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Crystallographic snapshot of cellulose synthesis and membrane translocation

Cellulose, the most abundant biological macromolecule, is an extracellular, linear polymer of glucose molecules. It represents an essential component of plant cell walls but is also found in algae and bacteria. In bacteria, cellulose production frequently correlates with the formation of biofilms, a...

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Detalles Bibliográficos
Autores principales: Morgan, Jacob L.W., Strumillo, Joanna, Zimmer, Jochen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542415/
https://www.ncbi.nlm.nih.gov/pubmed/23222542
http://dx.doi.org/10.1038/nature11744
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author Morgan, Jacob L.W.
Strumillo, Joanna
Zimmer, Jochen
author_facet Morgan, Jacob L.W.
Strumillo, Joanna
Zimmer, Jochen
author_sort Morgan, Jacob L.W.
collection PubMed
description Cellulose, the most abundant biological macromolecule, is an extracellular, linear polymer of glucose molecules. It represents an essential component of plant cell walls but is also found in algae and bacteria. In bacteria, cellulose production frequently correlates with the formation of biofilms, a sessile, multicellular growth form. Cellulose synthesis and transport across the inner bacterial membrane is mediated by a complex of the multi-spanning catalytic BcsA subunit and the membrane-anchored, periplasmic BcsB protein. Here we present the crystal structure of a complex of BcsA and BcsB from Rhodobacter sphaeroides containing a translocating polysaccharide. The structure of the BcsA-B translocation intermediate reveals the architecture of the cellulose synthase, demonstrates how BcsA forms a cellulose-conducting channel, and suggests a model for the coupling of cellulose synthesis and translocation in which the nascent polysaccharide is extended by one glucose molecule at a time.
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spelling pubmed-35424152013-07-10 Crystallographic snapshot of cellulose synthesis and membrane translocation Morgan, Jacob L.W. Strumillo, Joanna Zimmer, Jochen Nature Article Cellulose, the most abundant biological macromolecule, is an extracellular, linear polymer of glucose molecules. It represents an essential component of plant cell walls but is also found in algae and bacteria. In bacteria, cellulose production frequently correlates with the formation of biofilms, a sessile, multicellular growth form. Cellulose synthesis and transport across the inner bacterial membrane is mediated by a complex of the multi-spanning catalytic BcsA subunit and the membrane-anchored, periplasmic BcsB protein. Here we present the crystal structure of a complex of BcsA and BcsB from Rhodobacter sphaeroides containing a translocating polysaccharide. The structure of the BcsA-B translocation intermediate reveals the architecture of the cellulose synthase, demonstrates how BcsA forms a cellulose-conducting channel, and suggests a model for the coupling of cellulose synthesis and translocation in which the nascent polysaccharide is extended by one glucose molecule at a time. 2012-12-09 2013-01-10 /pmc/articles/PMC3542415/ /pubmed/23222542 http://dx.doi.org/10.1038/nature11744 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Morgan, Jacob L.W.
Strumillo, Joanna
Zimmer, Jochen
Crystallographic snapshot of cellulose synthesis and membrane translocation
title Crystallographic snapshot of cellulose synthesis and membrane translocation
title_full Crystallographic snapshot of cellulose synthesis and membrane translocation
title_fullStr Crystallographic snapshot of cellulose synthesis and membrane translocation
title_full_unstemmed Crystallographic snapshot of cellulose synthesis and membrane translocation
title_short Crystallographic snapshot of cellulose synthesis and membrane translocation
title_sort crystallographic snapshot of cellulose synthesis and membrane translocation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542415/
https://www.ncbi.nlm.nih.gov/pubmed/23222542
http://dx.doi.org/10.1038/nature11744
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