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Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells
We investigated the effects of anticancer agents on peripheral blood mononuclear cells for the purpose of providing data to support new translational chemoimmunotherapy regimens. Peripheral-blood mononuclear cells were treated with one of four anticancer agents (5-fluorouracil, irinotecan, cisplatin...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
the Society for Free Radical Research Japan
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541421/ https://www.ncbi.nlm.nih.gov/pubmed/23341700 http://dx.doi.org/10.3164/jcbn.12-60 |
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author | Sakai, Hiromi Kokura, Satoshi Ishikawa, Takeshi Tsuchiya, Reiko Okajima, Manabu Matsuyama, Tatsuzou Adachi, Satoko Katada, Kazuhiro Kamada, Kazuhiro Uchiyama, Kazuhiko Handa, Osamu Takagi, Tomohisa Yagi, Nobuaki Naito, Yuji Yoshikawa, Toshikazu |
author_facet | Sakai, Hiromi Kokura, Satoshi Ishikawa, Takeshi Tsuchiya, Reiko Okajima, Manabu Matsuyama, Tatsuzou Adachi, Satoko Katada, Kazuhiro Kamada, Kazuhiro Uchiyama, Kazuhiko Handa, Osamu Takagi, Tomohisa Yagi, Nobuaki Naito, Yuji Yoshikawa, Toshikazu |
author_sort | Sakai, Hiromi |
collection | PubMed |
description | We investigated the effects of anticancer agents on peripheral blood mononuclear cells for the purpose of providing data to support new translational chemoimmunotherapy regimens. Peripheral-blood mononuclear cells were treated with one of four anticancer agents (5-fluorouracil, irinotecan, cisplatin, and gemcitabine) for 2 h, after which cell viability was determined. For assessment of effects of each drug on proliferation and cytokine production, cells were stimulated with phytohemagglutinin for 48 h. As a result, the anticancer agents did not affect cell viability. Cell proliferation was unaffected by 5-fluorouracil and irinotecan but inhibited by cisplatin and gemcitabine. Treatment with gemcitabine enhanced the production of IFN-γ and decreased the number of regulatory T cells. gemcitabine treatment increased IFN-γ production among CD4 T cells but not among CD8 T cells. The results indicated that GEM had immunoregulatory properties that might support immune response against cancer. This finding has implications for designing chemoimmunotherapy strategies. |
format | Online Article Text |
id | pubmed-3541421 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | the Society for Free Radical Research Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-35414212013-01-22 Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells Sakai, Hiromi Kokura, Satoshi Ishikawa, Takeshi Tsuchiya, Reiko Okajima, Manabu Matsuyama, Tatsuzou Adachi, Satoko Katada, Kazuhiro Kamada, Kazuhiro Uchiyama, Kazuhiko Handa, Osamu Takagi, Tomohisa Yagi, Nobuaki Naito, Yuji Yoshikawa, Toshikazu J Clin Biochem Nutr Original Article We investigated the effects of anticancer agents on peripheral blood mononuclear cells for the purpose of providing data to support new translational chemoimmunotherapy regimens. Peripheral-blood mononuclear cells were treated with one of four anticancer agents (5-fluorouracil, irinotecan, cisplatin, and gemcitabine) for 2 h, after which cell viability was determined. For assessment of effects of each drug on proliferation and cytokine production, cells were stimulated with phytohemagglutinin for 48 h. As a result, the anticancer agents did not affect cell viability. Cell proliferation was unaffected by 5-fluorouracil and irinotecan but inhibited by cisplatin and gemcitabine. Treatment with gemcitabine enhanced the production of IFN-γ and decreased the number of regulatory T cells. gemcitabine treatment increased IFN-γ production among CD4 T cells but not among CD8 T cells. The results indicated that GEM had immunoregulatory properties that might support immune response against cancer. This finding has implications for designing chemoimmunotherapy strategies. the Society for Free Radical Research Japan 2013-01 2012-11-20 /pmc/articles/PMC3541421/ /pubmed/23341700 http://dx.doi.org/10.3164/jcbn.12-60 Text en Copyright © 2013 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Sakai, Hiromi Kokura, Satoshi Ishikawa, Takeshi Tsuchiya, Reiko Okajima, Manabu Matsuyama, Tatsuzou Adachi, Satoko Katada, Kazuhiro Kamada, Kazuhiro Uchiyama, Kazuhiko Handa, Osamu Takagi, Tomohisa Yagi, Nobuaki Naito, Yuji Yoshikawa, Toshikazu Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells |
title | Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells |
title_full | Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells |
title_fullStr | Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells |
title_full_unstemmed | Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells |
title_short | Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells |
title_sort | effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541421/ https://www.ncbi.nlm.nih.gov/pubmed/23341700 http://dx.doi.org/10.3164/jcbn.12-60 |
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