(13)C-(13)C Homonuclear Recoupling in Solid-State Nuclear Magnetic Resonance at a Moderately High Magic-Angle-Spinning Frequency

Two-dimensional (13)C-(13)C correlation experiments are widely employed in structure determination of protein assemblies using solid-state nuclear magnetic resonance. Here, we investigate the process of (13)C-(13)C magnetisation transfer at a moderate magic-angle-spinning frequency of 30 kHz using s...

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Autores principales: Mithu, Venus Singh, Bakthavatsalam, Subha, Madhu, Perunthiruthy K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542364/
https://www.ncbi.nlm.nih.gov/pubmed/23326308
http://dx.doi.org/10.1371/journal.pone.0050504
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author Mithu, Venus Singh
Bakthavatsalam, Subha
Madhu, Perunthiruthy K.
author_facet Mithu, Venus Singh
Bakthavatsalam, Subha
Madhu, Perunthiruthy K.
author_sort Mithu, Venus Singh
collection PubMed
description Two-dimensional (13)C-(13)C correlation experiments are widely employed in structure determination of protein assemblies using solid-state nuclear magnetic resonance. Here, we investigate the process of (13)C-(13)C magnetisation transfer at a moderate magic-angle-spinning frequency of 30 kHz using some of the prominent second-order dipolar recoupling schemes. The effect of isotropic chemical-shift difference and spatial distance between two carbons and amplitude of radio frequency on (1)H channel on the magnetisation transfer efficiency of these schemes is discussed in detail.
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spelling pubmed-35423642013-01-16 (13)C-(13)C Homonuclear Recoupling in Solid-State Nuclear Magnetic Resonance at a Moderately High Magic-Angle-Spinning Frequency Mithu, Venus Singh Bakthavatsalam, Subha Madhu, Perunthiruthy K. PLoS One Research Article Two-dimensional (13)C-(13)C correlation experiments are widely employed in structure determination of protein assemblies using solid-state nuclear magnetic resonance. Here, we investigate the process of (13)C-(13)C magnetisation transfer at a moderate magic-angle-spinning frequency of 30 kHz using some of the prominent second-order dipolar recoupling schemes. The effect of isotropic chemical-shift difference and spatial distance between two carbons and amplitude of radio frequency on (1)H channel on the magnetisation transfer efficiency of these schemes is discussed in detail. Public Library of Science 2013-01-10 /pmc/articles/PMC3542364/ /pubmed/23326308 http://dx.doi.org/10.1371/journal.pone.0050504 Text en © 2013 Mithu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mithu, Venus Singh
Bakthavatsalam, Subha
Madhu, Perunthiruthy K.
(13)C-(13)C Homonuclear Recoupling in Solid-State Nuclear Magnetic Resonance at a Moderately High Magic-Angle-Spinning Frequency
title (13)C-(13)C Homonuclear Recoupling in Solid-State Nuclear Magnetic Resonance at a Moderately High Magic-Angle-Spinning Frequency
title_full (13)C-(13)C Homonuclear Recoupling in Solid-State Nuclear Magnetic Resonance at a Moderately High Magic-Angle-Spinning Frequency
title_fullStr (13)C-(13)C Homonuclear Recoupling in Solid-State Nuclear Magnetic Resonance at a Moderately High Magic-Angle-Spinning Frequency
title_full_unstemmed (13)C-(13)C Homonuclear Recoupling in Solid-State Nuclear Magnetic Resonance at a Moderately High Magic-Angle-Spinning Frequency
title_short (13)C-(13)C Homonuclear Recoupling in Solid-State Nuclear Magnetic Resonance at a Moderately High Magic-Angle-Spinning Frequency
title_sort (13)c-(13)c homonuclear recoupling in solid-state nuclear magnetic resonance at a moderately high magic-angle-spinning frequency
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542364/
https://www.ncbi.nlm.nih.gov/pubmed/23326308
http://dx.doi.org/10.1371/journal.pone.0050504
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