Cargando…

Dialysis Purification of Integrase-DNA Complexes Provides High-Resolution Atomic Force Microscopy Images: Dimeric Recombinant HIV-1 Integrase Binding and Specific Looping on DNA

It remains difficult to obtain high-resolution atomic force microscopy images of HIV-1 integrase bound to DNA in a dimeric or tetrameric fashion. We therefore constructed specific target DNAs to assess HIV-1 integrase binding and purified the complex by dialysis prior to analysis. Our resulting atom...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tsuruyama, Tatsuaki, Nakai, Tonau, Ohmori, Rei, Ozeki, Munetaka, Tamaki, Keiji, Yoshikawa, Kenichi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544922/ https://www.ncbi.nlm.nih.gov/pubmed/23341952 http://dx.doi.org/10.1371/journal.pone.0053572

Ejemplares similares

In Vitro HIV-1 Selective Integration into the Target Sequence and Decoy-Effect of the Modified Sequence
por: Tsuruyama, Tatsuaki, et al.
Publicado: (2010)

In Vitro HIV-1 LTR Integration into T-Cell Activation Gene CD27 Segment and the Decoy Effect of Modified-Sequence DNA
por: Ohmori, Rei, et al.
Publicado: (2012)

HIV-1 Integrase-DNA Recognition Mechanisms
por: Kessl, Jacques J., et al.
Publicado: (2009)

Insight into the ERVK Integrase – Propensity for DNA Damage
por: Bray, Samantha, et al.
Publicado: (2016)

Integrase and integration: biochemical activities of HIV-1 integrase
por: Delelis, Olivier, et al.
Publicado: (2008)

Sorbin and SH3 Domain‐Containing Protein 2 Is Released From Infarcted Heart in the Very Early Phase: Proteomic Analysis of Cardiac Tissues From Patients
por: Kakimoto, Yu, et al.
Publicado: (2013)

In Vitro Murine Leukemia Retroviral Integration and Structure Fluctuation of Target DNA
por: Tsuruyama, Tatsuaki, et al.
Publicado: (2012)

Retrovirus Integrase-DNA Structure Elucidates Concerted Integration Mechanisms
por: Grandgenett, Duane, et al.
Publicado: (2010)

Engineered Hyperactive Integrase for Concerted HIV-1 DNA Integration
por: Li, Min, et al.
Publicado: (2014)

Directed DNA Shuffling of Retrovirus and Retrotransposon Integrase Protein Domains
por: Qi, Xiaojie, et al.
Publicado: (2013)

A Possible Role for the Asymmetric C-Terminal Domain Dimer of Rous Sarcoma Virus Integrase in Viral DNA Binding
por: Shi, Ke, et al.
Publicado: (2013)

Resistance to Integrase Inhibitors
por: Métifiot, Mathieu, et al.
Publicado: (2010)

Relationship between HIV integrase polymorphisms and integrase inhibitor susceptibility: An in silico analysis
por: Hutapea, Hotma Martogi Lorensi, et al.
Publicado: (2018)

Structural and sequencing analysis of local target DNA recognition by MLV integrase
por: Aiyer, Sriram, et al.
Publicado: (2015)

Long Dissociation of Bictegravir from HIV-1 Integrase-DNA Complexes
por: White, Kirsten L., et al.
Publicado: (2021)

Novel In Situ Pretreatment Method for Significantly Enhancing the Signal In MALDI-TOF MS of Formalin-Fixed Paraffin-Embedded Tissue Sections
por: Kakimoto, Yu, et al.
Publicado: (2012)

HIV-2 Integrase Variation in Integrase Inhibitor-Naïve Adults in Senegal, West Africa
por: Gottlieb, Geoffrey S., et al.
Publicado: (2011)

HIV-1 integrase tetramers are the antiviral target of pyridine-based allosteric integrase inhibitors
por: Koneru, Pratibha C, et al.
Publicado: (2019)

Integrase-RNA interactions underscore the critical role of integrase in HIV-1 virion morphogenesis
por: Elliott, Jennifer L, et al.
Publicado: (2020)

Prototype Foamy Virus Integrase Displays Unique Biochemical Activities among Retroviral Integrases
por: Rabe, Anthony J., et al.
Publicado: (2021)

New Applications for Phage Integrases
por: Fogg, Paul C.M., et al.
Publicado: (2014)

Localization of ASV Integrase-DNA Contacts by Site-Directed Crosslinking and their Structural Analysis
por: Peletskaya, Elena, et al.
Publicado: (2011)

DNA damage enhances integration of HIV-1 into macrophages by overcoming integrase inhibition
por: Koyama, Takayoshi, et al.
Publicado: (2013)

HIV-1 Integrase Strand Transfer Inhibitors with Reduced Susceptibility to Drug Resistant Mutant Integrases
por: Zhao, Xue Zhi, et al.
Publicado: (2016)

HIV-1 Integrase Inhibitors with Modifications That Affect Their Potencies against Drug Resistant Integrase Mutants
por: Smith, Steven J., et al.
Publicado: (2021)

A Symmetric Region of the HIV-1 Integrase Dimerization Interface Is Essential for Viral Replication
por: Serrao, Erik, et al.
Publicado: (2012)

A novel function for spumaretrovirus integrase: an early requirement for integrase-mediated cleavage of 2 LTR circles
por: Delelis, Olivier, et al.
Publicado: (2005)

Impact of the HIV integrase genetic context on the phenotypic expression and in vivo dynamics of integrase inhibitor resistance mutations
por: Nguyen, Nga, et al.
Publicado: (2013)

Molecular evolution of HIV-1 integrase during the 20 years prior to the first approval of integrase inhibitors
por: Meixenberger, Karolin, et al.
Publicado: (2017)

Pre-organized structure of viral DNA at the binding-processing site of HIV-1 integrase
por: Renisio, Jean-Guillaume, et al.
Publicado: (2005)

DNA binding and synapsis by the large C-terminal domain of ϕC31 integrase
por: McEwan, Andrew R., et al.
Publicado: (2009)

Unprocessed Viral DNA Could Be the Primary Target of the HIV-1 Integrase Inhibitor Raltegravir
por: Ammar, Farah F., et al.
Publicado: (2012)

DNA Physical Properties and Nucleosome Positions Are Major Determinants of HIV-1 Integrase Selectivity
por: Naughtin, Monica, et al.
Publicado: (2015)

A simplified mathematical model of directional DNA site-specific recombination by serine integrases
por: Pokhilko, Alexandra, et al.
Publicado: (2017)

Multimodal Functionalities of HIV-1 Integrase
por: Engelman, Alan N., et al.
Publicado: (2022)

Discovery of small-molecule HIV-1 fusion and integrase inhibitors oleuropein and hydroxytyrosol: Part I. Integrase inhibition
por: Lee-Huang, Sylvia, et al.
Publicado: (2007)

Characterization of natural polymorphic sites of the HIV-1 integrase before the introduction of HIV-1 integrase inhibitors in Germany
por: Meixenberger, Karolin, et al.
Publicado: (2014)

Mutations Located outside the Integrase Gene Can Confer Resistance to HIV-1 Integrase Strand Transfer Inhibitors
por: Malet, Isabelle, et al.
Publicado: (2017)

Inhibition of HIV-1 integrase nuclear import and replication by a peptide bearing integrase putative nuclear localization signal
por: Levin, Aviad, et al.
Publicado: (2009)

Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors
por: Sakkhachornphop, Supachai, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_22mq8h0dlb5moti0jfof88g8ot