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Genome-Wide Analysis Reveals the Unique Stem Cell Identity of Human Amniocytes
Human amniotic fluid contains cells that potentially have important stem cell characteristics, yet the programs controlling their developmental potency are unclear. Here, we provide evidence that amniocytes derived from multiple patients are marked by heterogeneity and variability in expression leve...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542377/ https://www.ncbi.nlm.nih.gov/pubmed/23326421 http://dx.doi.org/10.1371/journal.pone.0053372 |
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author | Maguire, Colin T. Demarest, Bradley L. Hill, Jonathon T. Palmer, James D. Brothman, Arthur R. Yost, H. Joseph Condic, Maureen L. |
author_facet | Maguire, Colin T. Demarest, Bradley L. Hill, Jonathon T. Palmer, James D. Brothman, Arthur R. Yost, H. Joseph Condic, Maureen L. |
author_sort | Maguire, Colin T. |
collection | PubMed |
description | Human amniotic fluid contains cells that potentially have important stem cell characteristics, yet the programs controlling their developmental potency are unclear. Here, we provide evidence that amniocytes derived from multiple patients are marked by heterogeneity and variability in expression levels of pluripotency markers. Clonal analysis from multiple patients indicates that amniocytes have large pools of self-renewing cells that have an inherent property to give rise to a distinct amniocyte phenotype with a heterogeneity of pluripotent markers. Significant to their therapeutic potential, genome-wide profiles are distinct at different gestational ages and times in culture, but do not differ between genders. Based on hierarchical clustering and differential expression analyses of the entire transcriptome, amniocytes express canonical regulators associated with pluripotency and stem cell repression. Their profiles are distinct from human embryonic stem cells (ESCs), induced-pluripotent stem cells (iPSCs), and newborn foreskin fibroblasts. Amniocytes have a complex molecular signature, coexpressing trophoblastic, ectodermal, mesodermal, and endodermal cell-type-specific regulators. In contrast to the current view of the ground state of stem cells, ESCs and iPSCs also express high levels of a wide range of cell-type-specific regulators. The coexpression of multilineage differentiation markers combined with the strong expression of a subset of ES cell repressors in amniocytes suggests that these cells have a distinct phenotype that is unlike any other known cell-type or lineage. |
format | Online Article Text |
id | pubmed-3542377 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35423772013-01-16 Genome-Wide Analysis Reveals the Unique Stem Cell Identity of Human Amniocytes Maguire, Colin T. Demarest, Bradley L. Hill, Jonathon T. Palmer, James D. Brothman, Arthur R. Yost, H. Joseph Condic, Maureen L. PLoS One Research Article Human amniotic fluid contains cells that potentially have important stem cell characteristics, yet the programs controlling their developmental potency are unclear. Here, we provide evidence that amniocytes derived from multiple patients are marked by heterogeneity and variability in expression levels of pluripotency markers. Clonal analysis from multiple patients indicates that amniocytes have large pools of self-renewing cells that have an inherent property to give rise to a distinct amniocyte phenotype with a heterogeneity of pluripotent markers. Significant to their therapeutic potential, genome-wide profiles are distinct at different gestational ages and times in culture, but do not differ between genders. Based on hierarchical clustering and differential expression analyses of the entire transcriptome, amniocytes express canonical regulators associated with pluripotency and stem cell repression. Their profiles are distinct from human embryonic stem cells (ESCs), induced-pluripotent stem cells (iPSCs), and newborn foreskin fibroblasts. Amniocytes have a complex molecular signature, coexpressing trophoblastic, ectodermal, mesodermal, and endodermal cell-type-specific regulators. In contrast to the current view of the ground state of stem cells, ESCs and iPSCs also express high levels of a wide range of cell-type-specific regulators. The coexpression of multilineage differentiation markers combined with the strong expression of a subset of ES cell repressors in amniocytes suggests that these cells have a distinct phenotype that is unlike any other known cell-type or lineage. Public Library of Science 2013-01-10 /pmc/articles/PMC3542377/ /pubmed/23326421 http://dx.doi.org/10.1371/journal.pone.0053372 Text en © 2013 Maguire et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Maguire, Colin T. Demarest, Bradley L. Hill, Jonathon T. Palmer, James D. Brothman, Arthur R. Yost, H. Joseph Condic, Maureen L. Genome-Wide Analysis Reveals the Unique Stem Cell Identity of Human Amniocytes |
title | Genome-Wide Analysis Reveals the Unique Stem Cell Identity of Human Amniocytes |
title_full | Genome-Wide Analysis Reveals the Unique Stem Cell Identity of Human Amniocytes |
title_fullStr | Genome-Wide Analysis Reveals the Unique Stem Cell Identity of Human Amniocytes |
title_full_unstemmed | Genome-Wide Analysis Reveals the Unique Stem Cell Identity of Human Amniocytes |
title_short | Genome-Wide Analysis Reveals the Unique Stem Cell Identity of Human Amniocytes |
title_sort | genome-wide analysis reveals the unique stem cell identity of human amniocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542377/ https://www.ncbi.nlm.nih.gov/pubmed/23326421 http://dx.doi.org/10.1371/journal.pone.0053372 |
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