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Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies
A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derive...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546048/ https://www.ncbi.nlm.nih.gov/pubmed/23335964 http://dx.doi.org/10.1371/journal.pone.0053446 |
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author | Bréard, Emmanuel Lara, Estelle Comtet, Loïc Viarouge, Cyril Doceul, Virginie Desprat, Alexandra Vitour, Damien Pozzi, Nathalie Cay, Ann Brigitte De Regge, Nick Pourquier, Philippe Schirrmeier, Horst Hoffmann, Bernd Beer, Martin Sailleau, Corinne Zientara, Stéphan |
author_facet | Bréard, Emmanuel Lara, Estelle Comtet, Loïc Viarouge, Cyril Doceul, Virginie Desprat, Alexandra Vitour, Damien Pozzi, Nathalie Cay, Ann Brigitte De Regge, Nick Pourquier, Philippe Schirrmeier, Horst Hoffmann, Bernd Beer, Martin Sailleau, Corinne Zientara, Stéphan |
author_sort | Bréard, Emmanuel |
collection | PubMed |
description | A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-flurorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine collected in France and Belgium before 2009. Overall agreement between VNT and ELISA was 98.9% and 98.3% between VNT and IFA, indicating a very good concordance between the different techniques. Although cross-reactions with other Orthobunyavirus from the Simbu serogroup viruses might occur, it is a highly sensitive, specific and robust ELISA-test validated to detect anti-SBV antibodies. This test can be applied for SBV sero-diagnostics and disease-surveillance studies in ruminant species in Europe. |
format | Online Article Text |
id | pubmed-3546048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35460482013-01-18 Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies Bréard, Emmanuel Lara, Estelle Comtet, Loïc Viarouge, Cyril Doceul, Virginie Desprat, Alexandra Vitour, Damien Pozzi, Nathalie Cay, Ann Brigitte De Regge, Nick Pourquier, Philippe Schirrmeier, Horst Hoffmann, Bernd Beer, Martin Sailleau, Corinne Zientara, Stéphan PLoS One Research Article A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-flurorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine collected in France and Belgium before 2009. Overall agreement between VNT and ELISA was 98.9% and 98.3% between VNT and IFA, indicating a very good concordance between the different techniques. Although cross-reactions with other Orthobunyavirus from the Simbu serogroup viruses might occur, it is a highly sensitive, specific and robust ELISA-test validated to detect anti-SBV antibodies. This test can be applied for SBV sero-diagnostics and disease-surveillance studies in ruminant species in Europe. Public Library of Science 2013-01-15 /pmc/articles/PMC3546048/ /pubmed/23335964 http://dx.doi.org/10.1371/journal.pone.0053446 Text en © 2013 Bréard et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bréard, Emmanuel Lara, Estelle Comtet, Loïc Viarouge, Cyril Doceul, Virginie Desprat, Alexandra Vitour, Damien Pozzi, Nathalie Cay, Ann Brigitte De Regge, Nick Pourquier, Philippe Schirrmeier, Horst Hoffmann, Bernd Beer, Martin Sailleau, Corinne Zientara, Stéphan Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies |
title | Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies |
title_full | Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies |
title_fullStr | Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies |
title_full_unstemmed | Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies |
title_short | Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies |
title_sort | validation of a commercially available indirect elisa using a nucleocapside recombinant protein for detection of schmallenberg virus antibodies |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546048/ https://www.ncbi.nlm.nih.gov/pubmed/23335964 http://dx.doi.org/10.1371/journal.pone.0053446 |
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