The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels

The pentaspan membrane glycoprotein CD133 (also known as prominin-1) has been widely used as a marker for both cancer and normal stem cells. However, the function of CD133 has not been elucidated. Here we describe a cancer stem cell line established from clear cell carcinoma of the ovary (CCC) and s...

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Detalles Bibliográficos
Autores principales: Koyama-Nasu, Ryo, Takahashi, Rina, Yanagida, Satoshi, Nasu-Nishimura, Yukiko, Oyama, Masaaki, Kozuka-Hata, Hiroko, Haruta, Ryo, Manabe, Emi, Hoshino-Okubo, Akemi, Omi, Hiroko, Yanaihara, Nozomu, Okamoto, Aikou, Tanaka, Tadao, Akiyama, Tetsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542344/
https://www.ncbi.nlm.nih.gov/pubmed/23326490
http://dx.doi.org/10.1371/journal.pone.0053710
Descripción
Sumario:The pentaspan membrane glycoprotein CD133 (also known as prominin-1) has been widely used as a marker for both cancer and normal stem cells. However, the function of CD133 has not been elucidated. Here we describe a cancer stem cell line established from clear cell carcinoma of the ovary (CCC) and show that CD133 interacts with plakoglobin (also known as γ-catenin), a desmosomal linker protein. We further demonstrate that knockdown of CD133 by RNA interference (RNAi) results in the downregulation of desmoglein-2, a desmosomal cadherin, and abrogates cell-cell adhesion and tumorigenicity of CCC stem cells. We speculate that CD133 may be a promising target for cancer chemotherapy.

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