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PRBC-derived plasma induces non-muscle myosin type IIA-mediated neutrophil migration and morphologic change
CONTEXT: Neutrophils are the primary effector cells in the pathogenesis of transfusion-related acute lung injury or multiple organ failure after blood transfusion. OBJECTIVE: We aimed to investigate the effect of fresh (1 day preparation) and aged (42 day preparation) PRBC-derived plasma on neutroph...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Informa Healthcare
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541669/ https://www.ncbi.nlm.nih.gov/pubmed/23083320 http://dx.doi.org/10.3109/08923973.2012.677046 |
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author | Yu, Chen Xu, Li Chen, Lii-fang Guan, Ying-jie Kim, Minsoo Biffl, Walter L. Chin, Y. Eugene |
author_facet | Yu, Chen Xu, Li Chen, Lii-fang Guan, Ying-jie Kim, Minsoo Biffl, Walter L. Chin, Y. Eugene |
author_sort | Yu, Chen |
collection | PubMed |
description | CONTEXT: Neutrophils are the primary effector cells in the pathogenesis of transfusion-related acute lung injury or multiple organ failure after blood transfusion. OBJECTIVE: We aimed to investigate the effect of fresh (1 day preparation) and aged (42 day preparation) PRBC-derived plasma on neutrophil morphology, migration and phagocytosis. MATERIALS AND METHODS: We evaluated the production of reactive oxygen species (ROS) and the expression of non-muscle myosin heavy chain IIA (MYH9) in neutrophils treated with PRBC-derived plasma. We used western blots and antibody arrays to evaluate changes in signal transduction pathways in plasma-treated neutrophils. RESULTS: Aged PRBC-derived plasma elicited a stronger oxidative burst in neutrophils when compared with fresh PRBC-derived plasma (p < 0.05). Antibody arrays showed increased phosphorylation of NF-ĸB proteins (p105, p50 and Ikk) in aged PRBC-derived plasma-treated neutrophils. The expression of non-muscle myosin IIA (MYH9), a cytoskeleton protein involved in immune cell migration and morphological change, was also significantly upregulated in neutrophils treated with aged PRBC-derived plasma compared to fresh plasma (p < 0.05). Pretreatment of neutrophils with blebbistatin (a specific type II myosin inhibitor), ascorbic acid (an antioxidant), or staurosporine (a protein tyrosine kinase inhibitor), effectively abrogated the morphological changes, neutrophil migration, and phagocytosis induced by aged PRBC-derived plasma. CONCLUSION: Upregulation of MYH9 in neutrophils treated with aged PRBC-derived plasma and abrogation of neutrophil migration in blebbistatin-treated neutrophils suggested a functional role of MYH9 in the directional migration of immune cells. Our data help elucidate the cellular and molecular mechanisms of transfusion-related injury. |
format | Online Article Text |
id | pubmed-3541669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Informa Healthcare |
record_format | MEDLINE/PubMed |
spelling | pubmed-35416692013-01-15 PRBC-derived plasma induces non-muscle myosin type IIA-mediated neutrophil migration and morphologic change Yu, Chen Xu, Li Chen, Lii-fang Guan, Ying-jie Kim, Minsoo Biffl, Walter L. Chin, Y. Eugene Immunopharmacol Immunotoxicol Research Articles CONTEXT: Neutrophils are the primary effector cells in the pathogenesis of transfusion-related acute lung injury or multiple organ failure after blood transfusion. OBJECTIVE: We aimed to investigate the effect of fresh (1 day preparation) and aged (42 day preparation) PRBC-derived plasma on neutrophil morphology, migration and phagocytosis. MATERIALS AND METHODS: We evaluated the production of reactive oxygen species (ROS) and the expression of non-muscle myosin heavy chain IIA (MYH9) in neutrophils treated with PRBC-derived plasma. We used western blots and antibody arrays to evaluate changes in signal transduction pathways in plasma-treated neutrophils. RESULTS: Aged PRBC-derived plasma elicited a stronger oxidative burst in neutrophils when compared with fresh PRBC-derived plasma (p < 0.05). Antibody arrays showed increased phosphorylation of NF-ĸB proteins (p105, p50 and Ikk) in aged PRBC-derived plasma-treated neutrophils. The expression of non-muscle myosin IIA (MYH9), a cytoskeleton protein involved in immune cell migration and morphological change, was also significantly upregulated in neutrophils treated with aged PRBC-derived plasma compared to fresh plasma (p < 0.05). Pretreatment of neutrophils with blebbistatin (a specific type II myosin inhibitor), ascorbic acid (an antioxidant), or staurosporine (a protein tyrosine kinase inhibitor), effectively abrogated the morphological changes, neutrophil migration, and phagocytosis induced by aged PRBC-derived plasma. CONCLUSION: Upregulation of MYH9 in neutrophils treated with aged PRBC-derived plasma and abrogation of neutrophil migration in blebbistatin-treated neutrophils suggested a functional role of MYH9 in the directional migration of immune cells. Our data help elucidate the cellular and molecular mechanisms of transfusion-related injury. Informa Healthcare 2013-02 2012-10-19 /pmc/articles/PMC3541669/ /pubmed/23083320 http://dx.doi.org/10.3109/08923973.2012.677046 Text en © 2013 Informa Healthcare USA, Inc. http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the source is credited. |
spellingShingle | Research Articles Yu, Chen Xu, Li Chen, Lii-fang Guan, Ying-jie Kim, Minsoo Biffl, Walter L. Chin, Y. Eugene PRBC-derived plasma induces non-muscle myosin type IIA-mediated neutrophil migration and morphologic change |
title | PRBC-derived plasma induces non-muscle myosin type IIA-mediated neutrophil migration and morphologic change |
title_full | PRBC-derived plasma induces non-muscle myosin type IIA-mediated neutrophil migration and morphologic change |
title_fullStr | PRBC-derived plasma induces non-muscle myosin type IIA-mediated neutrophil migration and morphologic change |
title_full_unstemmed | PRBC-derived plasma induces non-muscle myosin type IIA-mediated neutrophil migration and morphologic change |
title_short | PRBC-derived plasma induces non-muscle myosin type IIA-mediated neutrophil migration and morphologic change |
title_sort | prbc-derived plasma induces non-muscle myosin type iia-mediated neutrophil migration and morphologic change |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541669/ https://www.ncbi.nlm.nih.gov/pubmed/23083320 http://dx.doi.org/10.3109/08923973.2012.677046 |
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