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Low-pressure pulsed focused ultrasound with microbubbles promotes an anticancer immunological response
BACKGROUND: High-intensity focused-ultrasound (HIFU) has been successfully employed for thermal ablation of tumors in clinical settings. Continuous- or pulsed-mode HIFU may also induce a host antitumor immune response, mainly through expansion of antigen-presenting cells in response to increased cel...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543346/ https://www.ncbi.nlm.nih.gov/pubmed/23140567 http://dx.doi.org/10.1186/1479-5876-10-221 |
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author | Liu, Hao-Li Hsieh, Han-Yi Lu, Li-An Kang, Chiao-Wen Wu, Ming-Fang Lin, Chun-Yen |
author_facet | Liu, Hao-Li Hsieh, Han-Yi Lu, Li-An Kang, Chiao-Wen Wu, Ming-Fang Lin, Chun-Yen |
author_sort | Liu, Hao-Li |
collection | PubMed |
description | BACKGROUND: High-intensity focused-ultrasound (HIFU) has been successfully employed for thermal ablation of tumors in clinical settings. Continuous- or pulsed-mode HIFU may also induce a host antitumor immune response, mainly through expansion of antigen-presenting cells in response to increased cellular debris and through increased macrophage activation/infiltration. Here we demonstrated that another form of focused ultrasound delivery, using low-pressure, pulsed-mode exposure in the presence of microbubbles (MBs), may also trigger an antitumor immunological response and inhibit tumor growth. METHODS: A total of 280 tumor-bearing animals were subjected to sonographically-guided FUS. Implanted tumors were exposed to low-pressure FUS (0.6 to 1.4 MPa) with MBs to increase the permeability of tumor microvasculature. RESULTS: Tumor progression was suppressed by both 0.6 and 1.4-MPa MB-enhanced FUS exposures. We observed a transient increase in infiltration of non-T regulatory (non-Treg) tumor infiltrating lymphocytes (TILs) and continual infiltration of CD8+ cytotoxic T-lymphocytes (CTL). The ratio of CD8+/Treg increased significantly and tumor growth was inhibited. CONCLUSIONS: Our findings suggest that low-pressure FUS exposure with MBs may constitute a useful tool for triggering an anticancer immune response, for potential cancer immunotherapy. |
format | Online Article Text |
id | pubmed-3543346 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35433462013-01-14 Low-pressure pulsed focused ultrasound with microbubbles promotes an anticancer immunological response Liu, Hao-Li Hsieh, Han-Yi Lu, Li-An Kang, Chiao-Wen Wu, Ming-Fang Lin, Chun-Yen J Transl Med Research BACKGROUND: High-intensity focused-ultrasound (HIFU) has been successfully employed for thermal ablation of tumors in clinical settings. Continuous- or pulsed-mode HIFU may also induce a host antitumor immune response, mainly through expansion of antigen-presenting cells in response to increased cellular debris and through increased macrophage activation/infiltration. Here we demonstrated that another form of focused ultrasound delivery, using low-pressure, pulsed-mode exposure in the presence of microbubbles (MBs), may also trigger an antitumor immunological response and inhibit tumor growth. METHODS: A total of 280 tumor-bearing animals were subjected to sonographically-guided FUS. Implanted tumors were exposed to low-pressure FUS (0.6 to 1.4 MPa) with MBs to increase the permeability of tumor microvasculature. RESULTS: Tumor progression was suppressed by both 0.6 and 1.4-MPa MB-enhanced FUS exposures. We observed a transient increase in infiltration of non-T regulatory (non-Treg) tumor infiltrating lymphocytes (TILs) and continual infiltration of CD8+ cytotoxic T-lymphocytes (CTL). The ratio of CD8+/Treg increased significantly and tumor growth was inhibited. CONCLUSIONS: Our findings suggest that low-pressure FUS exposure with MBs may constitute a useful tool for triggering an anticancer immune response, for potential cancer immunotherapy. BioMed Central 2012-11-11 /pmc/articles/PMC3543346/ /pubmed/23140567 http://dx.doi.org/10.1186/1479-5876-10-221 Text en Copyright ©2012 Liu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Liu, Hao-Li Hsieh, Han-Yi Lu, Li-An Kang, Chiao-Wen Wu, Ming-Fang Lin, Chun-Yen Low-pressure pulsed focused ultrasound with microbubbles promotes an anticancer immunological response |
title | Low-pressure pulsed focused ultrasound with microbubbles promotes an anticancer immunological response |
title_full | Low-pressure pulsed focused ultrasound with microbubbles promotes an anticancer immunological response |
title_fullStr | Low-pressure pulsed focused ultrasound with microbubbles promotes an anticancer immunological response |
title_full_unstemmed | Low-pressure pulsed focused ultrasound with microbubbles promotes an anticancer immunological response |
title_short | Low-pressure pulsed focused ultrasound with microbubbles promotes an anticancer immunological response |
title_sort | low-pressure pulsed focused ultrasound with microbubbles promotes an anticancer immunological response |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543346/ https://www.ncbi.nlm.nih.gov/pubmed/23140567 http://dx.doi.org/10.1186/1479-5876-10-221 |
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