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Genome characterization and population genetic structure of the zoonotic pathogen, Streptococcus canis

BACKGROUND: Streptococcus canis is an important opportunistic pathogen of dogs and cats that can also infect a wide range of additional mammals including cows where it can cause mastitis. It is also an emerging human pathogen. RESULTS: Here we provide characterization of the first genome sequence fo...

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Autores principales: Richards, Vincent P, Zadoks, Ruth N, Pavinski Bitar, Paulina D, Lefébure, Tristan, Lang, Ping, Werner, Brenda, Tikofsky, Linda, Moroni, Paolo, Stanhope, Michael J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541175/
https://www.ncbi.nlm.nih.gov/pubmed/23244770
http://dx.doi.org/10.1186/1471-2180-12-293
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author Richards, Vincent P
Zadoks, Ruth N
Pavinski Bitar, Paulina D
Lefébure, Tristan
Lang, Ping
Werner, Brenda
Tikofsky, Linda
Moroni, Paolo
Stanhope, Michael J
author_facet Richards, Vincent P
Zadoks, Ruth N
Pavinski Bitar, Paulina D
Lefébure, Tristan
Lang, Ping
Werner, Brenda
Tikofsky, Linda
Moroni, Paolo
Stanhope, Michael J
author_sort Richards, Vincent P
collection PubMed
description BACKGROUND: Streptococcus canis is an important opportunistic pathogen of dogs and cats that can also infect a wide range of additional mammals including cows where it can cause mastitis. It is also an emerging human pathogen. RESULTS: Here we provide characterization of the first genome sequence for this species, strain FSL S3-227 (milk isolate from a cow with an intra-mammary infection). A diverse array of putative virulence factors was encoded by the S. canis FSL S3-227 genome. Approximately 75% of these gene sequences were homologous to known Streptococcal virulence factors involved in invasion, evasion, and colonization. Present in the genome are multiple potentially mobile genetic elements (MGEs) [plasmid, phage, integrative conjugative element (ICE)] and comparison to other species provided convincing evidence for lateral gene transfer (LGT) between S. canis and two additional bovine mastitis causing pathogens (Streptococcus agalactiae, and Streptococcus dysgalactiae subsp. dysgalactiae), with this transfer possibly contributing to host adaptation. Population structure among isolates obtained from Europe and USA [bovine = 56, canine = 26, and feline = 1] was explored. Ribotyping of all isolates and multi locus sequence typing (MLST) of a subset of the isolates (n = 45) detected significant differentiation between bovine and canine isolates (Fisher exact test: P = 0.0000 [ribotypes], P = 0.0030 [sequence types]), suggesting possible host adaptation of some genotypes. Concurrently, the ancestral clonal complex (54% of isolates) occurred in many tissue types, all hosts, and all geographic locations suggesting the possibility of a wide and diverse niche. CONCLUSION: This study provides evidence highlighting the importance of LGT in the evolution of the bacteria S. canis, specifically, its possible role in host adaptation and acquisition of virulence factors. Furthermore, recent LGT detected between S. canis and human bacteria (Streptococcus urinalis) is cause for concern, as it highlights the possibility for continued acquisition of human virulence factors for this emerging zoonotic pathogen.
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spelling pubmed-35411752013-01-11 Genome characterization and population genetic structure of the zoonotic pathogen, Streptococcus canis Richards, Vincent P Zadoks, Ruth N Pavinski Bitar, Paulina D Lefébure, Tristan Lang, Ping Werner, Brenda Tikofsky, Linda Moroni, Paolo Stanhope, Michael J BMC Microbiol Research Article BACKGROUND: Streptococcus canis is an important opportunistic pathogen of dogs and cats that can also infect a wide range of additional mammals including cows where it can cause mastitis. It is also an emerging human pathogen. RESULTS: Here we provide characterization of the first genome sequence for this species, strain FSL S3-227 (milk isolate from a cow with an intra-mammary infection). A diverse array of putative virulence factors was encoded by the S. canis FSL S3-227 genome. Approximately 75% of these gene sequences were homologous to known Streptococcal virulence factors involved in invasion, evasion, and colonization. Present in the genome are multiple potentially mobile genetic elements (MGEs) [plasmid, phage, integrative conjugative element (ICE)] and comparison to other species provided convincing evidence for lateral gene transfer (LGT) between S. canis and two additional bovine mastitis causing pathogens (Streptococcus agalactiae, and Streptococcus dysgalactiae subsp. dysgalactiae), with this transfer possibly contributing to host adaptation. Population structure among isolates obtained from Europe and USA [bovine = 56, canine = 26, and feline = 1] was explored. Ribotyping of all isolates and multi locus sequence typing (MLST) of a subset of the isolates (n = 45) detected significant differentiation between bovine and canine isolates (Fisher exact test: P = 0.0000 [ribotypes], P = 0.0030 [sequence types]), suggesting possible host adaptation of some genotypes. Concurrently, the ancestral clonal complex (54% of isolates) occurred in many tissue types, all hosts, and all geographic locations suggesting the possibility of a wide and diverse niche. CONCLUSION: This study provides evidence highlighting the importance of LGT in the evolution of the bacteria S. canis, specifically, its possible role in host adaptation and acquisition of virulence factors. Furthermore, recent LGT detected between S. canis and human bacteria (Streptococcus urinalis) is cause for concern, as it highlights the possibility for continued acquisition of human virulence factors for this emerging zoonotic pathogen. BioMed Central 2012-12-18 /pmc/articles/PMC3541175/ /pubmed/23244770 http://dx.doi.org/10.1186/1471-2180-12-293 Text en Copyright ©2012 Richards et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Richards, Vincent P
Zadoks, Ruth N
Pavinski Bitar, Paulina D
Lefébure, Tristan
Lang, Ping
Werner, Brenda
Tikofsky, Linda
Moroni, Paolo
Stanhope, Michael J
Genome characterization and population genetic structure of the zoonotic pathogen, Streptococcus canis
title Genome characterization and population genetic structure of the zoonotic pathogen, Streptococcus canis
title_full Genome characterization and population genetic structure of the zoonotic pathogen, Streptococcus canis
title_fullStr Genome characterization and population genetic structure of the zoonotic pathogen, Streptococcus canis
title_full_unstemmed Genome characterization and population genetic structure of the zoonotic pathogen, Streptococcus canis
title_short Genome characterization and population genetic structure of the zoonotic pathogen, Streptococcus canis
title_sort genome characterization and population genetic structure of the zoonotic pathogen, streptococcus canis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541175/
https://www.ncbi.nlm.nih.gov/pubmed/23244770
http://dx.doi.org/10.1186/1471-2180-12-293
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