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In vitro growth factor-induced bio engineering of mature articular cartilage

Articular cartilage maturation is the postnatal development process that adapts joint surfaces to their site-specific biomechanical demands. Maturation involves gross morphological changes that occur through a process of synchronised growth and resorption of cartilage and generally ends at sexual ma...

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Autores principales: Khan, Ilyas M., Francis, Lewis, Theobald, Peter S., Perni, Stefano, Young, Robert D., Prokopovich, Polina, Conlan, R. Steven, Archer, Charles W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543901/
https://www.ncbi.nlm.nih.gov/pubmed/23182922
http://dx.doi.org/10.1016/j.biomaterials.2012.09.076
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author Khan, Ilyas M.
Francis, Lewis
Theobald, Peter S.
Perni, Stefano
Young, Robert D.
Prokopovich, Polina
Conlan, R. Steven
Archer, Charles W.
author_facet Khan, Ilyas M.
Francis, Lewis
Theobald, Peter S.
Perni, Stefano
Young, Robert D.
Prokopovich, Polina
Conlan, R. Steven
Archer, Charles W.
author_sort Khan, Ilyas M.
collection PubMed
description Articular cartilage maturation is the postnatal development process that adapts joint surfaces to their site-specific biomechanical demands. Maturation involves gross morphological changes that occur through a process of synchronised growth and resorption of cartilage and generally ends at sexual maturity. The inability to induce maturation in biomaterial constructs designed for cartilage repair has been cited as a major cause for their failure in producing persistent cell-based repair of joint lesions. The combination of growth factors FGF2 and TGFβ1 induces accelerated articular cartilage maturation in vitro such that many molecular and morphological characteristics of tissue maturation are observable. We hypothesised that experimental growth factor-induced maturation of immature cartilage would result in a biophysical and biochemical composition consistent with a mature phenotype. Using native immature and mature cartilage as reference, we observed that growth factor-treated immature cartilages displayed increased nano-compressive stiffness, decreased surface adhesion, decreased water content, increased collagen content and smoother surfaces, correlating with a convergence to the mature cartilage phenotype. Furthermore, increased gene expression of surface structural protein collagen type I in growth factor-treated explants compared to reference cartilages demonstrates that they are still in the dynamic phase of the postnatal developmental transition. These data provide a basis for understanding the regulation of postnatal maturation of articular cartilage and the application of growth factor-induced maturation in vitro and in vivo in order to repair and regenerate cartilage defects.
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spelling pubmed-35439012013-02-01 In vitro growth factor-induced bio engineering of mature articular cartilage Khan, Ilyas M. Francis, Lewis Theobald, Peter S. Perni, Stefano Young, Robert D. Prokopovich, Polina Conlan, R. Steven Archer, Charles W. Biomaterials Article Articular cartilage maturation is the postnatal development process that adapts joint surfaces to their site-specific biomechanical demands. Maturation involves gross morphological changes that occur through a process of synchronised growth and resorption of cartilage and generally ends at sexual maturity. The inability to induce maturation in biomaterial constructs designed for cartilage repair has been cited as a major cause for their failure in producing persistent cell-based repair of joint lesions. The combination of growth factors FGF2 and TGFβ1 induces accelerated articular cartilage maturation in vitro such that many molecular and morphological characteristics of tissue maturation are observable. We hypothesised that experimental growth factor-induced maturation of immature cartilage would result in a biophysical and biochemical composition consistent with a mature phenotype. Using native immature and mature cartilage as reference, we observed that growth factor-treated immature cartilages displayed increased nano-compressive stiffness, decreased surface adhesion, decreased water content, increased collagen content and smoother surfaces, correlating with a convergence to the mature cartilage phenotype. Furthermore, increased gene expression of surface structural protein collagen type I in growth factor-treated explants compared to reference cartilages demonstrates that they are still in the dynamic phase of the postnatal developmental transition. These data provide a basis for understanding the regulation of postnatal maturation of articular cartilage and the application of growth factor-induced maturation in vitro and in vivo in order to repair and regenerate cartilage defects. Elsevier Science 2013-02 /pmc/articles/PMC3543901/ /pubmed/23182922 http://dx.doi.org/10.1016/j.biomaterials.2012.09.076 Text en © 2013 Elsevier Ltd. https://creativecommons.org/licenses/by/4.0/ Open Access under CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) license
spellingShingle Article
Khan, Ilyas M.
Francis, Lewis
Theobald, Peter S.
Perni, Stefano
Young, Robert D.
Prokopovich, Polina
Conlan, R. Steven
Archer, Charles W.
In vitro growth factor-induced bio engineering of mature articular cartilage
title In vitro growth factor-induced bio engineering of mature articular cartilage
title_full In vitro growth factor-induced bio engineering of mature articular cartilage
title_fullStr In vitro growth factor-induced bio engineering of mature articular cartilage
title_full_unstemmed In vitro growth factor-induced bio engineering of mature articular cartilage
title_short In vitro growth factor-induced bio engineering of mature articular cartilage
title_sort in vitro growth factor-induced bio engineering of mature articular cartilage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543901/
https://www.ncbi.nlm.nih.gov/pubmed/23182922
http://dx.doi.org/10.1016/j.biomaterials.2012.09.076
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