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Anti-Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru

BACKGROUND: Erythrocyte invasion by Plasmodium falciparum is a complex process that involves two families; Erythrocyte Binding-Like (EBL) and the Reticulocyte Binding-Like (PfRh) proteins. Antibodies that inhibit merozoite attachment and invasion are believed to be important in mediating naturally a...

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Autores principales: Villasis, Elizabeth, Lopez-Perez, Mary, Torres, Katherine, Gamboa, Dionicia, Neyra, Victor, Bendezu, Jorge, Tricoche, Nancy, Lobo, Cheryl, Vinetz, Joseph M, Lustigman, Sara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544580/
https://www.ncbi.nlm.nih.gov/pubmed/23110555
http://dx.doi.org/10.1186/1475-2875-11-361
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author Villasis, Elizabeth
Lopez-Perez, Mary
Torres, Katherine
Gamboa, Dionicia
Neyra, Victor
Bendezu, Jorge
Tricoche, Nancy
Lobo, Cheryl
Vinetz, Joseph M
Lustigman, Sara
author_facet Villasis, Elizabeth
Lopez-Perez, Mary
Torres, Katherine
Gamboa, Dionicia
Neyra, Victor
Bendezu, Jorge
Tricoche, Nancy
Lobo, Cheryl
Vinetz, Joseph M
Lustigman, Sara
author_sort Villasis, Elizabeth
collection PubMed
description BACKGROUND: Erythrocyte invasion by Plasmodium falciparum is a complex process that involves two families; Erythrocyte Binding-Like (EBL) and the Reticulocyte Binding-Like (PfRh) proteins. Antibodies that inhibit merozoite attachment and invasion are believed to be important in mediating naturally acquired immunity and immunity generated by parasite blood stage vaccine candidates. The hypotheses tested in this study were 1) that antibody responses against specific P. falciparum invasion ligands (EBL and PfRh) differ between symptomatic and asymptomatic individuals living in the low-transmission region of the Peruvian Amazon and 2), such antibody responses might have an association, either direct or indirect, with clinical immunity observed in asymptomatically parasitaemic individuals. METHODS: ELISA was used to assess antibody responses (IgG, IgG1 and IgG3) against recombinant P. falciparum invasion ligands of the EBL (EBA-175, EBA-181, EBA-140) and PfRh families (PfRh1, PfRh2a, PfRh2b, PfRh4 and PfRh5) in 45 individuals infected with P. falciparum from Peruvian Amazon. Individuals were classified as having symptomatic malaria (N=37) or asymptomatic infection (N=8). RESULTS: Antibody responses against both EBL and PfRh family proteins were significantly higher in asymptomatic compared to symptomatic individuals, demonstrating an association with clinical immunity. Significant differences in the total IgG responses were observed with EBA-175, EBA-181, PfRh2b, and MSP1(19) (as a control). IgG1 responses against EBA-181, PfRh2a and PfRh2b were significantly higher in the asymptomatic individuals. Total IgG antibody responses against PfRh1, PfRh2a, PfRh2b, PfRh5, EBA-175, EBA-181 and MSP1(19) proteins were negatively correlated with level of parasitaemia. IgG1 responses against EBA-181, PfRh2a and PfRh2b and IgG3 response for PfRh2a were also negatively correlated with parasitaemia. CONCLUSIONS: These data suggest that falciparum malaria patients who develop clinical immunity (asymptomatic parasitaemia) in a low transmission setting such as the Peruvian Amazon have antibody responses to defined P. falciparum invasion ligand proteins higher than those found in symptomatic (non-immune) patients. While these findings will have to be confirmed by larger studies, these results are consistent with a potential role for one or more of these invasion ligands as a component of an anti-P. falciparum vaccine in low-transmission malaria-endemic regions.
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spelling pubmed-35445802013-01-16 Anti-Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru Villasis, Elizabeth Lopez-Perez, Mary Torres, Katherine Gamboa, Dionicia Neyra, Victor Bendezu, Jorge Tricoche, Nancy Lobo, Cheryl Vinetz, Joseph M Lustigman, Sara Malar J Research BACKGROUND: Erythrocyte invasion by Plasmodium falciparum is a complex process that involves two families; Erythrocyte Binding-Like (EBL) and the Reticulocyte Binding-Like (PfRh) proteins. Antibodies that inhibit merozoite attachment and invasion are believed to be important in mediating naturally acquired immunity and immunity generated by parasite blood stage vaccine candidates. The hypotheses tested in this study were 1) that antibody responses against specific P. falciparum invasion ligands (EBL and PfRh) differ between symptomatic and asymptomatic individuals living in the low-transmission region of the Peruvian Amazon and 2), such antibody responses might have an association, either direct or indirect, with clinical immunity observed in asymptomatically parasitaemic individuals. METHODS: ELISA was used to assess antibody responses (IgG, IgG1 and IgG3) against recombinant P. falciparum invasion ligands of the EBL (EBA-175, EBA-181, EBA-140) and PfRh families (PfRh1, PfRh2a, PfRh2b, PfRh4 and PfRh5) in 45 individuals infected with P. falciparum from Peruvian Amazon. Individuals were classified as having symptomatic malaria (N=37) or asymptomatic infection (N=8). RESULTS: Antibody responses against both EBL and PfRh family proteins were significantly higher in asymptomatic compared to symptomatic individuals, demonstrating an association with clinical immunity. Significant differences in the total IgG responses were observed with EBA-175, EBA-181, PfRh2b, and MSP1(19) (as a control). IgG1 responses against EBA-181, PfRh2a and PfRh2b were significantly higher in the asymptomatic individuals. Total IgG antibody responses against PfRh1, PfRh2a, PfRh2b, PfRh5, EBA-175, EBA-181 and MSP1(19) proteins were negatively correlated with level of parasitaemia. IgG1 responses against EBA-181, PfRh2a and PfRh2b and IgG3 response for PfRh2a were also negatively correlated with parasitaemia. CONCLUSIONS: These data suggest that falciparum malaria patients who develop clinical immunity (asymptomatic parasitaemia) in a low transmission setting such as the Peruvian Amazon have antibody responses to defined P. falciparum invasion ligand proteins higher than those found in symptomatic (non-immune) patients. While these findings will have to be confirmed by larger studies, these results are consistent with a potential role for one or more of these invasion ligands as a component of an anti-P. falciparum vaccine in low-transmission malaria-endemic regions. BioMed Central 2012-10-30 /pmc/articles/PMC3544580/ /pubmed/23110555 http://dx.doi.org/10.1186/1475-2875-11-361 Text en Copyright ©2012 Villasis et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Villasis, Elizabeth
Lopez-Perez, Mary
Torres, Katherine
Gamboa, Dionicia
Neyra, Victor
Bendezu, Jorge
Tricoche, Nancy
Lobo, Cheryl
Vinetz, Joseph M
Lustigman, Sara
Anti-Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru
title Anti-Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru
title_full Anti-Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru
title_fullStr Anti-Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru
title_full_unstemmed Anti-Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru
title_short Anti-Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru
title_sort anti-plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, loreto, peru
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544580/
https://www.ncbi.nlm.nih.gov/pubmed/23110555
http://dx.doi.org/10.1186/1475-2875-11-361
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