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Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species

BACKGROUND: Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most importan...

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Autores principales: Stone, Joshua K, Mayo, Mark, Grasso, Stephanie A, Ginther, Jennifer L, Warrington, Stephanie D, Allender, Christopher J, Doyle, Adina, Georgia, Shalamar, Kaestli, Mirjam, Broomall, Stacey M, Karavis, Mark A, Insalaco, Joseph M, Hubbard, Kyle S, McNew, Lauren A, Gibbons, Henry S, Currie, Bart J, Keim, Paul, Tuanyok, Apichai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541218/
https://www.ncbi.nlm.nih.gov/pubmed/23126230
http://dx.doi.org/10.1186/1471-2180-12-250
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author Stone, Joshua K
Mayo, Mark
Grasso, Stephanie A
Ginther, Jennifer L
Warrington, Stephanie D
Allender, Christopher J
Doyle, Adina
Georgia, Shalamar
Kaestli, Mirjam
Broomall, Stacey M
Karavis, Mark A
Insalaco, Joseph M
Hubbard, Kyle S
McNew, Lauren A
Gibbons, Henry S
Currie, Bart J
Keim, Paul
Tuanyok, Apichai
author_facet Stone, Joshua K
Mayo, Mark
Grasso, Stephanie A
Ginther, Jennifer L
Warrington, Stephanie D
Allender, Christopher J
Doyle, Adina
Georgia, Shalamar
Kaestli, Mirjam
Broomall, Stacey M
Karavis, Mark A
Insalaco, Joseph M
Hubbard, Kyle S
McNew, Lauren A
Gibbons, Henry S
Currie, Bart J
Keim, Paul
Tuanyok, Apichai
author_sort Stone, Joshua K
collection PubMed
description BACKGROUND: Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most important antigenic epitopes in B. pseudomallei. Complicating this strategy are the four different B. pseudomallei LPS O-antigen types: A, B, B2, and rough. Sero-crossreactivity is common among O-antigens of Burkholderia species. Here, we identified the presence of multiple B. pseudomallei O-antigen types and sero-crossreactivity in its near-neighbor species. RESULTS: PCR screening of O-antigen biosynthesis genes, phenotypic characterization using SDS-PAGE, and immunoblot analysis showed that majority of B. mallei and B. thailandensis strains contained the typical O-antigen type A. In contrast, most of B. ubonensis and B. thailandensis-like strains expressed the atypical O-antigen types B and B2, respectively. Most B. oklahomensis strains expressed a distinct and non-seroreactive O-antigen type, except strain E0147 which expressed O-antigen type A. O-antigen type B2 was also detected in B. thailandensis 82172, B. ubonensis MSMB108, and Burkholderia sp. MSMB175. Interestingly, B. thailandensis-like MSMB43 contained a novel serotype B positive O-antigen. CONCLUSIONS: This study expands the number of species which express B. pseudomallei O-antigen types. Further work is required to elucidate the full structures and how closely these are to the B. pseudomallei O-antigens, which will ultimately determine the efficacy of the near-neighbor B serotypes for vaccine development.
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spelling pubmed-35412182013-01-11 Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species Stone, Joshua K Mayo, Mark Grasso, Stephanie A Ginther, Jennifer L Warrington, Stephanie D Allender, Christopher J Doyle, Adina Georgia, Shalamar Kaestli, Mirjam Broomall, Stacey M Karavis, Mark A Insalaco, Joseph M Hubbard, Kyle S McNew, Lauren A Gibbons, Henry S Currie, Bart J Keim, Paul Tuanyok, Apichai BMC Microbiol Research Article BACKGROUND: Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most important antigenic epitopes in B. pseudomallei. Complicating this strategy are the four different B. pseudomallei LPS O-antigen types: A, B, B2, and rough. Sero-crossreactivity is common among O-antigens of Burkholderia species. Here, we identified the presence of multiple B. pseudomallei O-antigen types and sero-crossreactivity in its near-neighbor species. RESULTS: PCR screening of O-antigen biosynthesis genes, phenotypic characterization using SDS-PAGE, and immunoblot analysis showed that majority of B. mallei and B. thailandensis strains contained the typical O-antigen type A. In contrast, most of B. ubonensis and B. thailandensis-like strains expressed the atypical O-antigen types B and B2, respectively. Most B. oklahomensis strains expressed a distinct and non-seroreactive O-antigen type, except strain E0147 which expressed O-antigen type A. O-antigen type B2 was also detected in B. thailandensis 82172, B. ubonensis MSMB108, and Burkholderia sp. MSMB175. Interestingly, B. thailandensis-like MSMB43 contained a novel serotype B positive O-antigen. CONCLUSIONS: This study expands the number of species which express B. pseudomallei O-antigen types. Further work is required to elucidate the full structures and how closely these are to the B. pseudomallei O-antigens, which will ultimately determine the efficacy of the near-neighbor B serotypes for vaccine development. BioMed Central 2012-11-05 /pmc/articles/PMC3541218/ /pubmed/23126230 http://dx.doi.org/10.1186/1471-2180-12-250 Text en Copyright ©2012 Stone et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Stone, Joshua K
Mayo, Mark
Grasso, Stephanie A
Ginther, Jennifer L
Warrington, Stephanie D
Allender, Christopher J
Doyle, Adina
Georgia, Shalamar
Kaestli, Mirjam
Broomall, Stacey M
Karavis, Mark A
Insalaco, Joseph M
Hubbard, Kyle S
McNew, Lauren A
Gibbons, Henry S
Currie, Bart J
Keim, Paul
Tuanyok, Apichai
Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species
title Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species
title_full Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species
title_fullStr Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species
title_full_unstemmed Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species
title_short Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species
title_sort detection of burkholderia pseudomallei o-antigen serotypes in near-neighbor species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541218/
https://www.ncbi.nlm.nih.gov/pubmed/23126230
http://dx.doi.org/10.1186/1471-2180-12-250
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