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Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells

We investigated the effects of anticancer agents on peripheral blood mononuclear cells for the purpose of providing data to support new translational chemoimmunotherapy regimens. Peripheral-blood mononuclear cells were treated with one of four anticancer agents (5-fluorouracil, irinotecan, cisplatin...

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Autores principales: Sakai, Hiromi, Kokura, Satoshi, Ishikawa, Takeshi, Tsuchiya, Reiko, Okajima, Manabu, Matsuyama, Tatsuzou, Adachi, Satoko, Katada, Kazuhiro, Kamada, Kazuhiro, Uchiyama, Kazuhiko, Handa, Osamu, Takagi, Tomohisa, Yagi, Nobuaki, Naito, Yuji, Yoshikawa, Toshikazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541421/
https://www.ncbi.nlm.nih.gov/pubmed/23341700
http://dx.doi.org/10.3164/jcbn.12-60
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author Sakai, Hiromi
Kokura, Satoshi
Ishikawa, Takeshi
Tsuchiya, Reiko
Okajima, Manabu
Matsuyama, Tatsuzou
Adachi, Satoko
Katada, Kazuhiro
Kamada, Kazuhiro
Uchiyama, Kazuhiko
Handa, Osamu
Takagi, Tomohisa
Yagi, Nobuaki
Naito, Yuji
Yoshikawa, Toshikazu
author_facet Sakai, Hiromi
Kokura, Satoshi
Ishikawa, Takeshi
Tsuchiya, Reiko
Okajima, Manabu
Matsuyama, Tatsuzou
Adachi, Satoko
Katada, Kazuhiro
Kamada, Kazuhiro
Uchiyama, Kazuhiko
Handa, Osamu
Takagi, Tomohisa
Yagi, Nobuaki
Naito, Yuji
Yoshikawa, Toshikazu
author_sort Sakai, Hiromi
collection PubMed
description We investigated the effects of anticancer agents on peripheral blood mononuclear cells for the purpose of providing data to support new translational chemoimmunotherapy regimens. Peripheral-blood mononuclear cells were treated with one of four anticancer agents (5-fluorouracil, irinotecan, cisplatin, and gemcitabine) for 2 h, after which cell viability was determined. For assessment of effects of each drug on proliferation and cytokine production, cells were stimulated with phytohemagglutinin for 48 h. As a result, the anticancer agents did not affect cell viability. Cell proliferation was unaffected by 5-fluorouracil and irinotecan but inhibited by cisplatin and gemcitabine. Treatment with gemcitabine enhanced the production of IFN-γ and decreased the number of regulatory T cells. gemcitabine treatment increased IFN-γ production among CD4 T cells but not among CD8 T cells. The results indicated that GEM had immunoregulatory properties that might support immune response against cancer. This finding has implications for designing chemoimmunotherapy strategies.
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spelling pubmed-35414212013-01-22 Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells Sakai, Hiromi Kokura, Satoshi Ishikawa, Takeshi Tsuchiya, Reiko Okajima, Manabu Matsuyama, Tatsuzou Adachi, Satoko Katada, Kazuhiro Kamada, Kazuhiro Uchiyama, Kazuhiko Handa, Osamu Takagi, Tomohisa Yagi, Nobuaki Naito, Yuji Yoshikawa, Toshikazu J Clin Biochem Nutr Original Article We investigated the effects of anticancer agents on peripheral blood mononuclear cells for the purpose of providing data to support new translational chemoimmunotherapy regimens. Peripheral-blood mononuclear cells were treated with one of four anticancer agents (5-fluorouracil, irinotecan, cisplatin, and gemcitabine) for 2 h, after which cell viability was determined. For assessment of effects of each drug on proliferation and cytokine production, cells were stimulated with phytohemagglutinin for 48 h. As a result, the anticancer agents did not affect cell viability. Cell proliferation was unaffected by 5-fluorouracil and irinotecan but inhibited by cisplatin and gemcitabine. Treatment with gemcitabine enhanced the production of IFN-γ and decreased the number of regulatory T cells. gemcitabine treatment increased IFN-γ production among CD4 T cells but not among CD8 T cells. The results indicated that GEM had immunoregulatory properties that might support immune response against cancer. This finding has implications for designing chemoimmunotherapy strategies. the Society for Free Radical Research Japan 2013-01 2012-11-20 /pmc/articles/PMC3541421/ /pubmed/23341700 http://dx.doi.org/10.3164/jcbn.12-60 Text en Copyright © 2013 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Sakai, Hiromi
Kokura, Satoshi
Ishikawa, Takeshi
Tsuchiya, Reiko
Okajima, Manabu
Matsuyama, Tatsuzou
Adachi, Satoko
Katada, Kazuhiro
Kamada, Kazuhiro
Uchiyama, Kazuhiko
Handa, Osamu
Takagi, Tomohisa
Yagi, Nobuaki
Naito, Yuji
Yoshikawa, Toshikazu
Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells
title Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells
title_full Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells
title_fullStr Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells
title_full_unstemmed Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells
title_short Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells
title_sort effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541421/
https://www.ncbi.nlm.nih.gov/pubmed/23341700
http://dx.doi.org/10.3164/jcbn.12-60
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