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Pronuclear morphology evaluation in in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles: a retrospective clinical review

BACKGROUND: The assessment of the embryo quality is crucial to maintain an high pregnancy rate and to reduce the risk of multiple pregnancy. The evaluation of the pronuclear and nucleolar characteristics of human zygote have been proposed as an indicator of embryo development and chromosomal complem...

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Autores principales: Nicoli, Alessia, Capodanno, Francesco, Rondini, Ilaria, Valli, Barbara, Villani, Maria Teresa, Morini, Daria, De Pascalis, Leonardo, Palomba, Stefano, La Sala, Giovanni Battista
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544622/
https://www.ncbi.nlm.nih.gov/pubmed/23282023
http://dx.doi.org/10.1186/1757-2215-6-1
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author Nicoli, Alessia
Capodanno, Francesco
Rondini, Ilaria
Valli, Barbara
Villani, Maria Teresa
Morini, Daria
De Pascalis, Leonardo
Palomba, Stefano
La Sala, Giovanni Battista
author_facet Nicoli, Alessia
Capodanno, Francesco
Rondini, Ilaria
Valli, Barbara
Villani, Maria Teresa
Morini, Daria
De Pascalis, Leonardo
Palomba, Stefano
La Sala, Giovanni Battista
author_sort Nicoli, Alessia
collection PubMed
description BACKGROUND: The assessment of the embryo quality is crucial to maintain an high pregnancy rate and to reduce the risk of multiple pregnancy. The evaluation of the pronuclear and nucleolar characteristics of human zygote have been proposed as an indicator of embryo development and chromosomal complement. The aim of the current study was to assess the role of pronuclear morphology evaluation in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles. METHODS: Retrospective clinical analysis on 755 non-elective transfers of only one embryo (ET). Embryo assessment was performed in days 1 and 2. Clinical and biological data were recorded and analyzed according to embryo and/or pronuclear morphology. RESULTS: Both pronuclear and embryo morphology were significantly related to clinical pregnancy and live-birth rates. No significant difference in clinical pregnancy and live-birth rates was detected when the pronuclear and embryo morphology assessments were combined. Embryo morphology and maternal age were the only independent predictors of favorable outcome by logistic regression analysis. CONCLUSIONS: Pronuclear evaluation is effective to select the best zygotes if ET is performed at day 1, whereas it did not improve the clinical outcomes when combined with embryo morphology evaluation in day 2.
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spelling pubmed-35446222013-01-16 Pronuclear morphology evaluation in in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles: a retrospective clinical review Nicoli, Alessia Capodanno, Francesco Rondini, Ilaria Valli, Barbara Villani, Maria Teresa Morini, Daria De Pascalis, Leonardo Palomba, Stefano La Sala, Giovanni Battista J Ovarian Res Review BACKGROUND: The assessment of the embryo quality is crucial to maintain an high pregnancy rate and to reduce the risk of multiple pregnancy. The evaluation of the pronuclear and nucleolar characteristics of human zygote have been proposed as an indicator of embryo development and chromosomal complement. The aim of the current study was to assess the role of pronuclear morphology evaluation in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles. METHODS: Retrospective clinical analysis on 755 non-elective transfers of only one embryo (ET). Embryo assessment was performed in days 1 and 2. Clinical and biological data were recorded and analyzed according to embryo and/or pronuclear morphology. RESULTS: Both pronuclear and embryo morphology were significantly related to clinical pregnancy and live-birth rates. No significant difference in clinical pregnancy and live-birth rates was detected when the pronuclear and embryo morphology assessments were combined. Embryo morphology and maternal age were the only independent predictors of favorable outcome by logistic regression analysis. CONCLUSIONS: Pronuclear evaluation is effective to select the best zygotes if ET is performed at day 1, whereas it did not improve the clinical outcomes when combined with embryo morphology evaluation in day 2. BioMed Central 2013-01-03 /pmc/articles/PMC3544622/ /pubmed/23282023 http://dx.doi.org/10.1186/1757-2215-6-1 Text en Copyright ©2013 Nicoli et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Nicoli, Alessia
Capodanno, Francesco
Rondini, Ilaria
Valli, Barbara
Villani, Maria Teresa
Morini, Daria
De Pascalis, Leonardo
Palomba, Stefano
La Sala, Giovanni Battista
Pronuclear morphology evaluation in in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles: a retrospective clinical review
title Pronuclear morphology evaluation in in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles: a retrospective clinical review
title_full Pronuclear morphology evaluation in in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles: a retrospective clinical review
title_fullStr Pronuclear morphology evaluation in in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles: a retrospective clinical review
title_full_unstemmed Pronuclear morphology evaluation in in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles: a retrospective clinical review
title_short Pronuclear morphology evaluation in in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles: a retrospective clinical review
title_sort pronuclear morphology evaluation in in vitro fertilization (ivf) / intracytoplasmic sperm injection (icsi) cycles: a retrospective clinical review
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544622/
https://www.ncbi.nlm.nih.gov/pubmed/23282023
http://dx.doi.org/10.1186/1757-2215-6-1
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