling from low template DNA extracts with and without the consensus profiling method’
title Comment on Kokshoorn, B, and Blankers, BJ ‘Response to Grisedale, KS and van Daal, A: comparison of STR profiling from low template DNA extracts with and without the consensus profiling method’
title_full Comment on Kokshoorn, B, and Blankers, BJ ‘Response to Grisedale, KS and van Daal, A: comparison of STR profiling from low template DNA extracts with and without the consensus profiling method’
title_fullStr Comment on Kokshoorn, B, and Blankers, BJ ‘Response to Grisedale, KS and van Daal, A: comparison of STR profiling from low template DNA extracts with and without the consensus profiling method’
title_full_unstemmed Comment on Kokshoorn, B, and Blankers, BJ ‘Response to Grisedale, KS and van Daal, A: comparison of STR profiling from low template DNA extracts with and without the consensus profiling method’
title_short Comment on Kokshoorn, B, and Blankers, BJ ‘Response to Grisedale, KS and van Daal, A: comparison of STR profiling from low template DNA extracts with and without the consensus profiling method’
title_sort comment on kokshoorn, b, and blankers, bj ‘response to grisedale, ks and van daal, a: comparison of str profiling from low template dna extracts with and without the consensus profiling method’
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541072/
https://www.ncbi.nlm.nih.gov/pubmed/23286588
http://dx.doi.org/10.1186/2041-2223-4-2
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