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The ent-15α-Acetoxykaur-16-en-19-oic Acid Relaxes Rat Artery Mesenteric Superior via Endothelium-Dependent and Endothelium-Independent Mechanisms
The objective of the study was to investigate the mechanism of the relaxant activity of the ent-15α-acetoxykaur-16-en-19-oic acid (KA-acetoxy). In rat mesenteric artery rings, KA-acetoxy induced a concentration-dependent relaxation in vessels precontracted with phenylephrine. In the absence of endot...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545360/ https://www.ncbi.nlm.nih.gov/pubmed/23346202 http://dx.doi.org/10.1155/2012/472821 |
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author | Ribeiro, Êurica Adélia Nogueira Herculano, Edla de Azevedo da Costa, Cintia Danieli Ferreira Furtado, Fabiola Fialho da-Cunha, Emídio Vasconcelos Leitão Barbosa-Filho, José Maria da Silva, Marcelo Sobral de Medeiros, Isac Almeida |
author_facet | Ribeiro, Êurica Adélia Nogueira Herculano, Edla de Azevedo da Costa, Cintia Danieli Ferreira Furtado, Fabiola Fialho da-Cunha, Emídio Vasconcelos Leitão Barbosa-Filho, José Maria da Silva, Marcelo Sobral de Medeiros, Isac Almeida |
author_sort | Ribeiro, Êurica Adélia Nogueira |
collection | PubMed |
description | The objective of the study was to investigate the mechanism of the relaxant activity of the ent-15α-acetoxykaur-16-en-19-oic acid (KA-acetoxy). In rat mesenteric artery rings, KA-acetoxy induced a concentration-dependent relaxation in vessels precontracted with phenylephrine. In the absence of endothelium, the vasorelaxation was significantly shifted to the right without reduction of the maximum effect. Endothelium-dependent relaxation was significantly attenuated by pretreatment with L-NAME, an inhibitor of the NO-synthase (NOS), indomethacin, an inhibitor of the cyclooxygenase, L-NAME + indomethacin, atropine, a nonselective antagonist of the muscarinic receptors, ODQ, selective inhibitor of the guanylyl cyclase enzyme, or hydroxocobalamin, a nitric oxide scavenger. The relaxation was completely reversed in the presence of L-NAME + 1 mM L-arginine or L-arginine, an NO precursor. Diterpene-induced relaxation was not affected by TEA, a nonselective inhibitor of K+ channels. The KA-acetoxy antagonized CaCl(2)-induced contractions in a concentration-dependent manner and also inhibited an 80 mM KCl-induced contraction. The KA-acetoxy did not interfere with Ca(2+) release from intracellular stores. The vasorelaxant induced by KA-acetoxy seems to involve the inhibition of the Ca(2+) influx and also, at least in part, by endothelial muscarinic receptors activation, NO and PGI(2) release. |
format | Online Article Text |
id | pubmed-3545360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-35453602013-01-23 The ent-15α-Acetoxykaur-16-en-19-oic Acid Relaxes Rat Artery Mesenteric Superior via Endothelium-Dependent and Endothelium-Independent Mechanisms Ribeiro, Êurica Adélia Nogueira Herculano, Edla de Azevedo da Costa, Cintia Danieli Ferreira Furtado, Fabiola Fialho da-Cunha, Emídio Vasconcelos Leitão Barbosa-Filho, José Maria da Silva, Marcelo Sobral de Medeiros, Isac Almeida Evid Based Complement Alternat Med Research Article The objective of the study was to investigate the mechanism of the relaxant activity of the ent-15α-acetoxykaur-16-en-19-oic acid (KA-acetoxy). In rat mesenteric artery rings, KA-acetoxy induced a concentration-dependent relaxation in vessels precontracted with phenylephrine. In the absence of endothelium, the vasorelaxation was significantly shifted to the right without reduction of the maximum effect. Endothelium-dependent relaxation was significantly attenuated by pretreatment with L-NAME, an inhibitor of the NO-synthase (NOS), indomethacin, an inhibitor of the cyclooxygenase, L-NAME + indomethacin, atropine, a nonselective antagonist of the muscarinic receptors, ODQ, selective inhibitor of the guanylyl cyclase enzyme, or hydroxocobalamin, a nitric oxide scavenger. The relaxation was completely reversed in the presence of L-NAME + 1 mM L-arginine or L-arginine, an NO precursor. Diterpene-induced relaxation was not affected by TEA, a nonselective inhibitor of K+ channels. The KA-acetoxy antagonized CaCl(2)-induced contractions in a concentration-dependent manner and also inhibited an 80 mM KCl-induced contraction. The KA-acetoxy did not interfere with Ca(2+) release from intracellular stores. The vasorelaxant induced by KA-acetoxy seems to involve the inhibition of the Ca(2+) influx and also, at least in part, by endothelial muscarinic receptors activation, NO and PGI(2) release. Hindawi Publishing Corporation 2012 2012-12-27 /pmc/articles/PMC3545360/ /pubmed/23346202 http://dx.doi.org/10.1155/2012/472821 Text en Copyright © 2012 Êurica Adélia Nogueira Ribeiro et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ribeiro, Êurica Adélia Nogueira Herculano, Edla de Azevedo da Costa, Cintia Danieli Ferreira Furtado, Fabiola Fialho da-Cunha, Emídio Vasconcelos Leitão Barbosa-Filho, José Maria da Silva, Marcelo Sobral de Medeiros, Isac Almeida The ent-15α-Acetoxykaur-16-en-19-oic Acid Relaxes Rat Artery Mesenteric Superior via Endothelium-Dependent and Endothelium-Independent Mechanisms |
title | The ent-15α-Acetoxykaur-16-en-19-oic Acid Relaxes Rat Artery Mesenteric Superior via Endothelium-Dependent and Endothelium-Independent Mechanisms |
title_full | The ent-15α-Acetoxykaur-16-en-19-oic Acid Relaxes Rat Artery Mesenteric Superior via Endothelium-Dependent and Endothelium-Independent Mechanisms |
title_fullStr | The ent-15α-Acetoxykaur-16-en-19-oic Acid Relaxes Rat Artery Mesenteric Superior via Endothelium-Dependent and Endothelium-Independent Mechanisms |
title_full_unstemmed | The ent-15α-Acetoxykaur-16-en-19-oic Acid Relaxes Rat Artery Mesenteric Superior via Endothelium-Dependent and Endothelium-Independent Mechanisms |
title_short | The ent-15α-Acetoxykaur-16-en-19-oic Acid Relaxes Rat Artery Mesenteric Superior via Endothelium-Dependent and Endothelium-Independent Mechanisms |
title_sort | ent-15α-acetoxykaur-16-en-19-oic acid relaxes rat artery mesenteric superior via endothelium-dependent and endothelium-independent mechanisms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545360/ https://www.ncbi.nlm.nih.gov/pubmed/23346202 http://dx.doi.org/10.1155/2012/472821 |
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