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A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America

Cystic echinococcosis is still a major concern in South America. While some regions show advances in the control of the disease, others have among the highest incidence in the world. To reverse this situation the Pan American Health Organization (PAHO) has launched a regional project on cystic echin...

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Autores principales: Morel, Noelia, Lassabe, Gabriel, Elola, Susana, Bondad, Mauricio, Herrera, Silvia, Marí, Carlos, Last, Jerold A., Jensen, Oscar, Gonzalez-Sapienza, Gualberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542170/
https://www.ncbi.nlm.nih.gov/pubmed/23326610
http://dx.doi.org/10.1371/journal.pntd.0001967
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author Morel, Noelia
Lassabe, Gabriel
Elola, Susana
Bondad, Mauricio
Herrera, Silvia
Marí, Carlos
Last, Jerold A.
Jensen, Oscar
Gonzalez-Sapienza, Gualberto
author_facet Morel, Noelia
Lassabe, Gabriel
Elola, Susana
Bondad, Mauricio
Herrera, Silvia
Marí, Carlos
Last, Jerold A.
Jensen, Oscar
Gonzalez-Sapienza, Gualberto
author_sort Morel, Noelia
collection PubMed
description Cystic echinococcosis is still a major concern in South America. While some regions show advances in the control of the disease, others have among the highest incidence in the world. To reverse this situation the Pan American Health Organization (PAHO) has launched a regional project on cystic echinococcosis control and surveillance. An early concern of the program was the lack of a standardized diagnostic tool to monitor infection in dogs, a key target of control programs. Under this premise, we have developed a new copro-ELISA test after extensive screening of a large panel of monoclonal antibodies (MAbs) and polyclonal sera, which performs with high standards of sensitivity (92.6%) and specificity (86.4%) as established by necropsy diagnosis of dogs. The key component of the test, MAbEg9 has a convenient IgG isotype and reacts with a periodate-resistant epitope found in high molecular weight components of the worm. Time-course analysis of experimentally infected dogs showed that even animals with a very low number of parasites could be detected as early as day 20 post infection. The test was formulated in a ready-to-use kit format with proven stability of each component for a minimum of 3 months at room temperature. This characteristic facilitates its standardized use and shipping to other laboratories, which was demonstrated by the identical results obtained by two different laboratories in Peru and our own laboratory when a large number of field samples were analyzed independently in a blind fashion.
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spelling pubmed-35421702013-01-16 A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America Morel, Noelia Lassabe, Gabriel Elola, Susana Bondad, Mauricio Herrera, Silvia Marí, Carlos Last, Jerold A. Jensen, Oscar Gonzalez-Sapienza, Gualberto PLoS Negl Trop Dis Research Article Cystic echinococcosis is still a major concern in South America. While some regions show advances in the control of the disease, others have among the highest incidence in the world. To reverse this situation the Pan American Health Organization (PAHO) has launched a regional project on cystic echinococcosis control and surveillance. An early concern of the program was the lack of a standardized diagnostic tool to monitor infection in dogs, a key target of control programs. Under this premise, we have developed a new copro-ELISA test after extensive screening of a large panel of monoclonal antibodies (MAbs) and polyclonal sera, which performs with high standards of sensitivity (92.6%) and specificity (86.4%) as established by necropsy diagnosis of dogs. The key component of the test, MAbEg9 has a convenient IgG isotype and reacts with a periodate-resistant epitope found in high molecular weight components of the worm. Time-course analysis of experimentally infected dogs showed that even animals with a very low number of parasites could be detected as early as day 20 post infection. The test was formulated in a ready-to-use kit format with proven stability of each component for a minimum of 3 months at room temperature. This characteristic facilitates its standardized use and shipping to other laboratories, which was demonstrated by the identical results obtained by two different laboratories in Peru and our own laboratory when a large number of field samples were analyzed independently in a blind fashion. Public Library of Science 2013-01-10 /pmc/articles/PMC3542170/ /pubmed/23326610 http://dx.doi.org/10.1371/journal.pntd.0001967 Text en © 2013 Morel et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Morel, Noelia
Lassabe, Gabriel
Elola, Susana
Bondad, Mauricio
Herrera, Silvia
Marí, Carlos
Last, Jerold A.
Jensen, Oscar
Gonzalez-Sapienza, Gualberto
A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America
title A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America
title_full A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America
title_fullStr A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America
title_full_unstemmed A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America
title_short A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America
title_sort monoclonal antibody-based copro-elisa kit for canine echinococcosis to support the paho effort for hydatid disease control in south america
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542170/
https://www.ncbi.nlm.nih.gov/pubmed/23326610
http://dx.doi.org/10.1371/journal.pntd.0001967
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