Cargando…

Comparison Study of MS-HRM and Pyrosequencing Techniques for Quantification of APC and CDKN2A Gene Methylation

There is increasing interest in the development of cost-effective techniques for the quantification of DNA methylation biomarkers. We analyzed 90 samples of surgically resected colorectal cancer tissues for APC and CDKN2A promoter methylation using methylation sensitive-high resolution melting (MS-H...

Descripción completa

Detalles Bibliográficos
Autores principales:	Migheli, Francesca, Stoccoro, Andrea, Coppedè, Fabio, Wan Omar, Wan Adnan, Failli, Alessandra, Consolini, Rita, Seccia, Massimo, Spisni, Roberto, Miccoli, Paolo, Mathers, John C., Migliore, Lucia
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543439/ https://www.ncbi.nlm.nih.gov/pubmed/23326336 http://dx.doi.org/10.1371/journal.pone.0052501

Ejemplares similares

Efficacy and Feasibility of the Epithelial Cell Adhesion Molecule (EpCAM) Immunomagnetic Cell Sorter for Studies of DNA Methylation in Colorectal Cancer
por: Failli, Alessandra, et al.
Publicado: (2013)

Genetics, Cytogenetics, and Epigenetics of Colorectal Cancer
por: Migliore, Lucia, et al.
Publicado: (2011)

Defective Generation and Maturation of Dendritic Cells from Monocytes in Colorectal Cancer Patients during the Course of Disease
por: Orsini, Giulia, et al.
Publicado: (2013)

Dendritic cell defects in the colorectal cancer
por: Legitimo, Annalisa, et al.
Publicado: (2014)

Investigation of MLH1, MGMT, CDKN2A, and RASSF1A Gene Methylation in Thymomas From Patients With Myasthenia Gravis
por: Coppedè, Fabio, et al.
Publicado: (2020)

Plasma Homocysteine and Polymorphisms of Genes Involved in Folate Metabolism Correlate with DNMT1 Gene Methylation Levels
por: Coppedè, Fabio, et al.
Publicado: (2019)

MGMT promoter methylation determined by HRM in comparison to MSP and pyrosequencing for predicting high-grade glioma response
por: Switzeny, Olivier J., et al.
Publicado: (2016)

Susceptibility to Aneuploidy in Young Mothers of Down Syndrome Children
por: Migliore, Lucia, et al.
Publicado: (2009)

Mitoepigenetics and Neurodegenerative Diseases
por: Coppedè, Fabio, et al.
Publicado: (2019)

Mitochondrial DNA Methylation and Human Diseases
por: Stoccoro, Andrea, et al.
Publicado: (2021)

Polymorphisms in folate-metabolizing genes, chromosome damage, and risk of Down syndrome in Italian women: identification of key factors using artificial neural networks
por: Coppedè, Fabio, et al.
Publicado: (2010)

Association of Polymorphisms in Genes Involved in One-Carbon Metabolism with MTHFR Methylation Levels
por: Coppedè, Fabio, et al.
Publicado: (2019)

Methylation levels assessment with Methylation-Sensitive High-Resolution Melting (MS-HRM)
por: Samsø Mathiasen, Sally, et al.
Publicado: (2022)

Methylation Profile of Small Breast Cancer Tumors Evaluated by Modified MS–HRM
por: Krasnyi, Aleksey M., et al.
Publicado: (2023)

DNA Methylation Biomarkers for Young Children with Idiopathic Autism Spectrum Disorder: A Systematic Review
por: Stoccoro, Andrea, et al.
Publicado: (2023)

Editorial: Role of genetics, epigenetics, and environmental factors in human complex diseases from the mitochondrial point of view
por: Stoccoro, Andrea, et al.
Publicado: (2023)

Characterization of CDKN2A(p16) methylation and impact in colorectal cancer: systematic analysis using pyrosequencing
por: Bihl, Michel P, et al.
Publicado: (2012)

Pyrosequencing Analysis of APC Methylation Level in Human Prostate Tissues: A Molecular Marker for Prostate Cancer
por: Yoon, Hyung-Yoon, et al.
Publicado: (2013)

Strategic prospects for HRM
por: Tyson, Shaun
Publicado: (1995)

Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome
por: Draht, Muriel X. G., et al.
Publicado: (2016)

Editorial: Epigenetics of Neurodevelopmental, Neuromuscular and Neurodegenerative Disorders
por: Coppedè, Fabio, et al.
Publicado: (2022)

Increase in Mitochondrial D-Loop Region Methylation Levels in Mild Cognitive Impairment Individuals
por: Stoccoro, Andrea, et al.
Publicado: (2022)

High-resolution melting (HRM) re-analysis of a polyposis patients cohort reveals previously undetected heterozygous and mosaic APC gene mutations
por: Out, Astrid A., et al.
Publicado: (2015)

Detection and quantification of Spirocerca lupi by HRM qPCR in fecal samples from dogs with spirocercosis
por: Rojas, Alicia, et al.
Publicado: (2017)

Performance of Different Analytical Software Packages in Quantification of DNA Methylation by Pyrosequencing
por: Grasso, Chiara, et al.
Publicado: (2016)

AI revolution in HRM: the new scorecard
por: Upadhyay, Ashwani Kumar, et al.
Publicado: (2020)

Multilevel relational influences on HRM practices: a cross-country comparative reflective review of HRM practices in Asia
por: Malik, Ashish, et al.
Publicado: (2022)

Dynamic quantitative detection of ABC transporter family promoter methylation by MS-HRM for predicting MDR in pancreatic cancer
por: Yao, Lie, et al.
Publicado: (2018)

The effects of zoledronate on monocyte-derived dendritic cells from melanoma patients differ depending on the clinical stage of the disease
por: Failli, Alessandra, et al.
Publicado: (2014)

Mitochondrial D-Loop Region Methylation and Copy Number in Peripheral Blood DNA of Parkinson’s Disease Patients
por: Stoccoro, Andrea, et al.
Publicado: (2021)

Methylation-sensitive high resolution melting (MS-HRM): a new approach for sensitive and high-throughput assessment of methylation
por: Wojdacz, Tomasz K., et al.
Publicado: (2007)

DNA methylation analysis with methylation‐sensitive high‐resolution melting (MS‐HRM) reveals gene panel for glioma characteristics
por: Majchrzak‐Celińska, Aleksandra, et al.
Publicado: (2020)

Promoter Methylation of CDKN2A, RARβ, and RASSF1A in Non-Small Cell Lung Carcinoma: Quantitative Evaluation Using Pyrosequencing
por: Lee, Jung Uee, et al.
Publicado: (2012)

Performance of pyrosequencing versus MALDI-TOF MS in bacteria identification in chronic lung disease
por: Navrátilová, Lucie, et al.
Publicado: (2016)

HiRadMat HRM-45 experiment
por: Ordan, Julien Marius
Publicado: (2018)

What difference does ("good") HRM make?
por: Buchan, James
Publicado: (2004)

DNA Methylation in the Fields of Prenatal Diagnosis and Early Detection of Cancers
por: Coppedè, Fabio, et al.
Publicado: (2023)

Methylation enrichment pyrosequencing: combining the specificity of MSP with validation by pyrosequencing
por: Shaw, Richard J., et al.
Publicado: (2006)

Trace Level Quantification of 4-Methyl-1-nitrosopiperazin in Rifampicin Capsules by LC-MS/MS
por: Tao, Xiaosha, et al.
Publicado: (2022)

Investigation of the metabolic stability of olmutinib by validated LC-MS/MS: quantification in human plasma
por: Attwa, Mohamed W., et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_cibrjs8rcapahobarobc6drb86