Cargando…
A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America
Cystic echinococcosis is still a major concern in South America. While some regions show advances in the control of the disease, others have among the highest incidence in the world. To reverse this situation the Pan American Health Organization (PAHO) has launched a regional project on cystic echin...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542170/ https://www.ncbi.nlm.nih.gov/pubmed/23326610 http://dx.doi.org/10.1371/journal.pntd.0001967 |
_version_ | 1782255466158489600 |
---|---|
author | Morel, Noelia Lassabe, Gabriel Elola, Susana Bondad, Mauricio Herrera, Silvia Marí, Carlos Last, Jerold A. Jensen, Oscar Gonzalez-Sapienza, Gualberto |
author_facet | Morel, Noelia Lassabe, Gabriel Elola, Susana Bondad, Mauricio Herrera, Silvia Marí, Carlos Last, Jerold A. Jensen, Oscar Gonzalez-Sapienza, Gualberto |
author_sort | Morel, Noelia |
collection | PubMed |
description | Cystic echinococcosis is still a major concern in South America. While some regions show advances in the control of the disease, others have among the highest incidence in the world. To reverse this situation the Pan American Health Organization (PAHO) has launched a regional project on cystic echinococcosis control and surveillance. An early concern of the program was the lack of a standardized diagnostic tool to monitor infection in dogs, a key target of control programs. Under this premise, we have developed a new copro-ELISA test after extensive screening of a large panel of monoclonal antibodies (MAbs) and polyclonal sera, which performs with high standards of sensitivity (92.6%) and specificity (86.4%) as established by necropsy diagnosis of dogs. The key component of the test, MAbEg9 has a convenient IgG isotype and reacts with a periodate-resistant epitope found in high molecular weight components of the worm. Time-course analysis of experimentally infected dogs showed that even animals with a very low number of parasites could be detected as early as day 20 post infection. The test was formulated in a ready-to-use kit format with proven stability of each component for a minimum of 3 months at room temperature. This characteristic facilitates its standardized use and shipping to other laboratories, which was demonstrated by the identical results obtained by two different laboratories in Peru and our own laboratory when a large number of field samples were analyzed independently in a blind fashion. |
format | Online Article Text |
id | pubmed-3542170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35421702013-01-16 A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America Morel, Noelia Lassabe, Gabriel Elola, Susana Bondad, Mauricio Herrera, Silvia Marí, Carlos Last, Jerold A. Jensen, Oscar Gonzalez-Sapienza, Gualberto PLoS Negl Trop Dis Research Article Cystic echinococcosis is still a major concern in South America. While some regions show advances in the control of the disease, others have among the highest incidence in the world. To reverse this situation the Pan American Health Organization (PAHO) has launched a regional project on cystic echinococcosis control and surveillance. An early concern of the program was the lack of a standardized diagnostic tool to monitor infection in dogs, a key target of control programs. Under this premise, we have developed a new copro-ELISA test after extensive screening of a large panel of monoclonal antibodies (MAbs) and polyclonal sera, which performs with high standards of sensitivity (92.6%) and specificity (86.4%) as established by necropsy diagnosis of dogs. The key component of the test, MAbEg9 has a convenient IgG isotype and reacts with a periodate-resistant epitope found in high molecular weight components of the worm. Time-course analysis of experimentally infected dogs showed that even animals with a very low number of parasites could be detected as early as day 20 post infection. The test was formulated in a ready-to-use kit format with proven stability of each component for a minimum of 3 months at room temperature. This characteristic facilitates its standardized use and shipping to other laboratories, which was demonstrated by the identical results obtained by two different laboratories in Peru and our own laboratory when a large number of field samples were analyzed independently in a blind fashion. Public Library of Science 2013-01-10 /pmc/articles/PMC3542170/ /pubmed/23326610 http://dx.doi.org/10.1371/journal.pntd.0001967 Text en © 2013 Morel et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Morel, Noelia Lassabe, Gabriel Elola, Susana Bondad, Mauricio Herrera, Silvia Marí, Carlos Last, Jerold A. Jensen, Oscar Gonzalez-Sapienza, Gualberto A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America |
title | A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America |
title_full | A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America |
title_fullStr | A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America |
title_full_unstemmed | A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America |
title_short | A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America |
title_sort | monoclonal antibody-based copro-elisa kit for canine echinococcosis to support the paho effort for hydatid disease control in south america |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542170/ https://www.ncbi.nlm.nih.gov/pubmed/23326610 http://dx.doi.org/10.1371/journal.pntd.0001967 |
work_keys_str_mv | AT morelnoelia amonoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT lassabegabriel amonoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT elolasusana amonoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT bondadmauricio amonoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT herrerasilvia amonoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT maricarlos amonoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT lastjerolda amonoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT jensenoscar amonoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT gonzalezsapienzagualberto amonoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT morelnoelia monoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT lassabegabriel monoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT elolasusana monoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT bondadmauricio monoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT herrerasilvia monoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT maricarlos monoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT lastjerolda monoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT jensenoscar monoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica AT gonzalezsapienzagualberto monoclonalantibodybasedcoproelisakitforcanineechinococcosistosupportthepahoeffortforhydatiddiseasecontrolinsouthamerica |