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Vacuum Foam Drying: An Alternative to Lyophilization for Biomolecule Preservation

Vacuum foam drying is evaluated as an alternative for lyophilization for enhanced process and storage stability of bovine serum albumin. The protein protective efficiency of different stabilizers was compared in vacuum foam drying and lyophilization. Sucrose mixtures produced better foam characters...

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Detalles Bibliográficos
Autores principales: Jangle, R. D., Pisal, S. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546342/
https://www.ncbi.nlm.nih.gov/pubmed/23325988
http://dx.doi.org/10.4103/0250-474X.103837
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author Jangle, R. D.
Pisal, S. S.
author_facet Jangle, R. D.
Pisal, S. S.
author_sort Jangle, R. D.
collection PubMed
description Vacuum foam drying is evaluated as an alternative for lyophilization for enhanced process and storage stability of bovine serum albumin. The protein protective efficiency of different stabilizers was compared in vacuum foam drying and lyophilization. Sucrose mixtures produced better foam characters than mannitol. Unlike calcium lactate, incorporation of polyvinyl pyrrolidone to sucrose synergistically enhanced the recovery of bovine serum albumin. The conformational stability and bovine serum albumin content further increased with sodium phosphate as compared to potassium phosphate. All sucrose mixtures, except calcium lactate showed large α-helix amide-I band at approximately 1656 cm(-1). The amorphous powder diffraction in case of sodium phosphate monobasic mixture retained maximum bovine serum albumin content. The crystallization of similar mixtures in lyophilization reduced its bovine serum albumin content. Vacuum foam drying showed better processing and storage stability of bovine serum albumin than lyophilization process. Hence vacuum foam drying is short, simple and industrially economical process for biomolecules preservation.
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spelling pubmed-35463422013-01-16 Vacuum Foam Drying: An Alternative to Lyophilization for Biomolecule Preservation Jangle, R. D. Pisal, S. S. Indian J Pharm Sci Research Paper Vacuum foam drying is evaluated as an alternative for lyophilization for enhanced process and storage stability of bovine serum albumin. The protein protective efficiency of different stabilizers was compared in vacuum foam drying and lyophilization. Sucrose mixtures produced better foam characters than mannitol. Unlike calcium lactate, incorporation of polyvinyl pyrrolidone to sucrose synergistically enhanced the recovery of bovine serum albumin. The conformational stability and bovine serum albumin content further increased with sodium phosphate as compared to potassium phosphate. All sucrose mixtures, except calcium lactate showed large α-helix amide-I band at approximately 1656 cm(-1). The amorphous powder diffraction in case of sodium phosphate monobasic mixture retained maximum bovine serum albumin content. The crystallization of similar mixtures in lyophilization reduced its bovine serum albumin content. Vacuum foam drying showed better processing and storage stability of bovine serum albumin than lyophilization process. Hence vacuum foam drying is short, simple and industrially economical process for biomolecules preservation. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3546342/ /pubmed/23325988 http://dx.doi.org/10.4103/0250-474X.103837 Text en Copyright: © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Jangle, R. D.
Pisal, S. S.
Vacuum Foam Drying: An Alternative to Lyophilization for Biomolecule Preservation
title Vacuum Foam Drying: An Alternative to Lyophilization for Biomolecule Preservation
title_full Vacuum Foam Drying: An Alternative to Lyophilization for Biomolecule Preservation
title_fullStr Vacuum Foam Drying: An Alternative to Lyophilization for Biomolecule Preservation
title_full_unstemmed Vacuum Foam Drying: An Alternative to Lyophilization for Biomolecule Preservation
title_short Vacuum Foam Drying: An Alternative to Lyophilization for Biomolecule Preservation
title_sort vacuum foam drying: an alternative to lyophilization for biomolecule preservation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546342/
https://www.ncbi.nlm.nih.gov/pubmed/23325988
http://dx.doi.org/10.4103/0250-474X.103837
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