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Animal model of Mycoplasma fermentans respiratory infection

BACKGROUND: Mycoplasma fermentans has been associated with respiratory, genitourinary tract infections and rheumatoid diseases but its role as pathogen is controversial. The purpose of this study was to probe that Mycoplasma fermentans is able to produce respiratory tract infection and migrate to se...

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Autores principales: Yáñez, Antonio, Martínez-Ramos, Azucena, Calixto, Teresa, González-Matus, Francisco Javier, Rivera-Tapia, José Antonio, Giono, Silvia, Gil, Constantino, Cedillo, Lilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544566/
https://www.ncbi.nlm.nih.gov/pubmed/23298636
http://dx.doi.org/10.1186/1756-0500-6-9
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author Yáñez, Antonio
Martínez-Ramos, Azucena
Calixto, Teresa
González-Matus, Francisco Javier
Rivera-Tapia, José Antonio
Giono, Silvia
Gil, Constantino
Cedillo, Lilia
author_facet Yáñez, Antonio
Martínez-Ramos, Azucena
Calixto, Teresa
González-Matus, Francisco Javier
Rivera-Tapia, José Antonio
Giono, Silvia
Gil, Constantino
Cedillo, Lilia
author_sort Yáñez, Antonio
collection PubMed
description BACKGROUND: Mycoplasma fermentans has been associated with respiratory, genitourinary tract infections and rheumatoid diseases but its role as pathogen is controversial. The purpose of this study was to probe that Mycoplasma fermentans is able to produce respiratory tract infection and migrate to several organs on an experimental infection model in hamsters. One hundred and twenty six hamsters were divided in six groups (A-F) of 21 hamsters each. Animals of groups A, B, C were intratracheally injected with one of the mycoplasma strains: Mycoplasma fermentans P 140 (wild strain), Mycoplasma fermentans PG 18 (type strain) or Mycoplasma pneumoniae Eaton strain. Groups D, E, F were the negative, media, and sham controls. Fragments of trachea, lungs, kidney, heart, brain and spleen were cultured and used for the histopathological study. U frequency test was used to compare recovery of mycoplasmas from organs. RESULTS: Mycoplasmas were detected by culture and PCR. The three mycoplasma strains induced an interstitial pneumonia; they also migrated to several organs and persisted there for at least 50 days. Mycoplasma fermentans P 140 induced a more severe damage in lungs than Mycoplasma fermentans PG 18. Mycoplasma pneumoniae produced severe damage in lungs and renal damage. CONCLUSIONS: Mycoplasma fermentans induced a respiratory tract infection and persisted in different organs for several weeks in hamsters. This finding may help to explain the ability of Mycoplasma fermentans to induce pneumonia and chronic infectious diseases in humans.
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spelling pubmed-35445662013-01-16 Animal model of Mycoplasma fermentans respiratory infection Yáñez, Antonio Martínez-Ramos, Azucena Calixto, Teresa González-Matus, Francisco Javier Rivera-Tapia, José Antonio Giono, Silvia Gil, Constantino Cedillo, Lilia BMC Res Notes Research Article BACKGROUND: Mycoplasma fermentans has been associated with respiratory, genitourinary tract infections and rheumatoid diseases but its role as pathogen is controversial. The purpose of this study was to probe that Mycoplasma fermentans is able to produce respiratory tract infection and migrate to several organs on an experimental infection model in hamsters. One hundred and twenty six hamsters were divided in six groups (A-F) of 21 hamsters each. Animals of groups A, B, C were intratracheally injected with one of the mycoplasma strains: Mycoplasma fermentans P 140 (wild strain), Mycoplasma fermentans PG 18 (type strain) or Mycoplasma pneumoniae Eaton strain. Groups D, E, F were the negative, media, and sham controls. Fragments of trachea, lungs, kidney, heart, brain and spleen were cultured and used for the histopathological study. U frequency test was used to compare recovery of mycoplasmas from organs. RESULTS: Mycoplasmas were detected by culture and PCR. The three mycoplasma strains induced an interstitial pneumonia; they also migrated to several organs and persisted there for at least 50 days. Mycoplasma fermentans P 140 induced a more severe damage in lungs than Mycoplasma fermentans PG 18. Mycoplasma pneumoniae produced severe damage in lungs and renal damage. CONCLUSIONS: Mycoplasma fermentans induced a respiratory tract infection and persisted in different organs for several weeks in hamsters. This finding may help to explain the ability of Mycoplasma fermentans to induce pneumonia and chronic infectious diseases in humans. BioMed Central 2013-01-08 /pmc/articles/PMC3544566/ /pubmed/23298636 http://dx.doi.org/10.1186/1756-0500-6-9 Text en Copyright ©2013 Yañez et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yáñez, Antonio
Martínez-Ramos, Azucena
Calixto, Teresa
González-Matus, Francisco Javier
Rivera-Tapia, José Antonio
Giono, Silvia
Gil, Constantino
Cedillo, Lilia
Animal model of Mycoplasma fermentans respiratory infection
title Animal model of Mycoplasma fermentans respiratory infection
title_full Animal model of Mycoplasma fermentans respiratory infection
title_fullStr Animal model of Mycoplasma fermentans respiratory infection
title_full_unstemmed Animal model of Mycoplasma fermentans respiratory infection
title_short Animal model of Mycoplasma fermentans respiratory infection
title_sort animal model of mycoplasma fermentans respiratory infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544566/
https://www.ncbi.nlm.nih.gov/pubmed/23298636
http://dx.doi.org/10.1186/1756-0500-6-9
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