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RACK1, a clue to the diagnosis of cutaneous melanomas in horses

BACKGROUND: Melanocytic proliferations are common in horses but the diagnosis of malignancy is not always straightforward. To improve diagnosis and prognosis, markers of malignancy are needed. Receptor for activated C kinase 1 (RACK1) protein may be such a marker. RACK1 was originally found to chara...

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Autores principales: Campagne, Cécile, Julé, Sophia, Bernex, Florence, Estrada, Mercedes, Aubin-Houzelstein, Geneviève, Panthier, Jean-Jacques, Egidy, Giorgia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543212/
https://www.ncbi.nlm.nih.gov/pubmed/22747534
http://dx.doi.org/10.1186/1746-6148-8-95
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author Campagne, Cécile
Julé, Sophia
Bernex, Florence
Estrada, Mercedes
Aubin-Houzelstein, Geneviève
Panthier, Jean-Jacques
Egidy, Giorgia
author_facet Campagne, Cécile
Julé, Sophia
Bernex, Florence
Estrada, Mercedes
Aubin-Houzelstein, Geneviève
Panthier, Jean-Jacques
Egidy, Giorgia
author_sort Campagne, Cécile
collection PubMed
description BACKGROUND: Melanocytic proliferations are common in horses but the diagnosis of malignancy is not always straightforward. To improve diagnosis and prognosis, markers of malignancy are needed. Receptor for activated C kinase 1 (RACK1) protein may be such a marker. RACK1 was originally found to characterize malignant melanocytic lesions in the Melanoblastoma-bearing Libechov minipig (MeLiM) and, later, in human patients. Our purpose was to investigate the value of RACK1 in the classification of cutaneous melanocytic proliferations in horses. RESULTS: Using immunofluorescence, we report here that both MITF (Microphthalmia-associated transcription factor) and PAX3 (Paired box 3) allow the identification of melanocytic cells in horse skin samples. Importantly, RACK1 was detected in melanocytic lesions but not in healthy skin melanocytes. Finally, we found that RACK1 labeling can be used in horses to distinguish benign melanocytic tumors from melanomas. Indeed, RACK1 labeling appeared more informative to assess malignancy than individual histomorphological features. CONCLUSIONS: This study confirms that horses provide an interesting model for melanoma genesis studies. It establishes MITF and PAX3 as markers of horse melanocytic cells. RACK1 emerges as an important marker of malignancy which may contribute to progress in the diagnosis of melanomas in both human and veterinary medicine.
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spelling pubmed-35432122013-01-14 RACK1, a clue to the diagnosis of cutaneous melanomas in horses Campagne, Cécile Julé, Sophia Bernex, Florence Estrada, Mercedes Aubin-Houzelstein, Geneviève Panthier, Jean-Jacques Egidy, Giorgia BMC Vet Res Research Article BACKGROUND: Melanocytic proliferations are common in horses but the diagnosis of malignancy is not always straightforward. To improve diagnosis and prognosis, markers of malignancy are needed. Receptor for activated C kinase 1 (RACK1) protein may be such a marker. RACK1 was originally found to characterize malignant melanocytic lesions in the Melanoblastoma-bearing Libechov minipig (MeLiM) and, later, in human patients. Our purpose was to investigate the value of RACK1 in the classification of cutaneous melanocytic proliferations in horses. RESULTS: Using immunofluorescence, we report here that both MITF (Microphthalmia-associated transcription factor) and PAX3 (Paired box 3) allow the identification of melanocytic cells in horse skin samples. Importantly, RACK1 was detected in melanocytic lesions but not in healthy skin melanocytes. Finally, we found that RACK1 labeling can be used in horses to distinguish benign melanocytic tumors from melanomas. Indeed, RACK1 labeling appeared more informative to assess malignancy than individual histomorphological features. CONCLUSIONS: This study confirms that horses provide an interesting model for melanoma genesis studies. It establishes MITF and PAX3 as markers of horse melanocytic cells. RACK1 emerges as an important marker of malignancy which may contribute to progress in the diagnosis of melanomas in both human and veterinary medicine. BioMed Central 2012-06-29 /pmc/articles/PMC3543212/ /pubmed/22747534 http://dx.doi.org/10.1186/1746-6148-8-95 Text en Copyright ©2012 Campagne et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Campagne, Cécile
Julé, Sophia
Bernex, Florence
Estrada, Mercedes
Aubin-Houzelstein, Geneviève
Panthier, Jean-Jacques
Egidy, Giorgia
RACK1, a clue to the diagnosis of cutaneous melanomas in horses
title RACK1, a clue to the diagnosis of cutaneous melanomas in horses
title_full RACK1, a clue to the diagnosis of cutaneous melanomas in horses
title_fullStr RACK1, a clue to the diagnosis of cutaneous melanomas in horses
title_full_unstemmed RACK1, a clue to the diagnosis of cutaneous melanomas in horses
title_short RACK1, a clue to the diagnosis of cutaneous melanomas in horses
title_sort rack1, a clue to the diagnosis of cutaneous melanomas in horses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543212/
https://www.ncbi.nlm.nih.gov/pubmed/22747534
http://dx.doi.org/10.1186/1746-6148-8-95
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