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MicroRNA-142 is mutated in about 20% of diffuse large B-cell lymphoma
MicroRNAs (miRNAs) are short 18–23 nucleotide long noncoding RNAs that posttranscriptionally regulate gene expression by binding to mRNA. Our previous miRNA profiling of diffuse large B-cell lymphoma (DLBCL) revealed a mutation in the seed sequence of miR-142-3p. Further analysis now showed that miR...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544448/ https://www.ncbi.nlm.nih.gov/pubmed/23342264 http://dx.doi.org/10.1002/cam4.29 |
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author | Kwanhian, Wiyada Lenze, Dido Alles, Julia Motsch, Natalie Barth, Stephanie Döll, Celina Imig, Jochen Hummel, Michael Tinguely, Marianne Trivedi, Pankaj Lulitanond, Viraphong Meister, Gunter Renner, Christoph Grässer, Friedrich A |
author_facet | Kwanhian, Wiyada Lenze, Dido Alles, Julia Motsch, Natalie Barth, Stephanie Döll, Celina Imig, Jochen Hummel, Michael Tinguely, Marianne Trivedi, Pankaj Lulitanond, Viraphong Meister, Gunter Renner, Christoph Grässer, Friedrich A |
author_sort | Kwanhian, Wiyada |
collection | PubMed |
description | MicroRNAs (miRNAs) are short 18–23 nucleotide long noncoding RNAs that posttranscriptionally regulate gene expression by binding to mRNA. Our previous miRNA profiling of diffuse large B-cell lymphoma (DLBCL) revealed a mutation in the seed sequence of miR-142-3p. Further analysis now showed that miR-142 was mutated in 11 (19.64%) of the 56 DLBCL cases. Of these, one case had a mutation in both alleles, with the remainder being heterozygous. Four mutations were found in the mature miR-142-5p, four in the mature miR-142-3p, and three mutations affected the miR-142 precursor. Two mutations in the seed sequence redirected miR-142-3p to the mRNA of the transcriptional repressor ZEB2 and one of them also targeted the ZEB1 mRNA. However, the other mutations in the mature miR-142-3p did not influence either the ZEB1 or ZEB2 3′ untranslated region (3′ UTR). On the other hand, the mutations affecting the seed sequence of miR-142-3p resulted in a loss of responsiveness in the 3′ UTR of the known miR-142-3p targets RAC1 and ADCY9. In contrast to the mouse p300 gene, the human p300 gene was not found to be a target for miR-142-5p. In one case with a mutation of the precursor, we observed aberrant processing of the miR-142-5p. Our data suggest that the mutations in miR-142 probably lead to a loss rather than a gain of function. This is the first report describing mutations of a miRNA gene in a large percentage of a distinct lymphoma subtype. |
format | Online Article Text |
id | pubmed-3544448 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-35444482013-01-22 MicroRNA-142 is mutated in about 20% of diffuse large B-cell lymphoma Kwanhian, Wiyada Lenze, Dido Alles, Julia Motsch, Natalie Barth, Stephanie Döll, Celina Imig, Jochen Hummel, Michael Tinguely, Marianne Trivedi, Pankaj Lulitanond, Viraphong Meister, Gunter Renner, Christoph Grässer, Friedrich A Cancer Med Cancer Biology MicroRNAs (miRNAs) are short 18–23 nucleotide long noncoding RNAs that posttranscriptionally regulate gene expression by binding to mRNA. Our previous miRNA profiling of diffuse large B-cell lymphoma (DLBCL) revealed a mutation in the seed sequence of miR-142-3p. Further analysis now showed that miR-142 was mutated in 11 (19.64%) of the 56 DLBCL cases. Of these, one case had a mutation in both alleles, with the remainder being heterozygous. Four mutations were found in the mature miR-142-5p, four in the mature miR-142-3p, and three mutations affected the miR-142 precursor. Two mutations in the seed sequence redirected miR-142-3p to the mRNA of the transcriptional repressor ZEB2 and one of them also targeted the ZEB1 mRNA. However, the other mutations in the mature miR-142-3p did not influence either the ZEB1 or ZEB2 3′ untranslated region (3′ UTR). On the other hand, the mutations affecting the seed sequence of miR-142-3p resulted in a loss of responsiveness in the 3′ UTR of the known miR-142-3p targets RAC1 and ADCY9. In contrast to the mouse p300 gene, the human p300 gene was not found to be a target for miR-142-5p. In one case with a mutation of the precursor, we observed aberrant processing of the miR-142-5p. Our data suggest that the mutations in miR-142 probably lead to a loss rather than a gain of function. This is the first report describing mutations of a miRNA gene in a large percentage of a distinct lymphoma subtype. Blackwell Publishing Ltd 2012-10 2012-09-18 /pmc/articles/PMC3544448/ /pubmed/23342264 http://dx.doi.org/10.1002/cam4.29 Text en © 2012 The Authors. Published by Blackwell Publishing Ltd. http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Cancer Biology Kwanhian, Wiyada Lenze, Dido Alles, Julia Motsch, Natalie Barth, Stephanie Döll, Celina Imig, Jochen Hummel, Michael Tinguely, Marianne Trivedi, Pankaj Lulitanond, Viraphong Meister, Gunter Renner, Christoph Grässer, Friedrich A MicroRNA-142 is mutated in about 20% of diffuse large B-cell lymphoma |
title | MicroRNA-142 is mutated in about 20% of diffuse large B-cell lymphoma |
title_full | MicroRNA-142 is mutated in about 20% of diffuse large B-cell lymphoma |
title_fullStr | MicroRNA-142 is mutated in about 20% of diffuse large B-cell lymphoma |
title_full_unstemmed | MicroRNA-142 is mutated in about 20% of diffuse large B-cell lymphoma |
title_short | MicroRNA-142 is mutated in about 20% of diffuse large B-cell lymphoma |
title_sort | microrna-142 is mutated in about 20% of diffuse large b-cell lymphoma |
topic | Cancer Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544448/ https://www.ncbi.nlm.nih.gov/pubmed/23342264 http://dx.doi.org/10.1002/cam4.29 |
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