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In vitro assay for osteoinductive activity of different demineralized freeze-dried bone allograft

PURPOSE: Various bone graft materials have been used for periodontal tissue regeneration. Demineralized freeze-dried bone allograft (DFDBA) is a widely used bone substitute. The current widespread use of DFDBA is based on its potential osteoinductive ability. Due to the lack of verifiable data, the...

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Autores principales: Vaziri, Shahram, Vahabi, Surena, Torshabi, Maryam, Hematzadeh, Somayeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Academy of Periodontology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543938/
https://www.ncbi.nlm.nih.gov/pubmed/23346466
http://dx.doi.org/10.5051/jpis.2012.42.6.224
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author Vaziri, Shahram
Vahabi, Surena
Torshabi, Maryam
Hematzadeh, Somayeh
author_facet Vaziri, Shahram
Vahabi, Surena
Torshabi, Maryam
Hematzadeh, Somayeh
author_sort Vaziri, Shahram
collection PubMed
description PURPOSE: Various bone graft materials have been used for periodontal tissue regeneration. Demineralized freeze-dried bone allograft (DFDBA) is a widely used bone substitute. The current widespread use of DFDBA is based on its potential osteoinductive ability. Due to the lack of verifiable data, the purpose of this study was to assess the osteoinductive activity of different DFDBAs in vitro. METHODS: Sarcoma osteogenic (SaOS-2) cells (human osteoblast-like cells) were exposed to 8 mg/mL and 16 mg/mL concentrations of three commercial types of DFDBA: Osseo+, AlloOss, and Cenobone. The effect of these materials on cell proliferation was determined using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. The osteoinductive ability was evaluated using alizarin red staining, and the results were confirmed by evaluating osteogenic gene expression using reverse transcription polymerase chain reaction (RT-PCR). RESULTS: In the SaOS-2 cells, an 8 mg/mL concentration of Osseo+ and Cenobone significantly increased cell proliferation in 48 hours after exposure (P<0.001); however, in these two bone materials, the proliferation of cells was significantly decreased after 48 hours of exposure with a 16 mg/mL concentration (P<0.001). The alizarin red staining results demonstrated that the 16 mg/mL concentration of all three tested DFDBA induced complete morphologic differentiation and mineralized nodule production of the SaOS-2 cells. The RT-PCR results revealed osteopontin gene expression at a 16 mg/mL concentration of all three test groups, but not at an 8 mg/mL concentration. CONCLUSIONS: These commercial types of DFDBA are capable of decreasing proliferation and increasing osteogenic differentiation of the SaOS-2 cell line and have osteoinductive activity in vitro.
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spelling pubmed-35439382013-01-23 In vitro assay for osteoinductive activity of different demineralized freeze-dried bone allograft Vaziri, Shahram Vahabi, Surena Torshabi, Maryam Hematzadeh, Somayeh J Periodontal Implant Sci Research Article PURPOSE: Various bone graft materials have been used for periodontal tissue regeneration. Demineralized freeze-dried bone allograft (DFDBA) is a widely used bone substitute. The current widespread use of DFDBA is based on its potential osteoinductive ability. Due to the lack of verifiable data, the purpose of this study was to assess the osteoinductive activity of different DFDBAs in vitro. METHODS: Sarcoma osteogenic (SaOS-2) cells (human osteoblast-like cells) were exposed to 8 mg/mL and 16 mg/mL concentrations of three commercial types of DFDBA: Osseo+, AlloOss, and Cenobone. The effect of these materials on cell proliferation was determined using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. The osteoinductive ability was evaluated using alizarin red staining, and the results were confirmed by evaluating osteogenic gene expression using reverse transcription polymerase chain reaction (RT-PCR). RESULTS: In the SaOS-2 cells, an 8 mg/mL concentration of Osseo+ and Cenobone significantly increased cell proliferation in 48 hours after exposure (P<0.001); however, in these two bone materials, the proliferation of cells was significantly decreased after 48 hours of exposure with a 16 mg/mL concentration (P<0.001). The alizarin red staining results demonstrated that the 16 mg/mL concentration of all three tested DFDBA induced complete morphologic differentiation and mineralized nodule production of the SaOS-2 cells. The RT-PCR results revealed osteopontin gene expression at a 16 mg/mL concentration of all three test groups, but not at an 8 mg/mL concentration. CONCLUSIONS: These commercial types of DFDBA are capable of decreasing proliferation and increasing osteogenic differentiation of the SaOS-2 cell line and have osteoinductive activity in vitro. Korean Academy of Periodontology 2012-12 2012-12-31 /pmc/articles/PMC3543938/ /pubmed/23346466 http://dx.doi.org/10.5051/jpis.2012.42.6.224 Text en Copyright © 2012 Korean Academy of Periodontology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/).
spellingShingle Research Article
Vaziri, Shahram
Vahabi, Surena
Torshabi, Maryam
Hematzadeh, Somayeh
In vitro assay for osteoinductive activity of different demineralized freeze-dried bone allograft
title In vitro assay for osteoinductive activity of different demineralized freeze-dried bone allograft
title_full In vitro assay for osteoinductive activity of different demineralized freeze-dried bone allograft
title_fullStr In vitro assay for osteoinductive activity of different demineralized freeze-dried bone allograft
title_full_unstemmed In vitro assay for osteoinductive activity of different demineralized freeze-dried bone allograft
title_short In vitro assay for osteoinductive activity of different demineralized freeze-dried bone allograft
title_sort in vitro assay for osteoinductive activity of different demineralized freeze-dried bone allograft
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543938/
https://www.ncbi.nlm.nih.gov/pubmed/23346466
http://dx.doi.org/10.5051/jpis.2012.42.6.224
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