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Over-Expression of VvWRKY1 in Grapevines Induces Expression of Jasmonic Acid Pathway-Related Genes and Confers Higher Tolerance to the Downy Mildew

Most WRKY transcription factors activate expression of defence genes in a salicylic acid- and/or jasmonic acid-dependent signalling pathway. We previously identified a WRKY gene, VvWRKY1, which is able to enhance tolerance to fungal pathogens when it is overexpressed in tobacco. The present work ana...

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Autores principales: Marchive, Chloé, Léon, Céline, Kappel, Christian, Coutos-Thévenot, Pierre, Corio-Costet, Marie-France, Delrot, Serge, Lauvergeat, Virginie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544825/
https://www.ncbi.nlm.nih.gov/pubmed/23342101
http://dx.doi.org/10.1371/journal.pone.0054185
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author Marchive, Chloé
Léon, Céline
Kappel, Christian
Coutos-Thévenot, Pierre
Corio-Costet, Marie-France
Delrot, Serge
Lauvergeat, Virginie
author_facet Marchive, Chloé
Léon, Céline
Kappel, Christian
Coutos-Thévenot, Pierre
Corio-Costet, Marie-France
Delrot, Serge
Lauvergeat, Virginie
author_sort Marchive, Chloé
collection PubMed
description Most WRKY transcription factors activate expression of defence genes in a salicylic acid- and/or jasmonic acid-dependent signalling pathway. We previously identified a WRKY gene, VvWRKY1, which is able to enhance tolerance to fungal pathogens when it is overexpressed in tobacco. The present work analyzes the effects of VvWRKY1 overexpression in grapevine. Microarray analysis showed that genes encoding defence-related proteins were up-regulated in the leaves of transgenic 35S::VvWRKY1 grapevines. Quantitative RT-PCR analysis confirmed that three genes putatively involved in jasmonic acid signalling pathway were overexpressed in the transgenic grapes. The ability of VvWRKY1 to trans-activate the promoters of these genes was demonstrated by transient expression in grape protoplasts. The resistance to the causal agent of downy mildew, Plasmopara viticola, was enhanced in the transgenic plants. These results show that VvWRKY1 can increase resistance of grapevine against the downy mildew through transcriptional reprogramming leading to activation of the jasmonic acid signalling pathway.
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spelling pubmed-35448252013-01-22 Over-Expression of VvWRKY1 in Grapevines Induces Expression of Jasmonic Acid Pathway-Related Genes and Confers Higher Tolerance to the Downy Mildew Marchive, Chloé Léon, Céline Kappel, Christian Coutos-Thévenot, Pierre Corio-Costet, Marie-France Delrot, Serge Lauvergeat, Virginie PLoS One Research Article Most WRKY transcription factors activate expression of defence genes in a salicylic acid- and/or jasmonic acid-dependent signalling pathway. We previously identified a WRKY gene, VvWRKY1, which is able to enhance tolerance to fungal pathogens when it is overexpressed in tobacco. The present work analyzes the effects of VvWRKY1 overexpression in grapevine. Microarray analysis showed that genes encoding defence-related proteins were up-regulated in the leaves of transgenic 35S::VvWRKY1 grapevines. Quantitative RT-PCR analysis confirmed that three genes putatively involved in jasmonic acid signalling pathway were overexpressed in the transgenic grapes. The ability of VvWRKY1 to trans-activate the promoters of these genes was demonstrated by transient expression in grape protoplasts. The resistance to the causal agent of downy mildew, Plasmopara viticola, was enhanced in the transgenic plants. These results show that VvWRKY1 can increase resistance of grapevine against the downy mildew through transcriptional reprogramming leading to activation of the jasmonic acid signalling pathway. Public Library of Science 2013-01-14 /pmc/articles/PMC3544825/ /pubmed/23342101 http://dx.doi.org/10.1371/journal.pone.0054185 Text en © 2013 Marchive et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Marchive, Chloé
Léon, Céline
Kappel, Christian
Coutos-Thévenot, Pierre
Corio-Costet, Marie-France
Delrot, Serge
Lauvergeat, Virginie
Over-Expression of VvWRKY1 in Grapevines Induces Expression of Jasmonic Acid Pathway-Related Genes and Confers Higher Tolerance to the Downy Mildew
title Over-Expression of VvWRKY1 in Grapevines Induces Expression of Jasmonic Acid Pathway-Related Genes and Confers Higher Tolerance to the Downy Mildew
title_full Over-Expression of VvWRKY1 in Grapevines Induces Expression of Jasmonic Acid Pathway-Related Genes and Confers Higher Tolerance to the Downy Mildew
title_fullStr Over-Expression of VvWRKY1 in Grapevines Induces Expression of Jasmonic Acid Pathway-Related Genes and Confers Higher Tolerance to the Downy Mildew
title_full_unstemmed Over-Expression of VvWRKY1 in Grapevines Induces Expression of Jasmonic Acid Pathway-Related Genes and Confers Higher Tolerance to the Downy Mildew
title_short Over-Expression of VvWRKY1 in Grapevines Induces Expression of Jasmonic Acid Pathway-Related Genes and Confers Higher Tolerance to the Downy Mildew
title_sort over-expression of vvwrky1 in grapevines induces expression of jasmonic acid pathway-related genes and confers higher tolerance to the downy mildew
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544825/
https://www.ncbi.nlm.nih.gov/pubmed/23342101
http://dx.doi.org/10.1371/journal.pone.0054185
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