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Analgesic and Anti-Inflammatory Activities of the Ethanolic Extract of Artemisia morrisonensis Hayata in Mice

The aim of this study was to investigate the possible analgesic and anti-inflammatory mechanisms of the ethanolic extract of A. morrisonensis Hayata (AM(EtOH)). Two models were employed for evaluation of the analgesic effects: acetic acid-induced writhing response and formalin-induced paw licking. T...

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Autores principales: Chou, Shen-Chieh, Chiu, Yung-Jia, Chen, Chao-Jung, Lin, Ying-Chih, Wu, Chung-Hao, Chao, Chien-Ti, Chang, Ching-Wen, Peng, Wen-Huang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544374/
https://www.ncbi.nlm.nih.gov/pubmed/23346188
http://dx.doi.org/10.1155/2012/138954
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author Chou, Shen-Chieh
Chiu, Yung-Jia
Chen, Chao-Jung
Lin, Ying-Chih
Wu, Chung-Hao
Chao, Chien-Ti
Chang, Ching-Wen
Peng, Wen-Huang
author_facet Chou, Shen-Chieh
Chiu, Yung-Jia
Chen, Chao-Jung
Lin, Ying-Chih
Wu, Chung-Hao
Chao, Chien-Ti
Chang, Ching-Wen
Peng, Wen-Huang
author_sort Chou, Shen-Chieh
collection PubMed
description The aim of this study was to investigate the possible analgesic and anti-inflammatory mechanisms of the ethanolic extract of A. morrisonensis Hayata (AM(EtOH)). Two models were employed for evaluation of the analgesic effects: acetic acid-induced writhing response and formalin-induced paw licking. The results demonstrated that AM(EtOH) decreased writhing response for both the acetic acid assay and the licking time in the formalin test. The anti-inflammatory effect was evaluated by paw edema of mice induced by λ-carrageenan. AM(EtOH) significantly decreased induced paw edema three to four hours after λ-carrageenan injection. Additionally, the results indicated that the anti-inflammatory mechanism of AM(EtOH) may be due to the declined levels of nitric oxide (NO) and malondialdehyde (MDA) in the edematous paw. Furthermore, AM(EtOH) decreased the tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) levels, leading to the reduction of prostaglandins and subsequently alleviated edema. Isolation and purification of the AM(EtOH) extract determined p-hydroxyacetophenone to be a major component at 130 mg/g of extract. No mortality was observed in the acute toxicity test given at the dose of 10 g/kg. This study demonstrated the possible mechanisms for the analgesic and anti-inflammatory effects of AM(EtOH) for mice and provided evidence for the ethnobotanical uses of A. morrisonensis in treating inflammatory diseases.
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spelling pubmed-35443742013-01-23 Analgesic and Anti-Inflammatory Activities of the Ethanolic Extract of Artemisia morrisonensis Hayata in Mice Chou, Shen-Chieh Chiu, Yung-Jia Chen, Chao-Jung Lin, Ying-Chih Wu, Chung-Hao Chao, Chien-Ti Chang, Ching-Wen Peng, Wen-Huang Evid Based Complement Alternat Med Research Article The aim of this study was to investigate the possible analgesic and anti-inflammatory mechanisms of the ethanolic extract of A. morrisonensis Hayata (AM(EtOH)). Two models were employed for evaluation of the analgesic effects: acetic acid-induced writhing response and formalin-induced paw licking. The results demonstrated that AM(EtOH) decreased writhing response for both the acetic acid assay and the licking time in the formalin test. The anti-inflammatory effect was evaluated by paw edema of mice induced by λ-carrageenan. AM(EtOH) significantly decreased induced paw edema three to four hours after λ-carrageenan injection. Additionally, the results indicated that the anti-inflammatory mechanism of AM(EtOH) may be due to the declined levels of nitric oxide (NO) and malondialdehyde (MDA) in the edematous paw. Furthermore, AM(EtOH) decreased the tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) levels, leading to the reduction of prostaglandins and subsequently alleviated edema. Isolation and purification of the AM(EtOH) extract determined p-hydroxyacetophenone to be a major component at 130 mg/g of extract. No mortality was observed in the acute toxicity test given at the dose of 10 g/kg. This study demonstrated the possible mechanisms for the analgesic and anti-inflammatory effects of AM(EtOH) for mice and provided evidence for the ethnobotanical uses of A. morrisonensis in treating inflammatory diseases. Hindawi Publishing Corporation 2012 2012-12-29 /pmc/articles/PMC3544374/ /pubmed/23346188 http://dx.doi.org/10.1155/2012/138954 Text en Copyright © 2012 Shen-Chieh Chou et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chou, Shen-Chieh
Chiu, Yung-Jia
Chen, Chao-Jung
Lin, Ying-Chih
Wu, Chung-Hao
Chao, Chien-Ti
Chang, Ching-Wen
Peng, Wen-Huang
Analgesic and Anti-Inflammatory Activities of the Ethanolic Extract of Artemisia morrisonensis Hayata in Mice
title Analgesic and Anti-Inflammatory Activities of the Ethanolic Extract of Artemisia morrisonensis Hayata in Mice
title_full Analgesic and Anti-Inflammatory Activities of the Ethanolic Extract of Artemisia morrisonensis Hayata in Mice
title_fullStr Analgesic and Anti-Inflammatory Activities of the Ethanolic Extract of Artemisia morrisonensis Hayata in Mice
title_full_unstemmed Analgesic and Anti-Inflammatory Activities of the Ethanolic Extract of Artemisia morrisonensis Hayata in Mice
title_short Analgesic and Anti-Inflammatory Activities of the Ethanolic Extract of Artemisia morrisonensis Hayata in Mice
title_sort analgesic and anti-inflammatory activities of the ethanolic extract of artemisia morrisonensis hayata in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544374/
https://www.ncbi.nlm.nih.gov/pubmed/23346188
http://dx.doi.org/10.1155/2012/138954
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