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Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata)

BACKGROUND: Brassica oleracea encompass a family of vegetables and cabbage that are among the most widely cultivated crops. In 2009, the B. oleracea Genome Sequencing Project was launched using next generation sequencing technology. None of the available maps were detailed enough to anchor the seque...

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Autores principales: Wang, Wanxing, Huang, Shunmou, Liu, Yumei, Fang, Zhiyuan, Yang, Limei, Hua, Wei, Yuan, Suxia, Liu, Shengyi, Sun, Jifeng, Zhuang, Mu, Zhang, Yangyong, Zeng, Aisong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542169/
https://www.ncbi.nlm.nih.gov/pubmed/23033896
http://dx.doi.org/10.1186/1471-2164-13-523
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author Wang, Wanxing
Huang, Shunmou
Liu, Yumei
Fang, Zhiyuan
Yang, Limei
Hua, Wei
Yuan, Suxia
Liu, Shengyi
Sun, Jifeng
Zhuang, Mu
Zhang, Yangyong
Zeng, Aisong
author_facet Wang, Wanxing
Huang, Shunmou
Liu, Yumei
Fang, Zhiyuan
Yang, Limei
Hua, Wei
Yuan, Suxia
Liu, Shengyi
Sun, Jifeng
Zhuang, Mu
Zhang, Yangyong
Zeng, Aisong
author_sort Wang, Wanxing
collection PubMed
description BACKGROUND: Brassica oleracea encompass a family of vegetables and cabbage that are among the most widely cultivated crops. In 2009, the B. oleracea Genome Sequencing Project was launched using next generation sequencing technology. None of the available maps were detailed enough to anchor the sequence scaffolds for the Genome Sequencing Project. This report describes the development of a large number of SSR and SNP markers from the whole genome shotgun sequence data of B. oleracea, and the construction of a high-density genetic linkage map using a double haploid mapping population. RESULTS: The B. oleracea high-density genetic linkage map that was constructed includes 1,227 markers in nine linkage groups spanning a total of 1197.9 cM with an average of 0.98 cM between adjacent loci. There were 602 SSR markers and 625 SNP markers on the map. The chromosome with the highest number of markers (186) was C03, and the chromosome with smallest number of markers (99) was C09. CONCLUSIONS: This first high-density map allowed the assembled scaffolds to be anchored to pseudochromosomes. The map also provides useful information for positional cloning, molecular breeding, and integration of information of genes and traits in B. oleracea. All the markers on the map will be transferable and could be used for the construction of other genetic maps.
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spelling pubmed-35421692013-01-11 Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata) Wang, Wanxing Huang, Shunmou Liu, Yumei Fang, Zhiyuan Yang, Limei Hua, Wei Yuan, Suxia Liu, Shengyi Sun, Jifeng Zhuang, Mu Zhang, Yangyong Zeng, Aisong BMC Genomics Research Article BACKGROUND: Brassica oleracea encompass a family of vegetables and cabbage that are among the most widely cultivated crops. In 2009, the B. oleracea Genome Sequencing Project was launched using next generation sequencing technology. None of the available maps were detailed enough to anchor the sequence scaffolds for the Genome Sequencing Project. This report describes the development of a large number of SSR and SNP markers from the whole genome shotgun sequence data of B. oleracea, and the construction of a high-density genetic linkage map using a double haploid mapping population. RESULTS: The B. oleracea high-density genetic linkage map that was constructed includes 1,227 markers in nine linkage groups spanning a total of 1197.9 cM with an average of 0.98 cM between adjacent loci. There were 602 SSR markers and 625 SNP markers on the map. The chromosome with the highest number of markers (186) was C03, and the chromosome with smallest number of markers (99) was C09. CONCLUSIONS: This first high-density map allowed the assembled scaffolds to be anchored to pseudochromosomes. The map also provides useful information for positional cloning, molecular breeding, and integration of information of genes and traits in B. oleracea. All the markers on the map will be transferable and could be used for the construction of other genetic maps. BioMed Central 2012-10-03 /pmc/articles/PMC3542169/ /pubmed/23033896 http://dx.doi.org/10.1186/1471-2164-13-523 Text en Copyright ©2012 Wang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Wanxing
Huang, Shunmou
Liu, Yumei
Fang, Zhiyuan
Yang, Limei
Hua, Wei
Yuan, Suxia
Liu, Shengyi
Sun, Jifeng
Zhuang, Mu
Zhang, Yangyong
Zeng, Aisong
Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata)
title Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata)
title_full Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata)
title_fullStr Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata)
title_full_unstemmed Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata)
title_short Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata)
title_sort construction and analysis of a high-density genetic linkage map in cabbage (brassica oleracea l. var. capitata)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542169/
https://www.ncbi.nlm.nih.gov/pubmed/23033896
http://dx.doi.org/10.1186/1471-2164-13-523
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