The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels

The pentaspan membrane glycoprotein CD133 (also known as prominin-1) has been widely used as a marker for both cancer and normal stem cells. However, the function of CD133 has not been elucidated. Here we describe a cancer stem cell line established from clear cell carcinoma of the ovary (CCC) and s...

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Autores principales: Koyama-Nasu, Ryo, Takahashi, Rina, Yanagida, Satoshi, Nasu-Nishimura, Yukiko, Oyama, Masaaki, Kozuka-Hata, Hiroko, Haruta, Ryo, Manabe, Emi, Hoshino-Okubo, Akemi, Omi, Hiroko, Yanaihara, Nozomu, Okamoto, Aikou, Tanaka, Tadao, Akiyama, Tetsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542344/
https://www.ncbi.nlm.nih.gov/pubmed/23326490
http://dx.doi.org/10.1371/journal.pone.0053710
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author Koyama-Nasu, Ryo
Takahashi, Rina
Yanagida, Satoshi
Nasu-Nishimura, Yukiko
Oyama, Masaaki
Kozuka-Hata, Hiroko
Haruta, Ryo
Manabe, Emi
Hoshino-Okubo, Akemi
Omi, Hiroko
Yanaihara, Nozomu
Okamoto, Aikou
Tanaka, Tadao
Akiyama, Tetsu
author_facet Koyama-Nasu, Ryo
Takahashi, Rina
Yanagida, Satoshi
Nasu-Nishimura, Yukiko
Oyama, Masaaki
Kozuka-Hata, Hiroko
Haruta, Ryo
Manabe, Emi
Hoshino-Okubo, Akemi
Omi, Hiroko
Yanaihara, Nozomu
Okamoto, Aikou
Tanaka, Tadao
Akiyama, Tetsu
author_sort Koyama-Nasu, Ryo
collection PubMed
description The pentaspan membrane glycoprotein CD133 (also known as prominin-1) has been widely used as a marker for both cancer and normal stem cells. However, the function of CD133 has not been elucidated. Here we describe a cancer stem cell line established from clear cell carcinoma of the ovary (CCC) and show that CD133 interacts with plakoglobin (also known as γ-catenin), a desmosomal linker protein. We further demonstrate that knockdown of CD133 by RNA interference (RNAi) results in the downregulation of desmoglein-2, a desmosomal cadherin, and abrogates cell-cell adhesion and tumorigenicity of CCC stem cells. We speculate that CD133 may be a promising target for cancer chemotherapy.
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spelling pubmed-35423442013-01-16 The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels Koyama-Nasu, Ryo Takahashi, Rina Yanagida, Satoshi Nasu-Nishimura, Yukiko Oyama, Masaaki Kozuka-Hata, Hiroko Haruta, Ryo Manabe, Emi Hoshino-Okubo, Akemi Omi, Hiroko Yanaihara, Nozomu Okamoto, Aikou Tanaka, Tadao Akiyama, Tetsu PLoS One Research Article The pentaspan membrane glycoprotein CD133 (also known as prominin-1) has been widely used as a marker for both cancer and normal stem cells. However, the function of CD133 has not been elucidated. Here we describe a cancer stem cell line established from clear cell carcinoma of the ovary (CCC) and show that CD133 interacts with plakoglobin (also known as γ-catenin), a desmosomal linker protein. We further demonstrate that knockdown of CD133 by RNA interference (RNAi) results in the downregulation of desmoglein-2, a desmosomal cadherin, and abrogates cell-cell adhesion and tumorigenicity of CCC stem cells. We speculate that CD133 may be a promising target for cancer chemotherapy. Public Library of Science 2013-01-10 /pmc/articles/PMC3542344/ /pubmed/23326490 http://dx.doi.org/10.1371/journal.pone.0053710 Text en © 2013 Koyama-Nasu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Koyama-Nasu, Ryo
Takahashi, Rina
Yanagida, Satoshi
Nasu-Nishimura, Yukiko
Oyama, Masaaki
Kozuka-Hata, Hiroko
Haruta, Ryo
Manabe, Emi
Hoshino-Okubo, Akemi
Omi, Hiroko
Yanaihara, Nozomu
Okamoto, Aikou
Tanaka, Tadao
Akiyama, Tetsu
The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels
title The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels
title_full The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels
title_fullStr The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels
title_full_unstemmed The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels
title_short The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels
title_sort cancer stem cell marker cd133 interacts with plakoglobin and controls desmoglein-2 protein levels
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542344/
https://www.ncbi.nlm.nih.gov/pubmed/23326490
http://dx.doi.org/10.1371/journal.pone.0053710
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